CAJ | 학술논문

目的探讨结直肠癌组织中GRP78、GRP94的表达与患者临床病理参数及预后的关系.方法应用免疫组织化学技术分析124例结直肠癌组织中GRP78、GRP94表达水平.免疫印迹法检测其蛋白表达水平验证免疫组化结果.将免疫组化检测结果与患者的临床病理特征及生存时间进行单因素及多因素回归统计学分析.结果 GRP78、GRP94阳性表达均定位于细胞质及少部分包膜,而临近的正常肠组织中少见阳性表达.免疫印迹法检测蛋白表达水平与免疫组化结果一致.单因素分析GRP78和GRP94蛋白表达在分化程度、分期、淋巴结转移及远处转移状态间差异有统计学意义.生存分析显示GRP78、GRP94蛋白高表达患者生存时间较低表达患者明显缩短,差异有统计学意义(P =0.025和P=0.010).多因素分析显示GRP94表达是结直肠癌的独立预后指标.结论结直肠癌组织中GRP78、GRP94过表达可能与结直肠癌的发生、生长、侵袭、转移及预后相关,可认为是肿瘤进展和预后的客观指标.
목적 탐토결직장암조직중GRP78、GRP94적표체여환자림상병리삼수급예후적관계.방법 응용면역조직화학기술분석124례결직장암조직중GRP78、GRP94표체수평.면역인적법검측기단백표체수평험증면역조화결과.장면역조화검측결과여환자적림상병리특정급생존시간진행단인소급다인소회귀통계학분석.결과 GRP78、GRP94양성표체균정위우세포질급소부분포막,이림근적정상장조직중소견양성표체.면역인적법검측단백표체수평여면역조화결과일치.단인소분석GRP78화GRP94단백표체재분화정도、분기、림파결전이급원처전이상태간차이유통계학의의.생존분석현시GRP78、GRP94단백고표체환자생존시간교저표체환자명현축단,차이유통계학의의(P =0.025화P=0.010).다인소분석현시GRP94표체시결직장암적독립예후지표.결론 결직장암조직중GRP78、GRP94과표체가능여결직장암적발생、생장、침습、전이급예후상관,가인위시종류진전화예후적객관지표.
Objective To study roles of GRP78 and GRP94 in tumorigenesis and progression of colorectal carcinomas (CRC).Methods Colorectal carcinoma tissues of 124 cases were studied for GRP78 and GRP94 proteins by immunohistochemistry and Western blotting.The correlations among the expression of proteins and clinicopathologic parameters and overall survival of CRC were analyzed using univariate and Cox regression analyses.Results The overexpression of GRP78 and GRP94 was detected in the cytoplasms or plasma membrane of the tumor cells.They were positively correlated with tumor differentiation,staging,lymph node metastasis and remote metastasis.Survival analysis showed that overexpression of both proteins was associated with poor outcome of CRC ( P =0.025,P =0.010,respectively).Cox regression analysis demonstrated that the expression of GRP94 was an independent poor prognostic factor for CRC.Conclusions Up-regulated expression of GRP78 and GRP94 was possibly involved in the pathogenesis,growth,invasion and metastasis of colorectal carcinomas.GRP78 and GRP94could be seen as a marker for the aggressive behavior and poor prognosis in colorectal carcinomas.