CAJ | 학술논문

本试验旨在研究十二指肠灌注大豆小肽对奶山羊小肠小肽和游离氨基酸吸收的影响.选择7只体况良好、体质量相近的奶山羊((37.88±3.03)kg),安装永久性十二指肠近端瘘管和门静脉、肠系膜静脉近端和远端以及颈动脉慢性血插管进行4×4拉丁方试验,分别从十二指肠灌注生理盐水、60、120、180 g·d~(-1)大豆小肽.结果表明,随着十二指肠大豆小肽灌注水平的提高,奶山羊肠系膜排流组织(MDV)总肽结合氨基酸净流量显著增加(P<0.05或P<0.01);60、120、180 g·d~(-1)组门静脉排流组织(PDV)总肽结合氨基酸净流量均显著高于对照组(P<0.05),但3个大豆小肽灌注组间无显著性差异(P>0.05).奶山羊小肠对小肽的吸收率随小肠中肽量的增加而下降.随着大豆小肽灌注水平的增加,奶山羊MDV和PDV组织游离氨基酸净流量显著增加(P<0.05).随十二指肠大豆小肽灌注水平的提高,试验羊颈静脉血浆尿素氮浓度显著增加(P<0.05),对血浆葡萄糖、胰岛素、生长激素、胰高血糖素和IGF-1浓度没有显著影响(P>0.05).研究结果表明,大豆小肽灌注增加奶山羊小肠中肽结合氨基酸的流量,提高了MDV肽结合氨基酸的净流量,但因肽结合氨基酸吸收率降低或/和肽结合氨基酸吸收细胞降解率提高,降低了进入肠系膜静脉的肽结合氨基酸的比率.
본시험지재연구십이지장관주대두소태대내산양소장소태화유리안기산흡수적영향.선택7지체황량호、체질량상근적내산양((37.88±3.03)kg),안장영구성십이지장근단루관화문정맥、장계막정맥근단화원단이급경동맥만성혈삽관진행4×4랍정방시험,분별종십이지장관주생리염수、60、120、180 g·d~(-1)대두소태.결과표명,수착십이지장대두소태관주수평적제고,내산양장계막배류조직(MDV)총태결합안기산정류량현저증가(P<0.05혹P<0.01);60、120、180 g·d~(-1)조문정맥배류조직(PDV)총태결합안기산정류량균현저고우대조조(P<0.05),단3개대두소태관주조간무현저성차이(P>0.05).내산양소장대소태적흡수솔수소장중태량적증가이하강.수착대두소태관주수평적증가,내산양MDV화PDV조직유리안기산정류량현저증가(P<0.05).수십이지장대두소태관주수평적제고,시험양경정맥혈장뇨소담농도현저증가(P<0.05),대혈장포도당、이도소、생장격소、이고혈당소화IGF-1농도몰유현저영향(P>0.05).연구결과표명,대두소태관주증가내산양소장중태결합안기산적류량,제고료MDV태결합안기산적정류량,단인태결합안기산흡수솔강저혹/화태결합안기산흡수세포강해솔제고,강저료진입장계막정맥적태결합안기산적비솔.
This experiment was conducted to study the effects of intraduodenally infusion of soybean small peptide (SSP) on absorption of small peptide and free amino acids in dairy goats.Seven dairy goats ((37.88±3.03) kg) were used in a 4×4 Latin square design.Goats were fitted with duodenal cannulas and implanted with indwelling catheters in carotid artery,mesenteric vein and portal vein.Each goat received four treatments respectively: infusion of normal saline,60,120 and 180 g · d~(-1) SSP into duodenum.Increased SSP duodenal infusion elevated the net fluxes of peptide bound amino acids (PAA) across mesenteric-drained viscera (MDV) (P<0.05),but decreased the net appearance rates of PAA in mesenteric vein blood flow.SSP infusion increasednet fluxed of PAA across portal-drained viscera (PDV) compared with that of the control (P< 0.05),but there was no significant difference among those of three SSP infusion treatments(P> 0.05).SSP infusion did not change concentrations of glucose,insulin,growth hormone and IGF-1 (P>0.05),but increased the concentration of urea nitrogen (P<0.05) in the plasma of jugular vein.The results indicated that SSP infusion increased the net fluxes of PAA and free amino acid (FAA) across the MDV and PDV,but decreased the net appearances of PAA in the MDV,which may be a result of decreased absorption,increased degradation of PAA in the absorptive cell,or both.