CAJ | 학술논문

目的研究静脉应用重组人组织因子途径抑制物-1(reconthinant tissue factor pathway inhibitor-1,rTFPI-1)对兔急性心肌梗死(acute myocardial infarction,AMI)冠状动脉再通后预防血栓形成,挽救存活心肌的作用.方法 40只新西兰大白兔,结扎回旋支中段120 min,再灌注60 min.再灌注即刻随机分为对照组、大、中、小剂量rTFPI-1组,每组10只,活体Evan'S蓝染色测定缺血面积,离体心肌切片TTC染色测定心肌梗死面积.心肌缺血及梗死严重程度分别用缺血面积及梗死面积与左室壁心肌面积的比值表示.各组间基本情况、心肌缺血及梗死严重程度比较用完全随机设计单因素方差分析,均数两两比较采用LSD-t检验.结果大剂量rTFPI一1组心肌梗死严重程度较中、小剂量rTFPI.1组均明显减轻(P<0.05或P<0.01);中剂量rTFPI-1组心肌梗死严重程度较小剂量rTFPI-1组和对照组明显减轻(P<0.01);小剂量rTFPI.1组和对照组比较差异无统计学意义(P>0.05).结论兔闭塞冠状动脉再灌注治疗时予rTFPI-1静点可显著减轻心肌梗死严重程度,挽救存活心肌.
목적 연구정맥응용중조인조직인자도경억제물-1(reconthinant tissue factor pathway inhibitor-1,rTFPI-1)대토급성심기경사(acute myocardial infarction,AMI)관상동맥재통후예방혈전형성,만구존활심기적작용.방법 40지신서란대백토,결찰회선지중단120 min,재관주60 min.재관주즉각수궤분위대조조、대、중、소제량rTFPI-1조,매조10지,활체Evan'S람염색측정결혈면적,리체심기절편TTC염색측정심기경사면적.심기결혈급경사엄중정도분별용결혈면적급경사면적여좌실벽심기면적적비치표시.각조간기본정황、심기결혈급경사엄중정도비교용완전수궤설계단인소방차분석,균수량량비교채용LSD-t검험.결과 대제량rTFPI일1조심기경사엄중정도교중、소제량rTFPI.1조균명현감경(P<0.05혹P<0.01);중제량rTFPI-1조심기경사엄중정도교소제량rTFPI-1조화대조조명현감경(P<0.01);소제량rTFPI.1조화대조조비교차이무통계학의의(P>0.05).결론 토폐새관상동맥재관주치료시여rTFPI-1정점가현저감경심기경사엄중정도,만구존활심기.
Objective To observe the function of recombinant human tissue factor pathway inhibitor1(rTFPI-1)in acute myocardial infarction in rabbit. Method Forty New Zealand White rabbits were subjected to coronary artery occlusion for 120 min and followed by reperfusion for 60 min,then they were ranlow dose rTFPI-1 group(n=10/group).The extent of ischemic area and the extent of myocardial infarction area were measured by Evan's blue stain and TTC stain,respectively.The degrees of infarction severity and ischemic severity were expressed as the ratios of the total left ventrieular wall area.The degrees of infarction severity and ischemic severity in different groups were compared by using one-way ANOVA and then followed by LSD procedure.Results The degree of infarction severity in the larger dose rTFPI-1 group was significantly lessened than that in low dose RTFPI-1 group and control group(P<0.001),and than that in modcrate dose rTFPI-1 group as well(P<0.05).The degree of infarction severity in the moderate dose rTFPI1 group was significantly lessened than that in low dose rTFPI-1 group and control group(P<0.001).There was no significant difference in degree of infarction severity between low dose rTFPI-1 group and control group(P>0.05).Conclusions Human rTFPI-1 might decrease myocardial infarction severity and save the survival myocardial tissue.