CAJ | 학술논문

目的通过建立新西兰白兔肺外科单侧肺缺血-再灌注损伤动物模型,观察其缺血-再灌注损伤特点.方法将96只健康新西兰兔随机分成3组:Ⅰ组为对照组(n=36);Ⅱ组为单纯阻断肺动脉组(n=30);Ⅲ组为阻断肺循环组(n=30).术中监测动物血流动力学指标;分别于开胸时(Ⅰ组)、缺血1小时和再灌注1、2、4、6 h(Ⅰ、Ⅱ、Ⅲ组)取肺组织检测MDA;取肺组织作病理学观察,并测定手术侧肺湿/干比.结果各组动物术中血流动力学指标平稳;在缺血1 h和再灌注1、2、4、6 h,Ⅲ组与Ⅱ组比较,肺湿/干比及肺组织中MDA含量的差异均无统计学意义(P＞0.05),Ⅰ组与Ⅱ、Ⅲ组比较差异有统计学意义(P＜0.05).在相同的时间点,Ⅱ、Ⅲ组呈现相似的肺损伤病理改变,且较为明显的肺损伤均出现在再灌注4h,再灌注6h肺损伤均呈恢复趋势.结论肺外科中肺动脉阻断和肺循环阻断所致肺缺血一再灌注损伤特点相似,肺外科中肺血管阻断1h是安全的.
목적 통과건립신서란백토폐외과단측폐결혈-재관주손상동물모형,관찰기결혈-재관주손상특점.방법 장96지건강신서란토수궤분성3조:Ⅰ조위대조조(n=36);Ⅱ조위단순조단폐동맥조(n=30);Ⅲ조위조단폐순배조(n=30).술중감측동물혈류동역학지표;분별우개흉시(Ⅰ조)、결혈1소시화재관주1、2、4、6 h(Ⅰ、Ⅱ、Ⅲ조)취폐조직검측MDA;취폐조직작병이학관찰,병측정수술측폐습/간비.결과 각조동물술중혈류동역학지표평은;재결혈1 h화재관주1、2、4、6 h,Ⅲ조여Ⅱ조비교,폐습/간비급폐조직중MDA함량적차이균무통계학의의(P＞0.05),Ⅰ조여Ⅱ、Ⅲ조비교차이유통계학의의(P＜0.05).재상동적시간점,Ⅱ、Ⅲ조정현상사적폐손상병리개변,차교위명현적폐손상균출현재재관주4h,재관주6h폐손상균정회복추세.결론 폐외과중폐동맥조단화폐순배조단소치폐결혈일재관주손상특점상사,폐외과중폐혈관조단1h시안전적.
Objective To establish pulmonary vessel blocking model in rabbits and to investigate lung ischemia-reperfusion injury in pulmonary surgery.Methods Ninety-six New Zealand rabbits were randomly divided into 3 groups:group Ⅰ,control group;group Ⅱ,blocking pulmonary artery;group Ⅲ,blocking pulmonary artedes and veins.The content of MDA and the wet/dry ratio of the lung tissue were recorded at the time of opening chest(group Ⅰ),1h ischemia and 1,2,4,6 h reperfusion.Pathological changes of the lung were also observed at each time point.Results Homodynamic parameters were stable in all 3 groups.There was no significant difference in MDA and wet/dry ratio of the lung tissue between group Ⅱ and group Ⅲ(P＞0.05),but there was significant difference between group Ⅰ and other two groups(P＜0.05).Pathological study revealed that similar ischemia-reperfusion injury changes happened in both group Ⅱ and group Ⅲ,and the obvious injury happened at the time of 4 h repeffusion.At the time of 6 h reperfusion pathological changes in both group Ⅱ and group Ⅲ began to recover.Conclusion Lung ischemia-reperfusion injury caused by blocking pulmonary artery is similar to that caused by blocking pulmonary arteries and veins.It is safe to previously block pulmonary vessel8 within 1h during pulmonary surgery.