CAJ | 학술논문

研究Pax6基因纯合突变胎鼠上前牙区额外牙的发生情况,为研究Pax6在上前牙发生、发育中的作用及额外牙的发生机制提供依据.方法收集E18.5 DEBA Pax6基因纯合突变胎鼠20只,制作胎鼠头部组织冠状面石蜡连续切片,HE染色,观察上前牙区额外牙的发生情况;E18.5 C57BL/6NCrlBR Pax6基因野生型胎鼠18只作为对照.结果 20只Pax6基因纯合突变胎鼠中,4只上前牙区显示有额外牙牙胚;18只Pax6基因野生型胎鼠上前牙区均无额外牙牙胚.Pax6基因纯合突变胎鼠和Pax6基因野生型胎鼠的下前牙区和上下颌第一、第二磨牙区牙胚的数目相同,形态结构未见明显差别.结论本实验结果提示Pax6基因在胎鼠上前牙发生过程中有非常重要的作用.
연구Pax6기인순합돌변태서상전아구액외아적발생정황,위연구Pax6재상전아발생、발육중적작용급액외아적발생궤제제공의거.방법수집E18.5 DEBA Pax6기인순합돌변태서20지,제작태서두부조직관상면석사련속절편,HE염색,관찰상전아구액외아적발생정황;E18.5 C57BL/6NCrlBR Pax6기인야생형태서18지작위대조.결과 20지Pax6기인순합돌변태서중,4지상전아구현시유액외아아배;18지Pax6기인야생형태서상전아구균무액외아아배.Pax6기인순합돌변태서화Pax6기인야생형태서적하전아구화상하합제일、제이마아구아배적수목상동,형태결구미견명현차별.결론본실험결과제시Pax6기인재태서상전아발생과정중유비상중요적작용.
Objective To investigate the occurrence of supernumerary upper incisor teeth in Pax6-/- mouse fetuses and to provide a model to explore the role of Pax6 in the upper incisor development and the mechanism of supernumerary teeth involving Pax6. Methods Twenty Pax6-/- mouse fetuses of strain DEBA were isolated on E18.5 (embryonic day). The fetuses were sectioned serially in coronal plane and stained with haematoxylin and ernsin, then the presence of supernumerary teeth in the upper anterior area was examined histologically, and also the number, morphology and structure of lower incisor germs and the first and second molar germs in the maxilla and mandible were observed histologically. Eighteen E18.5 mouse fetuses of strain DEBA with Pax6 +/+ genotype were used as control. Results Of the 20 Pax6-/- fetuses examined, four possessed a single supernumerary tooth in the upper inciors' region. No supernumerary upper incisor teeth were observed in any of the 18 Pax6 +/+ fetuses examined. In the regions of lower incisors and the first and second molars of the maxilla and mandible, no significant difference was observed between Pax6-/- and Pax6 +/+ fetuses regarding the number, morphology and structure of tooth germs. Conclusions The results suggest that Pax6 played an important role in the development of upper incisor teeth in mice.