CAJ | 학술논문

目的探讨褪黑素(Mel)对过氧化氢(H202)氧化损伤的人视网膜色素上皮(hRPE)细胞的保护作用及其作用机制.方法实验研究.采用600μmoL/L H2O2建立体外培养的hRPE细胞氧化损伤模型.实验分为6组:溶剂对照组、600μmoL/L H2O2+溶剂组(H2O2损伤模型组)、600μmoL/L H2O2+10-7mol/L Mel组、600μmoL/L H2O2+10-6moL/L Mel组、600 μmol/L H2O2+10-5mol/L Mel组、600μmol/L H2O2+10-4moL/L Mel组.通过四甲基偶氮唑盐(MTT))法检测细胞活性;测定细胞内超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量以反映细胞氧化损伤程度;分别用DNA Ladders电泳法和流式细胞仪检测细胞的凋亡情况.溶剂对照组与600μmol/L H2O2组间均数比较采用随机区组设计的t检验;600μmol/L H2O2组以及600μmol/L H2O2+不同浓度Mel组间均数比较采用单因素5水平设计的方差分析,组间两两比较采用LSD-t检验.结果 H2O2模型组较对照组细胞活性明显降低、SOD活件降低、MDA含量增加、凋亡率升高,差异均有统计学意义(t=2.25,39.50,68.42;P<0.05);Mel干预组较模型组细胞活性升高、SOD活性升高、MDA含最减少、凋亡率降低,差异有统计学意义(P<0.05),并与药物浓度的变化呈正相关趋势.结论 Mel对H2O2诱导的RPE的氧化损伤具有保护作用,其机制可能与影响细胞活性、增强抗氧化酶活性、减少细胞凋亡有关.
목적 탐토퇴흑소(Mel)대과양화경(H202)양화손상적인시망막색소상피(hRPE)세포적보호작용급기작용궤제.방법 실험연구.채용600μmoL/L H2O2건입체외배양적hRPE세포양화손상모형.실험분위6조:용제대조조、600μmoL/L H2O2+용제조(H2O2손상모형조)、600μmoL/L H2O2+10-7mol/L Mel조、600μmoL/L H2O2+10-6moL/L Mel조、600 μmol/L H2O2+10-5mol/L Mel조、600μmol/L H2O2+10-4moL/L Mel조.통과사갑기우담서염(MTT))법검측세포활성;측정세포내초양화물기화매(SOD)활성화병이철(MDA)함량이반영세포양화손상정도;분별용DNA Ladders전영법화류식세포의검측세포적조망정황.용제대조조여600μmol/L H2O2조간균수비교채용수궤구조설계적t검험;600μmol/L H2O2조이급600μmol/L H2O2+불동농도Mel조간균수비교채용단인소5수평설계적방차분석,조간량량비교채용LSD-t검험.결과 H2O2모형조교대조조세포활성명현강저、SOD활건강저、MDA함량증가、조망솔승고,차이균유통계학의의(t=2.25,39.50,68.42;P<0.05);Mel간예조교모형조세포활성승고、SOD활성승고、MDA함최감소、조망솔강저,차이유통계학의의(P<0.05),병여약물농도적변화정정상관추세.결론 Mel대H2O2유도적RPE적양화손상구유보호작용,기궤제가능여영향세포활성、증강항양화매활성、감소세포조망유관.
Objective To investigate the protective effects of melatonin on the retinal pigment epithelium (RPE) against oxidative damage induced by hydrogen dioxide (H2O2) and its mechanism. Methods The RPE cells were seeded and divided into normol control group, oxidative damage group and the treatment group (treated with melatonin at the concentration of 1×10.7 mol/L,1×10-6 mol/L, 1×10-5 mol/L and 1×10-4 mol/L). The model of oxidative damage on the RPE cells was established by culturing the RPE cells with H2O2 at the concentration of 600 μmol/L for 1 hour in vitro. The cell viability of RPE cells was detected by the methyl thiazolyl tetrazolium (MTT) method. The degree of oxidative damage was evaluated by detecting the superoxide dismutase (SOD) and maleic dialdehyde (MDA). Apoptosis was detected qualitatively using the DNA Ladders electrophoretic mothed, and quantitatively using the Annexin V-FITC/PI double staining flow cytometry. Results Compared with normal control group, the oxidative damage group had low cell viability, low SOD and high MDA contents, and high apoptosis rate(t=2.25,39.50,68.42;P<0.05). Compared with oxidative damage group, the treatment group had high cell viability, high SOD and low M DA contents, and low apoptosis rate (P<0.05). Conclusions Melatonin has a protective effect on the RPE against oxidative damage induced by H2O2. The mechanism may involve in reinforcing the cell viability, strengthening the activity of antioxidase, and reducing the apoptosis.