CAJ | 학술논문

实时荧光定量PCR方法检测大鼠棕色脂肪和骨骼肌中PPARγ辅激活因子1α(PGC-1α)mRNA的表达.肥胖大鼠棕色脂肪和骨骼肌中PGC-1α mRNA表达水平低于普通大鼠(均P<0.01);应用高剂量糖皮质激素后普通大鼠和肥胖大鼠棕色脂肪和骨骼肌中PGC-1α mRNA表达水平降低.
실시형광정량PCR방법검측대서종색지방화골격기중PPARγ보격활인자1α(PGC-1α)mRNA적표체.비반대서종색지방화골격기중PGC-1α mRNA표체수평저우보통대서(균P<0.01);응용고제량당피질격소후보통대서화비반대서종색지방화골격기중PGC-1α mRNA표체수평강저.
Real-time fluorescent quantitative PCR was used to examine PGC-1α mRNA expression in brown adipose tissue and skeletal muscle of rats. The results showed that the expression levels of PGC-1α mRNA in brown adipose tissue and skeletal muscle of obese rats were lower than those of the normal ones (all P<0.01). After high dose glucocorticoid treatment, the levels of PGC-1α mRNA expression in brown adipose tissue and skeletal muscle, both in normal and obese rats, were decreased significantly.