国际外科学杂志
國際外科學雜誌
국제외과학잡지
INTERNATIONAL JOURNAL OF SURGERY
2010年
6期
380-382
,共3页
马建忠%侯朝福%张有成%柳志华
馬建忠%侯朝福%張有成%柳誌華
마건충%후조복%장유성%류지화
专利蓝%脂质体%淋巴造影剂
專利藍%脂質體%淋巴造影劑
전리람%지질체%림파조영제
Patent blue%Liposomos%Lymph-mapping developer
目的 制备淋巴造影剂专利蓝脂质体(PB-LPS).方法 采用被动载药法制备PB-LPS.选用Wistar大鼠、在后足掌皮下注射专利蓝(PB)、PB-LPS、空白脂质体(BL-LPS)或0.9%氯化钠溶液,检测谷丙转氨酶(ALT)、谷草转氨酶(AST)、尿素氮(BUN)、肌酐(Cr),观察淋巴结染色情况.结果 注射PB-LPS、PB后区域淋巴结明显蓝染,两组的总体蓝染率分别是40%和20%(P<0.05),PB-LPS的淋巴标记效果明显优于PB.各组大鼠ALT、AST、BUN、Cr的差异均无统计学意义.结论 被动载药法制备的PB-LPS稳定性好、包封率高.
目的 製備淋巴造影劑專利藍脂質體(PB-LPS).方法 採用被動載藥法製備PB-LPS.選用Wistar大鼠、在後足掌皮下註射專利藍(PB)、PB-LPS、空白脂質體(BL-LPS)或0.9%氯化鈉溶液,檢測穀丙轉氨酶(ALT)、穀草轉氨酶(AST)、尿素氮(BUN)、肌酐(Cr),觀察淋巴結染色情況.結果 註射PB-LPS、PB後區域淋巴結明顯藍染,兩組的總體藍染率分彆是40%和20%(P<0.05),PB-LPS的淋巴標記效果明顯優于PB.各組大鼠ALT、AST、BUN、Cr的差異均無統計學意義.結論 被動載藥法製備的PB-LPS穩定性好、包封率高.
목적 제비림파조영제전리람지질체(PB-LPS).방법 채용피동재약법제비PB-LPS.선용Wistar대서、재후족장피하주사전리람(PB)、PB-LPS、공백지질체(BL-LPS)혹0.9%록화납용액,검측곡병전안매(ALT)、곡초전안매(AST)、뇨소담(BUN)、기항(Cr),관찰림파결염색정황.결과 주사PB-LPS、PB후구역림파결명현람염,량조적총체람염솔분별시40%화20%(P<0.05),PB-LPS적림파표기효과명현우우PB.각조대서ALT、AST、BUN、Cr적차이균무통계학의의.결론 피동재약법제비적PB-LPS은정성호、포봉솔고.
Objective To prepare patent-blue-lipesomes(PB-LPS),a lymph-mapping developer.Methods PB was encapsulated in the liposomes using the passive loading method.The female Wistar rats were injected subcutaneously with the PB-LPS,PB,blank liposomes or isotonic rmtrium chloride at the heel solos,to study the stained situation of the lymph nodes and chemical analysis oftheir ALT,AST,BUN,Cr.Results The lymph nodes were obviously blue staining after injected PB-LPS or PB,the blue smimng ratios were 40%,20%(P<0.05),and the effect of PB-LPS on tracing of lymphy nodes was better than PB.No significant difference was found in ALT,AST,BUN and Cr.Conclusion The PB-LPS can be successfully prefabricated with such advantage as avirulence and high entrapment efficiency by passive loading method.
