环境科学研究
環境科學研究
배경과학연구
RSEARCH OF ENUIRONMENTAL SCIENCES
2010年
3期
355-360
,共6页
刘健楠%汪苹%尹明锐%王磊
劉健楠%汪蘋%尹明銳%王磊
류건남%왕평%윤명예%왕뢰
味精废水脱氮系统%异养硝化-好氧反硝化%分离鉴定%脱氮效率
味精廢水脫氮繫統%異養硝化-好氧反硝化%分離鑒定%脫氮效率
미정폐수탈담계통%이양초화-호양반초화%분리감정%탈담효솔
monosodium glutamate wastewater denitrification system%heterotrophic nitrification-aerobic denitrifier%isolation and identification%nitrogen removal rate
利用BTB培养基快速高效地从某味精厂废水处理系统的活性污泥中筛选出一株高效异养硝化-好氧反硝化细菌LH2-3.通过形态观察,生理生化特征测定,Biolog鉴定以及16S rDNA分子鉴定,认定该菌株是巨大芽孢杆菌(Bacillus megaterium).菌株脱氮性能研究表明:在异养硝化过程中,该菌株可将培养液中的NH_4~+-N 100%去除,对TN的去除率可达98.16%;在好氧反硝化过程中,其对NO_2~-N的去除率达到97.14%,对TN的去除率达到94.97%.
利用BTB培養基快速高效地從某味精廠廢水處理繫統的活性汙泥中篩選齣一株高效異養硝化-好氧反硝化細菌LH2-3.通過形態觀察,生理生化特徵測定,Biolog鑒定以及16S rDNA分子鑒定,認定該菌株是巨大芽孢桿菌(Bacillus megaterium).菌株脫氮性能研究錶明:在異養硝化過程中,該菌株可將培養液中的NH_4~+-N 100%去除,對TN的去除率可達98.16%;在好氧反硝化過程中,其對NO_2~-N的去除率達到97.14%,對TN的去除率達到94.97%.
이용BTB배양기쾌속고효지종모미정엄폐수처리계통적활성오니중사선출일주고효이양초화-호양반초화세균LH2-3.통과형태관찰,생리생화특정측정,Biolog감정이급16S rDNA분자감정,인정해균주시거대아포간균(Bacillus megaterium).균주탈담성능연구표명:재이양초화과정중,해균주가장배양액중적NH_4~+-N 100%거제,대TN적거제솔가체98.16%;재호양반초화과정중,기대NO_2~-N적거제솔체도97.14%,대TN적거제솔체도94.97%.
By using BTB medium,a heterotrophic nitrification-aerobic denitrifier LH2-3 was quickly and efficiently isolated from the activated sludge in an MSG wastewater denitrification system.Through morphological observation,physiological and biochemical features determination,Biolog system analysis and 16S rDNA sequence analysis,it was identified as Bacillus megaterium.The results show that ammonia nitrogen loss through heterotrophic nitrification in the liquid culture medium inoculated with LH2-3 reached 100%,and the removal rate of TN was 98.16%.The removal rate of nitrate by this strain was 97.14%,and the removal rate of TN was 94.97% through aerobic denitrification.
