中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2012年
4期
259-262
,共4页
张国英%浦声波%于学英%杨建%石建凤%许爱玲%郭振华%乔明刚%张杰%荆永正
張國英%浦聲波%于學英%楊建%石建鳳%許愛玲%郭振華%喬明剛%張傑%荊永正
장국영%포성파%우학영%양건%석건봉%허애령%곽진화%교명강%장걸%형영정
肝炎病毒属%基因型%序列分析%种系发生
肝炎病毒屬%基因型%序列分析%種繫髮生
간염병독속%기인형%서렬분석%충계발생
Hepatitis viruses%Genotype%Sequence analysis%Phylogeny
目的 了解山东烟台地区丙型肝炎病毒的基因分型,结合受试者的肝功能指标观察基因型别与肝损情况是否相关.方法 采用特异性PCR引物对HCV RNA5'UTR区和(或)NS5B区进行扩增,PCR产物进行序列分析,通过与GenBank中参考序列的比对,联合遗传进化树对标本予以分型.结果 9例无偿献血员中检出1b和3a两种基因亚型,分别为8例和1例.33例丙肝患者中,检出1b、2a和6a三种基因亚型,分别为22(66.7％)、10(30.3％)和1(3.03％)例.1b亚型是烟台地区HCV携带者的优势流行基因亚型,在不同人群中分布差异无统计学意义(x2=0.796,P=0.373)；不同基因分型的受试者其肝损指标的差异有统计学意义(P＜0.05),2a型携带者的ALT、AST均值明显高于1b型.结论 山东烟台地区HCV基因型呈现多样性,以1b为主,并首次检出3a和6a亚型.HCV基因型与肝损指标具有相关性,2a型HCV感染可能在肝细胞的病变过程中起着重要作用.
目的 瞭解山東煙檯地區丙型肝炎病毒的基因分型,結閤受試者的肝功能指標觀察基因型彆與肝損情況是否相關.方法 採用特異性PCR引物對HCV RNA5'UTR區和(或)NS5B區進行擴增,PCR產物進行序列分析,通過與GenBank中參攷序列的比對,聯閤遺傳進化樹對標本予以分型.結果 9例無償獻血員中檢齣1b和3a兩種基因亞型,分彆為8例和1例.33例丙肝患者中,檢齣1b、2a和6a三種基因亞型,分彆為22(66.7％)、10(30.3％)和1(3.03％)例.1b亞型是煙檯地區HCV攜帶者的優勢流行基因亞型,在不同人群中分佈差異無統計學意義(x2=0.796,P=0.373)；不同基因分型的受試者其肝損指標的差異有統計學意義(P＜0.05),2a型攜帶者的ALT、AST均值明顯高于1b型.結論 山東煙檯地區HCV基因型呈現多樣性,以1b為主,併首次檢齣3a和6a亞型.HCV基因型與肝損指標具有相關性,2a型HCV感染可能在肝細胞的病變過程中起著重要作用.
목적 료해산동연태지구병형간염병독적기인분형,결합수시자적간공능지표관찰기인형별여간손정황시부상관.방법 채용특이성PCR인물대HCV RNA5'UTR구화(혹)NS5B구진행확증,PCR산물진행서렬분석,통과여GenBank중삼고서렬적비대,연합유전진화수대표본여이분형.결과 9례무상헌혈원중검출1b화3a량충기인아형,분별위8례화1례.33례병간환자중,검출1b、2a화6a삼충기인아형,분별위22(66.7％)、10(30.3％)화1(3.03％)례.1b아형시연태지구HCV휴대자적우세류행기인아형,재불동인군중분포차이무통계학의의(x2=0.796,P=0.373)；불동기인분형적수시자기간손지표적차이유통계학의의(P＜0.05),2a형휴대자적ALT、AST균치명현고우1b형.결론 산동연태지구HCV기인형정현다양성,이1b위주,병수차검출3a화6a아형.HCV기인형여간손지표구유상관성,2a형HCV감염가능재간세포적병변과정중기착중요작용.
Objective To understand the hepatitis C virus (HCV) genotype distribution in Yantai district of Shandong province,and to explore whether the HCV genotypes was relevant to the injure of liver through the index of liver function.Methods Using specific PCR primers to amplify the HCV RNA 5' UTR/NS5B,then PCR products were sequenced by genetic analyzer.The genotypes were identified by alignment to the GenBank reference sequences and construction the phylogenetic tree of 5 ' UTR.Results Among 9 unpaid blood donors we detected two kinds of genotypes of 1b and 3a,respectively,8 cases (88.9％) and 1 case (11.1％).Among 33 cases of hepatitis C patients we detected the lb,2a and 6a the three kinds of genotypes,respectively,22 (66.7％),10 (30.3％ ) and 1 (3.03％) cases.Subtype 1b is the advantage of popular genotype in HCV carriers from Yantai district,and the distribution of 1b was no significant difference in the different population (x2 =0.796,P =0.373 ) ; The difference of indicator of liver damage in the different genotypes of subjects were significant ( P ＜ 0.05 ),the mean of ALT,AST of 2asubtype carriers was significantly higher than the 1 b-subtype population.Conclusions The genetic diversity of HCV in Shandong Yantai district was presented.The main genotypes were 1b-subtype,and 3a and 6asubtype was detected firstly.The genotype of HCV were relevant to the liver damage indicators,2a-subtype hepatitis C virus infection in the liver cells may play an important role in the disease process.
