CAJ | 학술논문

exocyst复合物在分泌过程中起重要作用.可是分子机理尚未研究透彻.通过TMP/UV的基因敲除方法,得到了exocyst复合物中一种组分sec-10的缺陷犁线虫.药学结果显示这种线虫神经信号传递存在缺陷,可是电生理的方法证明在神经肌肉接头处的已报道离子型受体和野生型相比并未发生改变.因此猜测,sec-10并未直接影响神经肌肉接头处的离子型受体,而是通过其他途径来行使功能.
exocyst복합물재분비과정중기중요작용.가시분자궤리상미연구투철.통과TMP/UV적기인고제방법,득도료exocyst복합물중일충조분sec-10적결함리선충.약학결과현시저충선충신경신호전체존재결함,가시전생리적방법증명재신경기육접두처적이보도리자형수체화야생형상비병미발생개변.인차시측,sec-10병미직접영향신경기육접두처적리자형수체,이시통과기타도경래행사공능.
Exocyst complex is known to function in the exocytosis network, however, the molecular mechanism is unclear yet. Using UV/trimethylpsoralen mutagenesis, the sec-10 (one component of the exocyst complex) knockout mutant of C. elegans was obtained for the first time. The drug sensitive assays revealed clearly that the sec-10 gene affected the neural signal transmission, however, the electrophysiological assay showed the function of the ionotropic receptors in the neuromuscular junctions (NMJs) were unaltered compared with the wild type (WT). Thus it was assumed that the sec-10 gene might not influence the known ionotropic receptors in the NMJs, but some other pathways instead.