中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2010年
8期
760-764
,共5页
强直性脊柱炎%Fc受体样基因3%单核苷酸多态性%单倍型
彊直性脊柱炎%Fc受體樣基因3%單覈苷痠多態性%單倍型
강직성척주염%Fc수체양기인3%단핵감산다태성%단배형
Ankylosing spondylitis%Fc receptor like 3%Single nucleotide polymorphism%Haplotype
目的 探讨Fc受体样基因3(FcRL3)部分单核苷酸多态性(SNP)在中国汉族强直性脊柱炎(AS)发病中的作用.方法 选取AS患者160例,健康对照组170例.用Mass-ArrayTM SNP检测仪对FcRL3基因上的SNP位点:FcRL3-1(rs0158440)、FcRL3-2(rs2225828)、FcRL3-3(rs7528684)、FcRL3-4(rs11284799)、FcRL3-5(rs945635)、FcRL3-6(rs3761959)、FcRL3-7(rs2210913)、FcRL3-8(rs2282284)、FcRL3-9(rs2282283)SNP进行检测.单倍型构建采用PHASEv2.1软件完成.结果 在AS组中FcRL3-1-1651C、FcRL3-3-169C、FcRL3-6 Intron3A、FcRL3-7 Intron3A等位基因频率病例组高于对照组;FcRL3-2-641C、FcRI3-4-110A、FcRL3-9 Intron15A等位基因频率病例组低于对照组;FcRL3-6、FcRL3-5和FcRL3-8SNP等位基因两组比较差异无统计学意义.AS组中单倍型FcRL3-1→9 CTCGCAAAA、CCCGGGGAA、CCCGCAAAC、CTCGCAAAC频率明显高于对照组;FcRL3-1→9 CCTGGGGAA和TCCACAAGA频率明显低于对照组.结论 FcRL3基因与中国汉族AS的易感性相关.
目的 探討Fc受體樣基因3(FcRL3)部分單覈苷痠多態性(SNP)在中國漢族彊直性脊柱炎(AS)髮病中的作用.方法 選取AS患者160例,健康對照組170例.用Mass-ArrayTM SNP檢測儀對FcRL3基因上的SNP位點:FcRL3-1(rs0158440)、FcRL3-2(rs2225828)、FcRL3-3(rs7528684)、FcRL3-4(rs11284799)、FcRL3-5(rs945635)、FcRL3-6(rs3761959)、FcRL3-7(rs2210913)、FcRL3-8(rs2282284)、FcRL3-9(rs2282283)SNP進行檢測.單倍型構建採用PHASEv2.1軟件完成.結果 在AS組中FcRL3-1-1651C、FcRL3-3-169C、FcRL3-6 Intron3A、FcRL3-7 Intron3A等位基因頻率病例組高于對照組;FcRL3-2-641C、FcRI3-4-110A、FcRL3-9 Intron15A等位基因頻率病例組低于對照組;FcRL3-6、FcRL3-5和FcRL3-8SNP等位基因兩組比較差異無統計學意義.AS組中單倍型FcRL3-1→9 CTCGCAAAA、CCCGGGGAA、CCCGCAAAC、CTCGCAAAC頻率明顯高于對照組;FcRL3-1→9 CCTGGGGAA和TCCACAAGA頻率明顯低于對照組.結論 FcRL3基因與中國漢族AS的易感性相關.
목적 탐토Fc수체양기인3(FcRL3)부분단핵감산다태성(SNP)재중국한족강직성척주염(AS)발병중적작용.방법 선취AS환자160례,건강대조조170례.용Mass-ArrayTM SNP검측의대FcRL3기인상적SNP위점:FcRL3-1(rs0158440)、FcRL3-2(rs2225828)、FcRL3-3(rs7528684)、FcRL3-4(rs11284799)、FcRL3-5(rs945635)、FcRL3-6(rs3761959)、FcRL3-7(rs2210913)、FcRL3-8(rs2282284)、FcRL3-9(rs2282283)SNP진행검측.단배형구건채용PHASEv2.1연건완성.결과 재AS조중FcRL3-1-1651C、FcRL3-3-169C、FcRL3-6 Intron3A、FcRL3-7 Intron3A등위기인빈솔병례조고우대조조;FcRL3-2-641C、FcRI3-4-110A、FcRL3-9 Intron15A등위기인빈솔병례조저우대조조;FcRL3-6、FcRL3-5화FcRL3-8SNP등위기인량조비교차이무통계학의의.AS조중단배형FcRL3-1→9 CTCGCAAAA、CCCGGGGAA、CCCGCAAAC、CTCGCAAAC빈솔명현고우대조조;FcRL3-1→9 CCTGGGGAA화TCCACAAGA빈솔명현저우대조조.결론 FcRL3기인여중국한족AS적역감성상관.
Objective To investigate the association between a few single nucleotide polymorphisms(SNPs) in Fc receptor like 3 and ankylosing spondylitis(AS) in Hah nationality of China patients.Methods One hundred and sixty AS patients along with one hundred and seventy healthy controls were included in the study. SNPs of FcRL3-1 (rs0158440), FcRL3-2 ( rs2225828 ), FcRL3-3 ( rs7528684 ),FcRL3-4(rs11284799), FcRL3-5(rs945635), FcRL3-6(rs3761959), FcRL3-7 (rs2210913), FcRL3-8 (ra2282284) and FcRL3-9 (rs2282283) in FcRL3 gene were genotyped by MALDI-TOF technology. Haplotypes were estimated using PHASE v2.1 software. Results The frequency of FcRL3-1-1651C, FcRL3-3-169C, FcRL3-6 Intron3A and FcRL3-7 Intron3A alleles in AS patients were significantly increased and the frequency of FcRL3-2-641C, FcR-L3-4-110A and FcRL3-9 Intron15A were significantly increased compared with healthy controls. The frequency of FcRL3-1→9 CTCGCAAAA, CCCGGGGAA, CCCGCAAAC and CTCGCAAAC haplotypes were significantly increased and the frequency of FcRL3-1→9CCTGGGAA and TCCACAAGA haplotypes were significantly decreased compared with healthy controls. Conclusion These results suggest that FcRL3 may be associated with AS in Han nationality of China.
