中华神经外科杂志
中華神經外科雜誌
중화신경외과잡지
Chinese Journal of Neurosurgery
2012年
2期
162-165
,共4页
侯宗刚%刘佰运%罗伟%郝淑煜%张莹%武化云
侯宗剛%劉佰運%囉偉%郝淑煜%張瑩%武化雲
후종강%류백운%라위%학숙욱%장형%무화운
外伤性脑损伤%氧化应激%氢生理盐水%认知功能
外傷性腦損傷%氧化應激%氫生理鹽水%認知功能
외상성뇌손상%양화응격%경생리염수%인지공능
Traumatic brain injury%Oxidative stress%Hydrogen- rich saline%Cognitive function
目的 观察氢生理盐水对脑外伤后大鼠认知功能的改善作用,并初步探讨其可能机制.方法 应用液压打击损伤装置建立大鼠中度颅脑损伤模型,实验动物随机分为对照组、损伤+生理盐水组、损伤+含氢生理盐水组,采用Morris水迷宫法测定大鼠的认知功能表现；利用分光光度比色法、酶联免疫吸附法和Western blot等方法,分别检测受损同侧海马组织中丙二醛(MDA)、脑源性神经生长因子(BDNF)和突触蛋白Ⅰ的水平,并采用方差分析方法进行统计学分析.结果 Morris 水迷宫法显示腹腔注射含氢的生理盐水能够明显地提高大鼠的认知功能；在对受伤同侧大鼠的海马组织进行测定中,显示损伤后大鼠海马组织中MDA水平的升高及BDNF和突触蛋白Ⅰ水平的下降,而腹腔内注射含氢生理盐水能够明显改善受伤大鼠的认知功能,并能够降低MDA水平,提高BDNF和突触蛋白Ⅰ.结论 腹腔内注射含氢生理盐水能够通过抑制氧化损伤,改善颅脑损伤后动物的认知功能,而BDNF及其效应蛋白突触蛋白Ⅰ可能与其认知功能改善有关.
目的 觀察氫生理鹽水對腦外傷後大鼠認知功能的改善作用,併初步探討其可能機製.方法 應用液壓打擊損傷裝置建立大鼠中度顱腦損傷模型,實驗動物隨機分為對照組、損傷+生理鹽水組、損傷+含氫生理鹽水組,採用Morris水迷宮法測定大鼠的認知功能錶現；利用分光光度比色法、酶聯免疫吸附法和Western blot等方法,分彆檢測受損同側海馬組織中丙二醛(MDA)、腦源性神經生長因子(BDNF)和突觸蛋白Ⅰ的水平,併採用方差分析方法進行統計學分析.結果 Morris 水迷宮法顯示腹腔註射含氫的生理鹽水能夠明顯地提高大鼠的認知功能；在對受傷同側大鼠的海馬組織進行測定中,顯示損傷後大鼠海馬組織中MDA水平的升高及BDNF和突觸蛋白Ⅰ水平的下降,而腹腔內註射含氫生理鹽水能夠明顯改善受傷大鼠的認知功能,併能夠降低MDA水平,提高BDNF和突觸蛋白Ⅰ.結論 腹腔內註射含氫生理鹽水能夠通過抑製氧化損傷,改善顱腦損傷後動物的認知功能,而BDNF及其效應蛋白突觸蛋白Ⅰ可能與其認知功能改善有關.
목적 관찰경생리염수대뇌외상후대서인지공능적개선작용,병초보탐토기가능궤제.방법 응용액압타격손상장치건립대서중도로뇌손상모형,실험동물수궤분위대조조、손상+생리염수조、손상+함경생리염수조,채용Morris수미궁법측정대서적인지공능표현；이용분광광도비색법、매련면역흡부법화Western blot등방법,분별검측수손동측해마조직중병이철(MDA)、뇌원성신경생장인자(BDNF)화돌촉단백Ⅰ적수평,병채용방차분석방법진행통계학분석.결과 Morris 수미궁법현시복강주사함경적생리염수능구명현지제고대서적인지공능；재대수상동측대서적해마조직진행측정중,현시손상후대서해마조직중MDA수평적승고급BDNF화돌촉단백Ⅰ수평적하강,이복강내주사함경생리염수능구명현개선수상대서적인지공능,병능구강저MDA수평,제고BDNF화돌촉단백Ⅰ.결론 복강내주사함경생리염수능구통과억제양화손상,개선로뇌손상후동물적인지공능,이BDNF급기효응단백돌촉단백Ⅰ가능여기인지공능개선유관.
Objective To investigate the effects of hydrogen- rich saline on cognitive function and its mechanisms.Methods Models of fluid percussion injury (FPI) were established.Rats were randomized into normal control group,FPI + saline group and FPI + hydrogen - rich saline group.The cognitive function was evaluated by Morris test; also spectrophotometric assay,ELISA and western blot were used respectively to measure MDA (malondialdehyde),BDNF (brain derived neurophic factor ) and synapsin I in hippocampus.Results After FPI,rats treated with hydrogen - rich saline showed significant improvement in cognition compared with no hydrogen - rich treated rats ( P ＜ 0.05 ) ; also MDA in hippocampus increased and BDNF and synapsin I decreased in the FPI + saline group ( P ＜ 0.05 ) ; Compared with FPI + saline group,MDA of hydrogen - rich saline group was lower,while BDNF and synapsin I were higher ( P ＜0.05).Conclusions With injected intraperitoneally,hydrogen- rich saline showed the protective effects on cognitive function,with the BDNF and synapsin I be involved.
