CAJ | 학술논문

目的探讨微小RNA-21(miR-21)在上皮性卵巢癌组织中的表达及其与临床病理特征间的关系.方法采用茎环实时荧光逆转录聚合酶链反应(real-time RT-PCR)检测48例上皮性卵巢癌组织、24例良性上皮性卵巢肿瘤及15例正常卵巢组织标本中miR-21表达.结果茎环real-time RT-PCR检测miR-21表达的敏感性和特异性良好;miR-21在卵巢癌组织中的相对表达量(4.849±1.813)显著高于良性卵巢肿瘤(1.133±0.291)及正常卵巢组织(P<0.01),后两组间miR-21表达无明显差异.miR-21表达随卵巢癌的临床分期和组织分级的进展呈上升趋势,而且在有淋巴结转移组明显高于无淋巴结转移组(P均<0.01).结论 miR-21在上皮性卵巢癌组织中的表达升高,提示其可能在卵巢癌的发生发展中发挥致癌基因的作用,并且与卵巢癌的演进及不良预后密切相关.
목적 탐토미소RNA-21(miR-21)재상피성란소암조직중적표체급기여림상병리특정간적관계.방법 채용경배실시형광역전록취합매련반응(real-time RT-PCR)검측48례상피성란소암조직、24례량성상피성란소종류급15례정상란소조직표본중miR-21표체.결과 경배real-time RT-PCR검측miR-21표체적민감성화특이성량호;miR-21재란소암조직중적상대표체량(4.849±1.813)현저고우량성란소종류(1.133±0.291)급정상란소조직(P<0.01),후량조간miR-21표체무명현차이.miR-21표체수란소암적림상분기화조직분급적진전정상승추세,이차재유림파결전이조명현고우무림파결전이조(P균<0.01).결론 miR-21재상피성란소암조직중적표체승고,제시기가능재란소암적발생발전중발휘치암기인적작용,병차여란소암적연진급불량예후밀절상관.
Objective To investigate the expression of microRNA-21 (miR-21) in ovarian epithelial carcinoma and its association with the clinicopathological features. Methods The expression of miR-21 was detected by Stem-loop real-time RT-PCR in 48 cases of ovarian epithelial carcinomas, 24 cases of benign ovarian epithelial tumors and 15 cases of normal ovarian tissues. Results The relative expression level of miR-21 (2-~(△△CT)) was 4.849±1.813 in the ovarian epithelial carcinomas, significantly higher than that in the benign ovarian tumors and normal ovarian tissues (P<0.01), but comparable between the latter two groups. The expression of miR-21 was not correlated to the histological type, but increased significantly with the progression of the clinical stages and histological grading (P<0.01), showing a close correlation to lymphatic metastasis. Conclusion MiR-21 might play a role as an oncogene in the tumorigenesis and development of ovarian epithelial carcinoma, and is possibly correlated to the progression and prognosis of ovarian epithelial carcinoma.