蚕业科学
蠶業科學
잠업과학
ACTA SERICOLOGICA SINICA
2005年
4期
449-453
,共5页
段家龙%佐藤秀对%原三郎
段傢龍%佐籐秀對%原三郎
단가룡%좌등수대%원삼랑
人体醛缩酶C%基因表达%杂交重组病毒%家蚕
人體醛縮酶C%基因錶達%雜交重組病毒%傢蠶
인체철축매C%기인표체%잡교중조병독%가잠
Human FBP aldolase C%Gene expression%HyNPV%Bombyx mori
利用人体醛缩酶C cNDA的一对特异性引物Ald-5'(5'-ggatcccctcactcgtaccca-3')和Ald-3'(5'-ggatcctcagtaggcatggtt-3'),从人脑cDNA文库中PCR扩增出人体醛缩酶C cDNA,并依次被克隆进克隆载体pCR2.1、转移载体pAcGP67B以及来源于AcNPV和BmNPV的杂交重组病毒HyNPV.通过对家蚕血液的SDS-PAGE电泳和酶活力测定,证明人体醛缩酶C cDNA在家蚕体内成功得到表达.
利用人體醛縮酶C cNDA的一對特異性引物Ald-5'(5'-ggatcccctcactcgtaccca-3')和Ald-3'(5'-ggatcctcagtaggcatggtt-3'),從人腦cDNA文庫中PCR擴增齣人體醛縮酶C cDNA,併依次被剋隆進剋隆載體pCR2.1、轉移載體pAcGP67B以及來源于AcNPV和BmNPV的雜交重組病毒HyNPV.通過對傢蠶血液的SDS-PAGE電泳和酶活力測定,證明人體醛縮酶C cDNA在傢蠶體內成功得到錶達.
이용인체철축매C cNDA적일대특이성인물Ald-5'(5'-ggatcccctcactcgtaccca-3')화Ald-3'(5'-ggatcctcagtaggcatggtt-3'),종인뇌cDNA문고중PCR확증출인체철축매C cDNA,병의차피극륭진극륭재체pCR2.1、전이재체pAcGP67B이급래원우AcNPV화BmNPV적잡교중조병독HyNPV.통과대가잠혈액적SDS-PAGE전영화매활력측정,증명인체철축매C cDNA재가잠체내성공득도표체.
Fructose-1,6-bisphosphate (FBP) aldolase C cDNA from human brain cDNA library was amplified via PCR with primers of Aid-5' (5'-ggatcccctcactcgtaccca-3') and Aid-3' (5'-ggatcctcagtaggcatggtt-3')and was successively cloned into cloning vector pCR2.1, transfer vector pAcGP67B, and baculovirus HyNPV (hybrid baculovirus of Autographa californica nuclear polyhedrosis virus and Bombyx mori nuclear polyhedrosis virus). The SDS-PAGE and enzyme activity analyses indicated that recombinant intact human Aldolase C protein was successfully expressed in silkworm larval blood via recombinant virus HyNPV-hAld-c.
