CAJ | 학술논문

为研究异黑成熟颗粒对环磷酰胺诱导免疫抑制小鼠的非特异性免疫功能、体液免疫功能、细胞免疫功能的影响,将84只小鼠随机分为6组:正常对照组,环磷酰胺模型组,阳性对照组,异黑成熟颗粒低、中、高剂量组,每组14只.除正常对照组外,其他组小鼠均用75mg/kg环磷酰胺腹腔注射诱导免疫抑制小鼠模型,阳性对照组和异黑成熟颗粒组连续灌胃给药14d,1次/d.测定小鼠单核巨噬细胞吞噬功能、外周血T细胞亚群、NK细胞活性、白介素-1(IL-1)活性、白介素-2(IL-2)活性、肿瘤坏死因子-α(TNF-α)活性、血清溶血素抗体生成(CH_(50))、迟发型变态反应等.结果表明,异黑成熟颗粒3个剂量组对环磷酰胺诱导的免疫抑制小鼠的单核巨噬细胞吞噬功能、外周血T细胞亚群数量、对2,4-二硝基氯苯(DNCB)诱导的迟发性变态反应、免疫器官重量和IgM、IgG值等均具有明显增强作用(P＜0.05);异黑成熟颗粒中、高剂量组明显增强小鼠NK细胞和TNF-α活性(P＜0.05);异黑成熟颗粒能提高小鼠IL-1和IL-2的活性(P＜0.05).异黑成熟颗粒对免疫抑制小鼠的免疫功能具有改善作用.
위연구이흑성숙과립대배린선알유도면역억제소서적비특이성면역공능、체액면역공능、세포면역공능적영향,장84지소서수궤분위6조:정상대조조,배린선알모형조,양성대조조,이흑성숙과립저、중、고제량조,매조14지.제정상대조조외,기타조소서균용75mg/kg배린선알복강주사유도면역억제소서모형,양성대조조화이흑성숙과립조련속관위급약14d,1차/d.측정소서단핵거서세포탄서공능、외주혈T세포아군、NK세포활성、백개소-1(IL-1)활성、백개소-2(IL-2)활성、종류배사인자-α(TNF-α)활성、혈청용혈소항체생성(CH_(50))、지발형변태반응등.결과표명,이흑성숙과립3개제량조대배린선알유도적면역억제소서적단핵거서세포탄서공능、외주혈T세포아군수량、대2,4-이초기록분(DNCB)유도적지발성변태반응、면역기관중량화IgM、IgG치등균구유명현증강작용(P＜0.05);이흑성숙과립중、고제량조명현증강소서NK세포화TNF-α활성(P＜0.05);이흑성숙과립능제고소서IL-1화IL-2적활성(P＜0.05).이흑성숙과립대면역억제소서적면역공능구유개선작용.
This paper investigates the effects of abnormal savda munziq granular on nonspecific immunity,humeral immunity,cell immunity functions of cyclophosphamide induced immune suppressed mice.84 qualified mice were randomly divided into 6 groups with 14 in each group:normal control group,model group,groups of abnormal savda munziq granular for high,medium,low dosages and the control group.Except for the normal control group,mice in all other groups were made immune suppression models by using cyclophosphamide (75mg/kg,OD).Abnormal savda munziq granular was orally administered for 14 days,once a day.The mononuclear phagocyte englobement function,the quantity of T cell subpopulation in peripheral blood,NK cyto-active,interleukin-1 and interleukin-2 activity,TNF-α activity,serum hemolysin (CH_(50)),delayed allergy were monitored.Three groups of abnormal savda munziq granular were found to have made remarkable improvement in the mononuclear phagocyte englobement function,the quantity of T cell subpopulation in peripheral blood,serum hemolysin,delayed allergy (P＜0.05).The IgM and IgG values were also improved remarkably (P＜0.05).In the groups of abnormal savda munziq granular,the NK cyto-active,and TNF-α,activities were remarkably improved for the medium dose and the high dose groups.There was a tendency in abnormal savda munziq granular groups of increasing the activities of IL-1 in lower dose group and those of IL-2 in medium dose group (P＜0.05).Abnormal savda munziq granular could improve the immunological function of immune suppressed mice.