中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2008年
10期
1303-1306
,共4页
高缨%褚红女%郭春娟%沈美娟%吕小玲%侯宇宁%王金福
高纓%褚紅女%郭春娟%瀋美娟%呂小玲%侯宇寧%王金福
고영%저홍녀%곽춘연%침미연%려소령%후우저%왕금복
骨髓细胞/细胞学%间质干细胞/细胞学%细胞培养%造血干细胞%细胞增殖
骨髓細胞/細胞學%間質榦細胞/細胞學%細胞培養%造血榦細胞%細胞增殖
골수세포/세포학%간질간세포/세포학%세포배양%조혈간세포%세포증식
Bone marrow cells/CY%Mesenchymal stem cells/CY%Cell culture%Hematopoietic stem cells%Cell proliferation
目的 体外分离培养人新鲜脐血(UCB)造血干细胞(HSC),利用骨髓问充质干细胞作为滋养层联合细胞因子组合以扩增脐血造血干/祖细胞.方法 采用Ficoll淋巴细胞分离液密度梯度离心法分离UCBHSC,加入骨髓问充质干细胞和细胞因子,检测脐血造血干/祖细胞总有核细胞(NC)总数和CD34+细胞数.结果 有核细胞总数扩增(75.2±15.0)倍,CD34+细胞数扩增(18.7±12.3)倍.结论 体外HSC培养,加入骨髓问充质干细胞和细胞因子对造血干/祖细胞增殖有明显的作用.
目的 體外分離培養人新鮮臍血(UCB)造血榦細胞(HSC),利用骨髓問充質榦細胞作為滋養層聯閤細胞因子組閤以擴增臍血造血榦/祖細胞.方法 採用Ficoll淋巴細胞分離液密度梯度離心法分離UCBHSC,加入骨髓問充質榦細胞和細胞因子,檢測臍血造血榦/祖細胞總有覈細胞(NC)總數和CD34+細胞數.結果 有覈細胞總數擴增(75.2±15.0)倍,CD34+細胞數擴增(18.7±12.3)倍.結論 體外HSC培養,加入骨髓問充質榦細胞和細胞因子對造血榦/祖細胞增殖有明顯的作用.
목적 체외분리배양인신선제혈(UCB)조혈간세포(HSC),이용골수문충질간세포작위자양층연합세포인자조합이확증제혈조혈간/조세포.방법 채용Ficoll림파세포분리액밀도제도리심법분리UCBHSC,가입골수문충질간세포화세포인자,검측제혈조혈간/조세포총유핵세포(NC)총수화CD34+세포수.결과 유핵세포총수확증(75.2±15.0)배,CD34+세포수확증(18.7±12.3)배.결론 체외HSC배양,가입골수문충질간세포화세포인자대조혈간/조세포증식유명현적작용.
Objective To separate and cultivate homo umbilical cord blood (UCB) hematopoietic stem cell (HSC) in vitro, and u-tilize bone marrow desmohemoblast stem cell as trophoblastic layer combined with cytokine to amplify umbilical cord blood hematopoietic stem/progenitor cell. Methods Ficoll lymph-cell separating medium density gradient centrifugalization was used to segregate UCBHSC.Bone marrow desmohemoblast stem ceil and cytokine were added, and the sum of NC cells and CD34 + cells was counted. Results The sum of NC cells amplified 75.2±15.0 times, and the sum of CD 34 + cells amplified 18.7±12.3 times. Conclusions It has significant effect on amplification of hematopoietic stem cell with bone marrow desmohemoblast stem cell and eytokine when HSC are cultured in vitro.
