湖南农业大学学报（自然科学版）
湖南農業大學學報（自然科學版）
호남농업대학학보（자연과학판）
JOURNAL OF HUNAN AGRICULTURAL UNIVERSITY(NATURAL SCIENCES)
2009年
3期
237-241
,共5页
富硒酵母%发酵条件%优化
富硒酵母%髮酵條件%優化
부서효모%발효조건%우화
selenium-enriched yeast%cultivation conditions%optimization
对1株经过DES(硫酸二乙酯)、紫外线和氯化锂复合诱变后的产朊假丝酵母进行富硒发酵条件的研究,通过单因素及正交试验,确定葡萄糖为培养基的最佳碳源,添加4%～5%时可得到最高生物量与硒含量;蛋白胨和尿素的复合氮源为最佳氮源.分别添加蛋白胨0.5%,尿素0.9%,辅助氮源酵母膏0.4%,硒 25 μg /mL,摇瓶(250 mL)装液量30 mL,转速200 r/min,培养温度 28 ℃,pH 5～6,培养时间40 h,此条件下酵母生物量达到7.942 g/L,总硒含量达到16 486.48 μg /L.
對1株經過DES(硫痠二乙酯)、紫外線和氯化鋰複閤誘變後的產朊假絲酵母進行富硒髮酵條件的研究,通過單因素及正交試驗,確定葡萄糖為培養基的最佳碳源,添加4%～5%時可得到最高生物量與硒含量;蛋白胨和尿素的複閤氮源為最佳氮源.分彆添加蛋白胨0.5%,尿素0.9%,輔助氮源酵母膏0.4%,硒 25 μg /mL,搖瓶(250 mL)裝液量30 mL,轉速200 r/min,培養溫度 28 ℃,pH 5～6,培養時間40 h,此條件下酵母生物量達到7.942 g/L,總硒含量達到16 486.48 μg /L.
대1주경과DES(류산이을지)、자외선화록화리복합유변후적산원가사효모진행부서발효조건적연구,통과단인소급정교시험,학정포도당위배양기적최가탄원,첨가4%～5%시가득도최고생물량여서함량;단백동화뇨소적복합담원위최가담원.분별첨가단백동0.5%,뇨소0.9%,보조담원효모고0.4%,서 25 μg /mL,요병(250 mL)장액량30 mL,전속200 r/min,배양온도 28 ℃,pH 5～6,배양시간40 h,차조건하효모생물량체도7.942 g/L,총서함량체도16 486.48 μg /L.
The study was made of fermentation conditions of selenium-enriched yeast. The following results were obtained by Single factor and orthogonal tests: the glucose was the optimal carbon source for yeast growth, and the optimal concentration of glucose was 4%～5%. The compound nitrogen which contained urea and peptone was the optimal nitrogen source for fermentation,and the optimal concentration of compound nitrogen were peptone 0.5% and urea 0.9%.The optimal concentration of yeast extract was 0.4%,and the optimal concentration of selenium was 25 μg/mL;the liquid volume of 30 mL in 250 mL flask at the speed of 200 r/min was the optimal condition for fermentation. The optimal initial pH for the strain growth was about 5～6. The optimal cultivation temperature was 28 ℃,and the fermentation could be finished in forty hours. Under the optimized conditions,the biomass (dry weight) reached 7.942 g/L and the total Se content of the cells was 16 486.48 μg /L.
