中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2012年
7期
421-424
,共4页
刘映霞%杨桂林%刘琅妮%刘敏%胡国龄
劉映霞%楊桂林%劉瑯妮%劉敏%鬍國齡
류영하%양계림%류랑니%류민%호국령
乙型肝炎%杀伤细胞,天然%受体,免疫%DNA,病毒
乙型肝炎%殺傷細胞,天然%受體,免疫%DNA,病毒
을형간염%살상세포,천연%수체,면역%DNA,병독
Hepatitis B%Killer cells,natural%Receptors,immunologic%DNA,viral
目的 探讨乙型肝炎患者自然杀伤(NK)细胞表面NKG2D受体差异表达及临床意义.方法 应用流式细胞术和RT-PCR方法检测慢性乙型肝炎(CHB)患者22例、急性乙型肝炎(AHB)患者18例、HBV携带者(HBVC) 10例以及18名健康对照者(HD)的外周血NK细胞NKG2D蛋白和NKG2D mRNA表达水平,分析NKG2D表达水平与血清中HBV DNA定量的相互关系.采用方差分析和直线回归进行统计学处理.结果 NKG2D mRNA表达水平HBVC组为0.96±0.17、HD组为1.03±0.12、AHB组为1.53±0.30、CHB组为1.51±0.35,各组间的差异有统计学意义(q=7.586、7.485、7.920、7.880,均P＜0.01).NK细胞中NKG2D蛋白表达量AHB组为0.87±0.14、HD组为0.89±0.17、CHB组为0.67±0.09、HBVC组为0.59±0.13,各组间的差异有统计学意义(q=6.92、7.67、7.53、8.16,均P＜0.01).乙型肝炎患者NK细胞NKG2DmRNA表达水平与其对应的血清中HBV DNA水平分别呈负相关(CHB组r=-0.75,P＜0.01；AHB组r=-0.66,P＜0.01；HBVC组r=-0.69,P＜0.01).AHB、CHB NK细胞NKG2D蛋白表达量与血清HBV DNA水平呈负相关(r=-0.47、-0.45,均P＜0.05).结论 NKG2D诱导的NK细胞毒可能在乙型肝炎HBV清除中起一定的作用.
目的 探討乙型肝炎患者自然殺傷(NK)細胞錶麵NKG2D受體差異錶達及臨床意義.方法 應用流式細胞術和RT-PCR方法檢測慢性乙型肝炎(CHB)患者22例、急性乙型肝炎(AHB)患者18例、HBV攜帶者(HBVC) 10例以及18名健康對照者(HD)的外週血NK細胞NKG2D蛋白和NKG2D mRNA錶達水平,分析NKG2D錶達水平與血清中HBV DNA定量的相互關繫.採用方差分析和直線迴歸進行統計學處理.結果 NKG2D mRNA錶達水平HBVC組為0.96±0.17、HD組為1.03±0.12、AHB組為1.53±0.30、CHB組為1.51±0.35,各組間的差異有統計學意義(q=7.586、7.485、7.920、7.880,均P＜0.01).NK細胞中NKG2D蛋白錶達量AHB組為0.87±0.14、HD組為0.89±0.17、CHB組為0.67±0.09、HBVC組為0.59±0.13,各組間的差異有統計學意義(q=6.92、7.67、7.53、8.16,均P＜0.01).乙型肝炎患者NK細胞NKG2DmRNA錶達水平與其對應的血清中HBV DNA水平分彆呈負相關(CHB組r=-0.75,P＜0.01；AHB組r=-0.66,P＜0.01；HBVC組r=-0.69,P＜0.01).AHB、CHB NK細胞NKG2D蛋白錶達量與血清HBV DNA水平呈負相關(r=-0.47、-0.45,均P＜0.05).結論 NKG2D誘導的NK細胞毒可能在乙型肝炎HBV清除中起一定的作用.
목적 탐토을형간염환자자연살상(NK)세포표면NKG2D수체차이표체급림상의의.방법 응용류식세포술화RT-PCR방법검측만성을형간염(CHB)환자22례、급성을형간염(AHB)환자18례、HBV휴대자(HBVC) 10례이급18명건강대조자(HD)적외주혈NK세포NKG2D단백화NKG2D mRNA표체수평,분석NKG2D표체수평여혈청중HBV DNA정량적상호관계.채용방차분석화직선회귀진행통계학처리.결과 NKG2D mRNA표체수평HBVC조위0.96±0.17、HD조위1.03±0.12、AHB조위1.53±0.30、CHB조위1.51±0.35,각조간적차이유통계학의의(q=7.586、7.485、7.920、7.880,균P＜0.01).NK세포중NKG2D단백표체량AHB조위0.87±0.14、HD조위0.89±0.17、CHB조위0.67±0.09、HBVC조위0.59±0.13,각조간적차이유통계학의의(q=6.92、7.67、7.53、8.16,균P＜0.01).을형간염환자NK세포NKG2DmRNA표체수평여기대응적혈청중HBV DNA수평분별정부상관(CHB조r=-0.75,P＜0.01；AHB조r=-0.66,P＜0.01；HBVC조r=-0.69,P＜0.01).AHB、CHB NK세포NKG2D단백표체량여혈청HBV DNA수평정부상관(r=-0.47、-0.45,균P＜0.05).결론 NKG2D유도적NK세포독가능재을형간염HBV청제중기일정적작용.
Objective To investigate difference of NKG2D receptor expression level on the surface of natural killer (NK) cells in the patients with hepatitis B and its clinical significance.Methods This was a four-arm study with different types of subjects,including patients with chronic hepatitis B (CHB,n =22),HBV carriers (HBVC,n=10),patients with acute hepatitis B (AHB,n=18) and healthy donors (HD,n=18).NKG2D protein and mRNA levels on the surface NK cells in the peripheral blood were examined by reverse transcription-polymerase chain reaction assay.The relationship between NKG 2D expression and serum hepatitis B virus (HBV) DNA level was analyzed.The data were compared by analysis of variance and linear regression.Results NKG2D mRNA expression levels in groups of HBVC, HD, AHB and CHB were 0.96±0.17, 1.03±0.12,1.53±0.30 and 1.51 ± 0.35,respectively; the differences among groups were statistically significant (q=7.586,7.485,7.920 and 7.880,respectively; all P＜0.01).NKG2D protein expression levels in groups of AHB,HD,CHB and HBVC were 0.87±0.14,0.89±0.17,0.67±0.09 and 0.59±0.13,respectively; the differences among groups were statistically significant (q=6.92,7.67,7.53and 8.16,respectively; all P＜0.01).The NKG2D mRNA expression levels on NK cells were negatively correlated with serum HBV DNA viral loads in patients with CHB,AHB or HBVC (r=-0.75,-0.66 and-0.69,respectively; all P＜0.01).The NKG2D protein levels on NK cells from patients with AHB and CHB were negatively correlated with serum HBV DNA levels (r=-0.47 and -0.45,respectively; both P＜0.05).Conclusion NKG2D mediated NK cytotoxicity may play a role in viral clearance in hepatitis B.
