中华核医学与分子影像杂志
中華覈醫學與分子影像雜誌
중화핵의학여분자영상잡지
Chinese Journal of Nuclear Medicine and Molecular Imaging
2012年
1期
16-21
,共6页
卢瞳%文颂%周官辉%居胜红%滕皋军
盧瞳%文頌%週官輝%居勝紅%滕皋軍
로동%문송%주관휘%거성홍%등고군
动脉粥样硬化%磁共振成像%近红外荧光成像%动物,实验
動脈粥樣硬化%磁共振成像%近紅外熒光成像%動物,實驗
동맥죽양경화%자공진성상%근홍외형광성상%동물,실험
Atherosclerosis%Magnetic resonance imaging%Near-infrared fluoroscence imaging%Animals,laboratory
目的 探讨7.0 T MRI和近红外荧光成像(NIRF)检测动脉粥样硬化(AS)斑块的可行性.方法 对14周龄ApoE-/-小鼠按高脂饮食喂养20周,建立AS模型,以正常C57BL/6小鼠作为对照.MRI实验中,5只ApoE-/-小鼠及5只C57小鼠经尾静脉注入超微超顺磁性氧化铁颗粒(USPIO)前及36 h后分别行7.0 T MRI.NIRF实验中,10只ApoE-/-小鼠和4只C57小鼠经尾静脉注入抗氧化修饰的低密度脂蛋白(oxLDL)抗体-NIR 797(抗-oxLDL-抗体-NIR 797)近红外探针,4只ApoE-/-小鼠经尾静脉注入非特异性IgG-NIR 797,另4只ApoE-/-小鼠注入PBS,24h后分别行NIRF.用SPSS17.0软件对计量数据行独立样本t检验和单因素方差分析.结果 ApoE-/-小鼠注入USPIO 36 h后,在T2WI上腹主动脉斑块信号较注射前减低,相对信号强度分别为0.70±0.04和1.28±0.06,差异有统计学意义(t =3.376,P＜O.05),信号改变率达(-56.58±4.25)％;普鲁士蓝染色证实斑块内有铁沉积.注入抗-oxLDL-抗体-NIR 797 24 h后,ApoE-/-小鼠主动脉离体NIRF示强荧光信号(SNR为42.51 ±5.24)聚集于主动脉根、主动脉弓及降主动脉起始段,而非特异性IgG-NIR 797组(19.58±3.06)、PBS组(4.19±0.82)及对照C57小鼠(2.29±1.11)仅见较弱荧光信号,与靶向探针组比较差异有统计学意义(F =25.104,P＜0.05).斑块油红O染色与NIRF阳性面积分别为(41.69 ±5.29)％和(39.45±5.35)％,两者呈线性相关(r=0.738,P＜0.05,n=8),免疫荧光证实斑块内oxLDL的表达与巨噬细胞共区域.结论 应用新型分子影像探针在7.0 T MRI和NIRF上可有效检测AS斑块,有助于鉴别高危斑块,可为AS多模式成像提供依据.
目的 探討7.0 T MRI和近紅外熒光成像(NIRF)檢測動脈粥樣硬化(AS)斑塊的可行性.方法 對14週齡ApoE-/-小鼠按高脂飲食餵養20週,建立AS模型,以正常C57BL/6小鼠作為對照.MRI實驗中,5隻ApoE-/-小鼠及5隻C57小鼠經尾靜脈註入超微超順磁性氧化鐵顆粒(USPIO)前及36 h後分彆行7.0 T MRI.NIRF實驗中,10隻ApoE-/-小鼠和4隻C57小鼠經尾靜脈註入抗氧化脩飾的低密度脂蛋白(oxLDL)抗體-NIR 797(抗-oxLDL-抗體-NIR 797)近紅外探針,4隻ApoE-/-小鼠經尾靜脈註入非特異性IgG-NIR 797,另4隻ApoE-/-小鼠註入PBS,24h後分彆行NIRF.用SPSS17.0軟件對計量數據行獨立樣本t檢驗和單因素方差分析.結果 ApoE-/-小鼠註入USPIO 36 h後,在T2WI上腹主動脈斑塊信號較註射前減低,相對信號彊度分彆為0.70±0.04和1.28±0.06,差異有統計學意義(t =3.376,P＜O.05),信號改變率達(-56.58±4.25)％;普魯士藍染色證實斑塊內有鐵沉積.註入抗-oxLDL-抗體-NIR 797 24 h後,ApoE-/-小鼠主動脈離體NIRF示彊熒光信號(SNR為42.51 ±5.24)聚集于主動脈根、主動脈弓及降主動脈起始段,而非特異性IgG-NIR 797組(19.58±3.06)、PBS組(4.19±0.82)及對照C57小鼠(2.29±1.11)僅見較弱熒光信號,與靶嚮探針組比較差異有統計學意義(F =25.104,P＜0.05).斑塊油紅O染色與NIRF暘性麵積分彆為(41.69 ±5.29)％和(39.45±5.35)％,兩者呈線性相關(r=0.738,P＜0.05,n=8),免疫熒光證實斑塊內oxLDL的錶達與巨噬細胞共區域.結論 應用新型分子影像探針在7.0 T MRI和NIRF上可有效檢測AS斑塊,有助于鑒彆高危斑塊,可為AS多模式成像提供依據.
