CAJ | 학술논문

目的:探讨熊果酸(UA)对卵巢癌细胞株SKOV3及卵巢癌皮下移植瘤生长的抑制作用并探讨其机制.方法:采用四甲基偶氮唑蓝(MTT)比色法,检测UA对SKOV3细胞的增殖抑制效应;建立卵巢癌皮下移植瘤模型,观察UA 对移植瘤的生长抑制作用;用免疫组化检测凋亡相关蛋白Bcl-2和Bax的表达.结果: UA对体外培养的SKOV3细胞生长具有抑制作用,与DDP联合应用使SKOV3细胞生长进一步受到抑制.体内实验表明:各治疗组肿瘤的生长明显受到抑制,而联合治疗组抗瘤作用进一步增强.UA能下调肿瘤细胞中Bcl-2蛋白的表达,同时明显提高肿瘤细胞中Bax蛋白的表达.结论:UA可明显抑制卵巢癌细胞生长,并诱导细胞凋亡,其主要机制与调节Bcl-2和Bax的蛋白表达有关.
목적:탐토웅과산(UA)대란소암세포주SKOV3급란소암피하이식류생장적억제작용병탐토기궤제.방법:채용사갑기우담서람(MTT)비색법,검측UA대SKOV3세포적증식억제효응;건립란소암피하이식류모형,관찰UA 대이식류적생장억제작용;용면역조화검측조망상관단백Bcl-2화Bax적표체.결과: UA대체외배양적SKOV3세포생장구유억제작용,여DDP연합응용사SKOV3세포생장진일보수도억제.체내실험표명:각치료조종류적생장명현수도억제,이연합치료조항류작용진일보증강.UA능하조종류세포중Bcl-2단백적표체,동시명현제고종류세포중Bax단백적표체.결론:UA가명현억제란소암세포생장,병유도세포조망,기주요궤제여조절Bcl-2화Bax적단백표체유관.
Objective:To study the effects of ursolic acid(UA) on ovarian cancer growth and its mechanism. Methods: The proliferation inhibition was examined by MTT assay.Human ovarian cancer cell line SKOV3 was inoculated subcutaneously into nude mice,to observe the effects of ursolic acid on ovarian cancer growth.The levels of Bal-2 and Bax of the ovarian cancer cells were assayed by immunohistochemistry. Results: UA significantly inhibited the proliferation of SKOV3 cells in vitro.UA in combination with DDP might produce a remarkbale inhibitory effect on SKOV3 cells.In vivo,the average weight and the volume of ovarian cancer in UA group was lower than those of control group(P<0.05). Conclusion:UA suppressed the growth of SKOV3 cells and human ovarian cancer exnografts and induce the apoptosis of SKOV3 cells,which may be related with regulating the expression levels of Bal-2 and Bax.