CAJ | 학술논문

为了解新疆乌苏泥火山细菌多样性,从泥火山泥浆样品中直接提取总DNA,构建了含150个有效转化子的泥火山细菌16S rDNA基因文库,转化子经菌液PCR及HaeⅢ酶切后获得16个不同带型,克隆测序结果表明,其分属于16个不同的分类单元.一部分序列与已知细菌类群的16S rDNA序列相似性较高,归属变形菌门(Proteobacteria),厚壁菌门(Firmicutes),梭杆菌门(Fusobacteria),放线菌门(Actinobacteria);另外一部分序列与已知细菌类群的16S rDNA序列同源性较低,可能代表新的分类单位.研究结果显示,泥火山环境中微生物种群丰富,值得进一步研究.
위료해신강오소니화산세균다양성,종니화산니장양품중직접제취총DNA,구건료함150개유효전화자적니화산세균16S rDNA기인문고,전화자경균액PCR급HaeⅢ매절후획득16개불동대형,극륭측서결과표명,기분속우16개불동적분류단원.일부분서렬여이지세균류군적16S rDNA서렬상사성교고,귀속변형균문(Proteobacteria),후벽균문(Firmicutes),사간균문(Fusobacteria),방선균문(Actinobacteria);령외일부분서렬여이지세균류군적16S rDNA서렬동원성교저,가능대표신적분류단위.연구결과현시,니화산배경중미생물충군봉부,치득진일보연구.
In this study, the mixed environmental genomic DNA was isolated directly from the mud volcano soil in Usu county of Xinjiang. The 16S rRNA genes were amplified with bacterial universal primers and cloned into the pGEM-T Easy Vector to construct a16S rRNA clone library.The total 150 clones were selected by amplified rDNA restriction analysis(ARDRA) using restriction enzymes Hae Ⅲ. Of these, 16 restriction fragment length polymorphism(RFLP)types were used for sequencing. A part of these sequences were closely related to the phylum: Proteobacteria,Firmicutes,Fusobacteria and Actinobacteria (>97% sequence similarity), while another portion showed less affiliation with known taxa (<97% sequence similarity) and might represent novel taxa. The results indicated that the mud volcano in Usu county of Xinjiang is rich in microbial species,which is worth further studying.