CAJ | 학술논문

目的对去细胞猪主动脉瓣膜进行聚乙二醇(PEG)化改性,并评价改性后复合支架的细胞毒性.方法合成功能基团为丙烯酰基的枝化状聚乙二醇衍生物,在去细胞猪主动脉瓣膜引入巯基,通过迈克尔加成反应完成PEG对去细胞猪主动脉瓣膜的交联,并作PEG改性前后瓣膜的生物力学测定.制作去细胞瓣膜的浸提液,浸提液分为单纯去细胞瓣膜组、PEG改性去细胞瓣膜组和阴性对照组(培养液),采用CCK-8法检测复合支架的浸提液对人脐静脉内皮细胞增殖率的影响,评价其细胞毒性.结果 PEG改性去细胞瓣膜反应条件温和,改性效果确切.复合瓣膜支架的抗拉强度明显高于去细胞瓣膜[(7.53±0.29)MPa比(5.65±0.24)MPa,P＜0.05],但与天然瓣膜[(7.68±0.20)MPa]差异无统计学意义(P＞0.05).复合支架和去细胞瓣膜毒性评级均0级,与阴性对照组差异无统计学意义(P＞0.05).人脐静脉内皮细胞经各浸提液的培养液培养后形态良好,增殖旺盛.结论 PEG改性能明显改善去细胞猪主动脉瓣膜的力学性能,且改性后的复合支架无细胞毒性,为进一步研究提供依据.
목적 대거세포저주동맥판막진행취을이순(PEG)화개성,병평개개성후복합지가적세포독성.방법 합성공능기단위병희선기적지화상취을이순연생물,재거세포저주동맥판막인입구기,통과매극이가성반응완성PEG대거세포저주동맥판막적교련,병작PEG개성전후판막적생물역학측정.제작거세포판막적침제액,침제액분위단순거세포판막조、PEG개성거세포판막조화음성대조조(배양액),채용CCK-8법검측복합지가적침제액대인제정맥내피세포증식솔적영향,평개기세포독성.결과 PEG개성거세포판막반응조건온화,개성효과학절.복합판막지가적항랍강도명현고우거세포판막[(7.53±0.29)MPa비(5.65±0.24)MPa,P＜0.05],단여천연판막[(7.68±0.20)MPa]차이무통계학의의(P＞0.05).복합지가화거세포판막독성평급균0급,여음성대조조차이무통계학의의(P＞0.05).인제정맥내피세포경각침제액적배양액배양후형태량호,증식왕성.결론 PEG개성능명현개선거세포저주동맥판막적역학성능,차개성후적복합지가무세포독성,위진일보연구제공의거.
Objective To modify acellular porcine aortic valve scaffolds using polyethylene glycol (PEG) and to assess their cytotoxicity in vitro. Methods Dendritic PEG derivatives with acryloyl functional group were synthesized followed by introduction of thiol groups into acellular porcine aortic valves and cross- linking with PEG via Michael addition reaction.The biomechanical properties prior to and after PEG modification were compared.The extracts were divided into three groups:decellularized valve group,PEG - modified decellularized valve group, and control group (RPMI 1640 medium ).The impact of composite scaffolds extractant on human umbilical vein endothelial cells proliferation and in vitro cytotoxicity were assessed based on CCK - 8 assay.Results Modification of acellular porcine aortic valves using PEG occurred under mild reaction conditions and showed well-defined outcomes.Composite valve scaffolds had a markedly higher stretch- proof capacity than acellular porcine aortic valves [ (7.53±0.29) MPa vs (5.65±0.24) MPa,P＜0.05] but did not differ statistically from natural valves [ (7.53 ±0.29)MPa,P＞0.05].Cytotoxicity of composite scaffolds and acellular porcine valves were both graded as zero.Human umbilical vein endothelial cells cultured with medium containing composite valve scaffold extractants appeared in normal morphology and active proliferation.Conclusion PEG modification significantly improves the mechanical properties of acellular porcine aortic valves and reduces the cytotoxicity of composite scaffolds,thereby providing prospects for further researches.