中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2011年
9期
793-795
,共3页
王前%殷盛明%李盛龙%徐红%于德钦%王伟阁%熊祖明%吴杰%王冬梅%孙艺平%李崧%唐一源
王前%慇盛明%李盛龍%徐紅%于德欽%王偉閣%熊祖明%吳傑%王鼕梅%孫藝平%李崧%唐一源
왕전%은성명%리성룡%서홍%우덕흠%왕위각%웅조명%오걸%왕동매%손예평%리숭%당일원
社会隔离%空间认知功能%非空间认知功能
社會隔離%空間認知功能%非空間認知功能
사회격리%공간인지공능%비공간인지공능
Social isolation%Spatial cognitive ability%Nonspatial cognitive ability
目的 探讨社会隔离对小鼠空间和非空间认知功能的影响.方法 选取健康生后21天昆明小鼠,按照随机区组化分为对照组、隔离2周组、隔离4周组、隔离8周组和隔离2周后群居组,每组10只.隔离2周组、隔离4周组和隔离8周组分别于生后21天起,单笼饲养2周、4周和8周,隔离2周后群居组于隔离2周后恢复群居直至成年,对应相同月龄的正常饲养小鼠作为对照组.所有实验动物于干预后进行物体识别实验和物体定位实验测试,来评估其空间和非空间认知功能改变.结果 在物体识别实验中,与相应对照组相比,隔离2周、隔离4周和隔离8周小鼠的辨别指数[分别为(-0.03±0.003),(-0.11±0.02),(-0.21±0.02)],明显低于对照组[(分别为(0.29±0.03),(0.13±0.07),(0.09±0.03),p＜0.05]；在物体定位实验中,与相应对照组相比,隔离2周、隔离4周和隔离8周小鼠的辨别指数[分别为(-0.15±0.02),(-0.30±0.02),(-0.32±0.02)],明显低于对照组[(分别为(0.33±0.02),(0.41 ±0.03),(0.27±0.04),P＜0.05].隔离2周后群居组小鼠的辨别指数都无统计学意义.结论 小鼠隔离2周、4周和8周后,空间和非空间认知功能降低,而隔离2周后恢复的群居生活能够改善小鼠的空间与非空间认知能力.
目的 探討社會隔離對小鼠空間和非空間認知功能的影響.方法 選取健康生後21天昆明小鼠,按照隨機區組化分為對照組、隔離2週組、隔離4週組、隔離8週組和隔離2週後群居組,每組10隻.隔離2週組、隔離4週組和隔離8週組分彆于生後21天起,單籠飼養2週、4週和8週,隔離2週後群居組于隔離2週後恢複群居直至成年,對應相同月齡的正常飼養小鼠作為對照組.所有實驗動物于榦預後進行物體識彆實驗和物體定位實驗測試,來評估其空間和非空間認知功能改變.結果 在物體識彆實驗中,與相應對照組相比,隔離2週、隔離4週和隔離8週小鼠的辨彆指數[分彆為(-0.03±0.003),(-0.11±0.02),(-0.21±0.02)],明顯低于對照組[(分彆為(0.29±0.03),(0.13±0.07),(0.09±0.03),p＜0.05]；在物體定位實驗中,與相應對照組相比,隔離2週、隔離4週和隔離8週小鼠的辨彆指數[分彆為(-0.15±0.02),(-0.30±0.02),(-0.32±0.02)],明顯低于對照組[(分彆為(0.33±0.02),(0.41 ±0.03),(0.27±0.04),P＜0.05].隔離2週後群居組小鼠的辨彆指數都無統計學意義.結論 小鼠隔離2週、4週和8週後,空間和非空間認知功能降低,而隔離2週後恢複的群居生活能夠改善小鼠的空間與非空間認知能力.
목적 탐토사회격리대소서공간화비공간인지공능적영향.방법 선취건강생후21천곤명소서,안조수궤구조화분위대조조、격리2주조、격리4주조、격리8주조화격리2주후군거조,매조10지.격리2주조、격리4주조화격리8주조분별우생후21천기,단롱사양2주、4주화8주,격리2주후군거조우격리2주후회복군거직지성년,대응상동월령적정상사양소서작위대조조.소유실험동물우간예후진행물체식별실험화물체정위실험측시,래평고기공간화비공간인지공능개변.결과 재물체식별실험중,여상응대조조상비,격리2주、격리4주화격리8주소서적변별지수[분별위(-0.03±0.003),(-0.11±0.02),(-0.21±0.02)],명현저우대조조[(분별위(0.29±0.03),(0.13±0.07),(0.09±0.03),p＜0.05]；재물체정위실험중,여상응대조조상비,격리2주、격리4주화격리8주소서적변별지수[분별위(-0.15±0.02),(-0.30±0.02),(-0.32±0.02)],명현저우대조조[(분별위(0.33±0.02),(0.41 ±0.03),(0.27±0.04),P＜0.05].격리2주후군거조소서적변별지수도무통계학의의.결론 소서격리2주、4주화8주후,공간화비공간인지공능강저,이격리2주후회복적군거생활능구개선소서적공간여비공간인지능력.
Objective To study the effects of social isolation (SI)on the spatial and nonspatial cognitive ability in mice.Methods The postnatal 21 day kunming mice were divided into control group,SI 2 weeks group,SI 4 weeks group,SI 8 weeks group and SI 2 weeks gregarious group according to randomized block design,with ten animals each.SI 2 weeks group,SI 4 weeks group and SI 8 weeks group were isolated for 2,4 and 8 weeks respectively,SI 2 weeks gregarious group would be housed under normal grouped housing condition after 2 weeks isolation until adult,the relative control groups were the same age as the relative SI and SI gregarious group.All animals were measured the spatial and nonspatial cognitive ability by carrying the object recognition test(ORT) and object location test (OLT) after the treatment.Results In the ORT,compared to the relative control group,the discrimination index in the SI 2 weeks group,SI 4 weeks group and SI 8 weeks group ( ( - 0.03 ± 0.003 ),( - 0.11 ±0.02) and( - 0.21 ± 0.02 ) respectively) were strikingly lower than the relative control group ( ( 0.29 ± 0.03 ),(0.13±0.07) and (0.09 ±0.03) respectively) (P＜0.05).In the OLT,compared to the relative control group,the discrimination index in the SI 2 weeks group,SI 4 weeks group and SI 8 weeks group( ( -0.15 ±0.02),( -0.30± 0.02),( - 0.32 ± 0.02 ) respectively ) were strikingly lower than the relative control group ( (0.33 ± 0.02 ),(0.41 ± 0.03 ),(0.27 ± 0.04)respectively)(P＜ 0.05 ),while the SI 2 weeks gregarious group with the resocialization to the normal housing condition showed no change.Conclusions 2 weeks,4 weeks and 8 weeks isolation on mice lead to the spatial and nonspatial cognition deficits,while the resocialization to the normal housing condition could recover the damage.
