哈尔滨医科大学学报
哈爾濱醫科大學學報
합이빈의과대학학보
JOURNAL OF HARBIN MEDICAL UNIVERSITY
2001年
1期
37-38
,共2页
刘艳%杜智敏%姜宝红%高焕焕%艾敬
劉豔%杜智敏%薑寶紅%高煥煥%艾敬
류염%두지민%강보홍%고환환%애경
抗肿瘤药%中药提取物9901%HeLa细胞%体外
抗腫瘤藥%中藥提取物9901%HeLa細胞%體外
항종류약%중약제취물9901%HeLa세포%체외
目的 用体外培养法研究中药提取物9901对肿瘤HeLa细胞生长的抑制作用和作用机制。方法 采用MTT法和激光扫描共聚焦显微镜进行研究。结果 HeLa细胞在9901处理3天后，测得该药的IC50为5.12μg/ml。用不同浓度的9901处理HeLa细胞48h后，用PI染色，HeLa细胞核有形态变化，DNA含量明显减少，经统计学处理，与对照组间有显著性差异。结论 中药提取物9901可有效抑制HeLa细胞的生长，通过影响细胞的DNA而发挥作用。
目的 用體外培養法研究中藥提取物9901對腫瘤HeLa細胞生長的抑製作用和作用機製。方法 採用MTT法和激光掃描共聚焦顯微鏡進行研究。結果 HeLa細胞在9901處理3天後，測得該藥的IC50為5.12μg/ml。用不同濃度的9901處理HeLa細胞48h後，用PI染色，HeLa細胞覈有形態變化，DNA含量明顯減少，經統計學處理，與對照組間有顯著性差異。結論 中藥提取物9901可有效抑製HeLa細胞的生長，通過影響細胞的DNA而髮揮作用。
목적 용체외배양법연구중약제취물9901대종류HeLa세포생장적억제작용화작용궤제。방법 채용MTT법화격광소묘공취초현미경진행연구。결과 HeLa세포재9901처리3천후，측득해약적IC50위5.12μg/ml。용불동농도적9901처리HeLa세포48h후，용PI염색，HeLa세포핵유형태변화，DNA함량명현감소，경통계학처리，여대조조간유현저성차이。결론 중약제취물9901가유효억제HeLa세포적생장，통과영향세포적DNA이발휘작용。
Objective To study the effect of TCD(traditional Chinesedrug)-extract-9901 on growth inhibition in HeLa cancer cell and the action mechanism of drug.Methods The parameters were got by MTT assay and laser scanning confocal microscope.Results The IC50 of this extract was 5.12μg/ml after HeLa cell was treated with 9901 for 3 days.The HeLa cell nucleus with PI staining varied and the DNA contents decreased significantly after HeLa cell were treated with different concentration 9901 for 48 hours.With the statistics test,the DNA contents in treated and control goups were obviously different.Conclusion TCD-extract-9901 can effectively inhibit growth of HeLa cancer cell.The action mechanism of the herb's extract 9901 on HeLa cancer cell may be related with the DNA content in HeLa cell.
