CAJ | 학술논문

运用微生物培养方法,从饭店排污口污泥中筛选了7株产表面活性剂菌株.为了研究产表面活性剂菌在柴油降解过程中所产生的影响,分别将这些菌株与柴油降解菌(实验室提取的另一株假单胞菌)共同作用,其中一株(B_(26))不但可以将降解诱导期从6 d缩短至4 d,而且能将降解率由原来的71.1%提高至80.6%,具有较大的研究价值.实验着重对B_(26)的作用机理、柴油降解的影响因素等进行了分析研究.经形态学和生理生化实验鉴定,B_(26)属于芽孢菌属,其代谢过程中产生脂肽类表面活性剂,利用冷冻干燥法提取生物表面活性剂产量为1.57 g/L.
운용미생물배양방법,종반점배오구오니중사선료7주산표면활성제균주.위료연구산표면활성제균재시유강해과정중소산생적영향,분별장저사균주여시유강해균(실험실제취적령일주가단포균)공동작용,기중일주(B_(26))불단가이장강해유도기종6 d축단지4 d,이차능장강해솔유원래적71.1%제고지80.6%,구유교대적연구개치.실험착중대B_(26)적작용궤리、시유강해적영향인소등진행료분석연구.경형태학화생리생화실험감정,B_(26)속우아포균속,기대사과정중산생지태류표면활성제,이용냉동간조법제취생물표면활성제산량위1.57 g/L.
Seven biosurfactant producing strains, obtained from sewage of a restaurant by method of microbe cultivation, were respectively interacted with a diesel oil degradable bacteria (a kind of Pseudomonas strains obtained also in lab) to study the influences of biosurfactants on diesel oil degradation. The interaction showed that B_(26), one of the seven strains, could not only reduce the degradation induction peoriod from 6 days to 4 days but also increase the degradation rate from 71.1% to 80. 6% , which deserved further research. All tests were focused on analysis of mechanism and the affecting factors in diesel oil degradation. Based on the morphological, physiological and biochemical test results, B_(26) was identified as Bacillus sp. , in whose metabolism lipopeptide biosurfactant could be produced, and 1.57 g/L of them could be extracted using the freeze-drying method.