CAJ | 학술논문

目的观察七叶皂苷钠对人胆管癌细胞株Hccc9810增殖和凋亡的影响.方法运用细胞染色、噻唑蓝(MTT)比色法、流式细胞术和实时荧光定量聚合酶链反应(PCR)等技术观察不同浓度(0、10、20、40 μmol/L)七叶皂苷钠对胆管癌细胞增殖、细胞周期和凋亡的影响.结果七叶皂苷钠呈时间剂量依赖性抑制胆管癌细胞Hccc9810的增殖,其24、48、72 h的50％抑制浓度(IC50)分别为:(45.34±2.12)、(33.00±1.92)、(25.00±1.65) μmol/L;G1期比例从(49.49±1.22)％上升到(70.20±1.52)％；胆管癌细胞形态发生典型凋亡特征性改变,凋亡率随浓度的升高从20.54％上升到62.56％,差异均有统计学意义(P＜0.05).结论七叶皂苷钠通过阻滞细胞周期和诱导细胞凋亡而抑制胆管癌细胞Hccc9810增殖.
목적 관찰칠협조감납대인담관암세포주Hccc9810증식화조망적영향.방법 운용세포염색、새서람(MTT)비색법、류식세포술화실시형광정량취합매련반응(PCR)등기술관찰불동농도(0、10、20、40 μmol/L)칠협조감납대담관암세포증식、세포주기화조망적영향.결과 칠협조감납정시간제량의뢰성억제담관암세포Hccc9810적증식,기24、48、72 h적50％억제농도(IC50)분별위:(45.34±2.12)、(33.00±1.92)、(25.00±1.65) μmol/L;G1기비례종(49.49±1.22)％상승도(70.20±1.52)％；담관암세포형태발생전형조망특정성개변,조망솔수농도적승고종20.54％상승도62.56％,차이균유통계학의의(P＜0.05).결론 칠협조감납통과조체세포주기화유도세포조망이억제담관암세포Hccc9810증식.
Objective To investigate the effects of sodium aescinate on the proliferation and apoptosis of cholangiocarcinoma Hccc9810 cell line.Methods The effects of sodium aescinate (0,10,20,40μmol/L) on cell growth,cycle and apoptosis were investigated by methyl thiazol tetrazolium (MTT) assay,flow cytometry,and fluorescence microscopy.Results Sodium aescinate inhibited cholangiocarcinoma cell growth in a dose- and time-dependent manner,and its half-inhibitory concentration (IC50) for 24 h,48 h,and 72 h was (45.34 ±2.12),(33.00± 1.92) and (25.00 ± 1.65) μmol/L respectively.The proportion of cells in G1 phase was increased from (49.49 ± 1.22)％ to (70.20 ± 1.52)％.The apoptosis rote was increased from 20.54％ to 62.56％ (P ＜0.05).Conclusion Sodium aescinate exhibited its anticarcinoma effect probably through regulating cell cycle and inducing cell apoptosis.