목적 탐토7.0 T MRI화근홍외형광성상(NIRF)검측동맥죽양경화(AS)반괴적가행성.방법 대14주령ApoE-/-소서안고지음식위양20주,건립AS모형,이정상C57BL/6소서작위대조.MRI실험중,5지ApoE-/-소서급5지C57소서경미정맥주입초미초순자성양화철과립(USPIO)전급36 h후분별행7.0 T MRI.NIRF실험중,10지ApoE-/-소서화4지C57소서경미정맥주입항양화수식적저밀도지단백(oxLDL)항체-NIR 797(항-oxLDL-항체-NIR 797)근홍외탐침,4지ApoE-/-소서경미정맥주입비특이성IgG-NIR 797,령4지ApoE-/-소서주입PBS,24h후분별행NIRF.용SPSS17.0연건대계량수거행독립양본t검험화단인소방차분석.결과 ApoE-/-소서주입USPIO 36 h후,재T2WI상복주동맥반괴신호교주사전감저,상대신호강도분별위0.70±0.04화1.28±0.06,차이유통계학의의(t =3.376,P＜O.05),신호개변솔체(-56.58±4.25)％;보로사람염색증실반괴내유철침적.주입항-oxLDL-항체-NIR 797 24 h후,ApoE-/-소서주동맥리체NIRF시강형광신호(SNR위42.51 ±5.24)취집우주동맥근、주동맥궁급강주동맥기시단,이비특이성IgG-NIR 797조(19.58±3.06)、PBS조(4.19±0.82)급대조C57소서(2.29±1.11)부견교약형광신호,여파향탐침조비교차이유통계학의의(F =25.104,P＜0.05).반괴유홍O염색여NIRF양성면적분별위(41.69 ±5.29)％화(39.45±5.35)％,량자정선성상관(r=0.738,P＜0.05,n=8),면역형광증실반괴내oxLDL적표체여거서세포공구역.결론 응용신형분자영상탐침재7.0 T MRI화NIRF상가유효검측AS반괴,유조우감별고위반괴,가위AS다모식성상제공의거.
Objective To explore the feasibility of detecting atherosclerotic plaques with 7.0 T MRI and near-infrared fluorescence imaging(NIRF) using molecular imaging probes.Methods Atherosclerotic plaques were established in male atherosclerotic apolipoprotein E knockout(ApoE-/-) mice fed with high-cholesterol diet for 20 weeks.Wild-type C57BL/6 mice were used as negative controls.7.0 T MRI was performed before and 36 h after intravenously administration of ultrasmall superparamagnetic partical of iron oxide (USPIO).NIR 797 was conjugated with anti-mouse-oxidized modified low density lipoprotein (oxLDL) antibodies to construct an anti-oxLDL-Ab-NIR 797 probe while non-specific IgG-NIR 797 and PBS used as controls.NIRF was performed 24 h after tail vein injection of the probe.Independent sample ttest and one-way analysis of variance were used to analyze the data by SPSS 17.O.Results In APOE-/-mice,in vivo 36 h post-USPIO T2WI images revealed strong focal signal loss in the abdominal aorta than that of pre-USPIO,with relative signal intensity 0.70 ± 0.04 and 1.28 ± 0.06,respectively ( t =3.376,P ＜0.05 ).The percent of signal reduced was ( - 56.58 ± 4.25 ) ％.The Prussian blue staining confirmed the depositions of iron particles in the plaque lesions.Significant fluorochrome accumulation in atherosclerotic plaques was demonstrated in aortic root,aortic arch and the starting of descending aorta 24 h after injection of the anti-oxLDL-Ab-NIR 797 probe.Minimal antibody uptake was observed in normal vessels from wildtype mice receiving the anti-oxLDL-Ab-NIR 797 (SNR:2.29 ± 1.11 ) and in atherosclerotic vessels from ApoE-/- mice receiving the non-specific IgG-NIR 797 ( 19.58 ±3.06) or PBS (4.19 ±0.82),which was significantly different from the uptake of anti-oxLDL-Ab-NIR 797 group (42.51 ±5.24,F =25.104,P ＜ 0.05 ).Comparison between oil red O staining and NIRF 24 h after injection of NIR 797 labeled oxLDL-antibody revealed a significant correlation ( r =0.738,P ＜ 0.05,n =8 ).The positive areas in imaging were (41.69 ± 5.29) ％ and (39.45 ± 5.35 ) ％,respectively.Immunofluorescence staining demonstrated that the expression of oxLDL was closely associated to macrophage infiltrates.Conclusion This study demonstrates that atherosclerotic plaque MRI and NIRF imaging are feasible by using novel molecular imaging probes and may help to identify high-risk plaques,providing a foundation for multimodality imaging of atherosclerosis.
