中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2010年
5期
479-481
,共3页
赵利东%薛连国%王莹%杨晋%朱明清%赵绍林
趙利東%薛連國%王瑩%楊晉%硃明清%趙紹林
조리동%설련국%왕형%양진%주명청%조소림
骨髓增生异常综合征%免疫表型%流式细胞术
骨髓增生異常綜閤徵%免疫錶型%流式細胞術
골수증생이상종합정%면역표형%류식세포술
Myelodysplastic syndrome%Immunophenotyping%Flow cytometry
目的 分析CD38、CD133抗原在骨髓增生异常综合征(MDS)中的表达及临床意义.方法 MDS患者31例,其中难治性贫血(RA)21例,RA伴铁幼粒细胞增多(RAS)1例,RA伴原始粒细胞增多(RAEB)9例;各种贫血患者11例(贫血组);以同期收治血常规一系或二系减少但骨髓象正常的非血液病患者10例为对照组;采用流式细胞术对3组患者CD38、CD133表达情况进行分析.结果 31例MDS患者有18例(58.1%)表达CD38,其中RA 12例,占57.1%(12/21),RAEB 6例,占66.7%(6/9);20例表达CD133(64.5%),其中RA 11例,占52.4%,RAS 1例,RAEB 8例,占88.9%.对照组CD38、CD133阳性表达率均为10.0%(1/10),贫血组分别为9.1%(1/11)和18.2%(2/11).与贫血组比较:RA组CD38阳性表达率高于贫血组,差异有统计学意义(x2=6.91,P=0.01).RAEB组CD38及CD133阳性表达率表达亦高于贫血组,差异有统计学意义(x2值分别为7.21、9.90,P值分别为0.01、0.00).MDS组CD38、CD133阳性表达率显著高于对照组(x2值分别为7.02,8.99,P均<0.05).RA组CD38、CD133阳性表达率亦高于对照组,差异有统计学意义(x2值分别为6.18、5.13,P值分别为0.01、0.02);RAEB组CD38、CD133阳性表达率亦高于对照组,差异均有统计学意义(x2值分别为6.54、11.82,P值分别为0.02、0.00).结论 在常规开展的抗体组合的基础上,利用流式细胞术检测CD38、CD133可提高MDS的诊断率.
目的 分析CD38、CD133抗原在骨髓增生異常綜閤徵(MDS)中的錶達及臨床意義.方法 MDS患者31例,其中難治性貧血(RA)21例,RA伴鐵幼粒細胞增多(RAS)1例,RA伴原始粒細胞增多(RAEB)9例;各種貧血患者11例(貧血組);以同期收治血常規一繫或二繫減少但骨髓象正常的非血液病患者10例為對照組;採用流式細胞術對3組患者CD38、CD133錶達情況進行分析.結果 31例MDS患者有18例(58.1%)錶達CD38,其中RA 12例,佔57.1%(12/21),RAEB 6例,佔66.7%(6/9);20例錶達CD133(64.5%),其中RA 11例,佔52.4%,RAS 1例,RAEB 8例,佔88.9%.對照組CD38、CD133暘性錶達率均為10.0%(1/10),貧血組分彆為9.1%(1/11)和18.2%(2/11).與貧血組比較:RA組CD38暘性錶達率高于貧血組,差異有統計學意義(x2=6.91,P=0.01).RAEB組CD38及CD133暘性錶達率錶達亦高于貧血組,差異有統計學意義(x2值分彆為7.21、9.90,P值分彆為0.01、0.00).MDS組CD38、CD133暘性錶達率顯著高于對照組(x2值分彆為7.02,8.99,P均<0.05).RA組CD38、CD133暘性錶達率亦高于對照組,差異有統計學意義(x2值分彆為6.18、5.13,P值分彆為0.01、0.02);RAEB組CD38、CD133暘性錶達率亦高于對照組,差異均有統計學意義(x2值分彆為6.54、11.82,P值分彆為0.02、0.00).結論 在常規開展的抗體組閤的基礎上,利用流式細胞術檢測CD38、CD133可提高MDS的診斷率.
목적 분석CD38、CD133항원재골수증생이상종합정(MDS)중적표체급림상의의.방법 MDS환자31례,기중난치성빈혈(RA)21례,RA반철유립세포증다(RAS)1례,RA반원시립세포증다(RAEB)9례;각충빈혈환자11례(빈혈조);이동기수치혈상규일계혹이계감소단골수상정상적비혈액병환자10례위대조조;채용류식세포술대3조환자CD38、CD133표체정황진행분석.결과 31례MDS환자유18례(58.1%)표체CD38,기중RA 12례,점57.1%(12/21),RAEB 6례,점66.7%(6/9);20례표체CD133(64.5%),기중RA 11례,점52.4%,RAS 1례,RAEB 8례,점88.9%.대조조CD38、CD133양성표체솔균위10.0%(1/10),빈혈조분별위9.1%(1/11)화18.2%(2/11).여빈혈조비교:RA조CD38양성표체솔고우빈혈조,차이유통계학의의(x2=6.91,P=0.01).RAEB조CD38급CD133양성표체솔표체역고우빈혈조,차이유통계학의의(x2치분별위7.21、9.90,P치분별위0.01、0.00).MDS조CD38、CD133양성표체솔현저고우대조조(x2치분별위7.02,8.99,P균<0.05).RA조CD38、CD133양성표체솔역고우대조조,차이유통계학의의(x2치분별위6.18、5.13,P치분별위0.01、0.02);RAEB조CD38、CD133양성표체솔역고우대조조,차이균유통계학의의(x2치분별위6.54、11.82,P치분별위0.02、0.00).결론 재상규개전적항체조합적기출상,이용류식세포술검측CD38、CD133가제고MDS적진단솔.
Objective Analysis of the expression of CD38,CD133 antigen and their clinical significance in myelodysplastic syndrome (MDS).Methods CD38 and CD133 antigen were analyzed by flow cytometry in 31 cases of MDS patients.Results CD38 was expressed in 18 cases (58.1% ),among them,12 cases were found to be myelodysplastic syndrome refractoryanermia ( MDS-RA ),accounting for 57.1%,6 cases were found to be MDS-RAEB,accounting for 66.7%.CD133 was expressed in 20 cases(64.5% ) ,among them,11 cases were found to be MDS-RA ( 52.4% ),1 case MDS-RAS,and 8 cases of MDS-RAEB,accounting for 88.9% .CD38 expressed significantly higher in MDS than anemia and relatively normal group ( P < 0.05 ).CD133 expression in anemia groups was different from MDS-RA without statistical significance ( P > 0.05 ),but was significantly different from relatively normal group (P <0.05).CD133 expression was significantly higher in these with MDS-RAEB than those in anemia and normal group ( P < 0.05 ).Conclusions Combining with conventional antibodies,flow cytometry used in detection of CD38 ,CD133 ,could improve the diagnostic rate of MDS.