植物学报
植物學報
식물학보
ACTA BOTANICA SINICA
2001年
5期
480-485
,共6页
宋平%夏凯%吴传万%包冬萍%陈丽莉%周燮%曹显祖
宋平%夏凱%吳傳萬%包鼕萍%陳麗莉%週燮%曹顯祖
송평%하개%오전만%포동평%진려리%주섭%조현조
水稻雄性不育系%保持系%开颖%茉莉酸甲酯%水杨酸%24-表油菜素内酯
水稻雄性不育繫%保持繫%開穎%茉莉痠甲酯%水楊痠%24-錶油菜素內酯
수도웅성불육계%보지계%개영%말리산갑지%수양산%24-표유채소내지
以水稻(Oryza sativa subsp.indica)雄性不育系和可育系为材料，在室内用溶液浸已开过一天花的水稻离体穗2min，发现4mmol/L茉莉酸甲酯（MeJA）能显著诱导水稻开颖，且MeJA诱导水稻雄性不育材料开颖的效应明显强于可育材料（如保持系和常规品种），MeJA使雄性不育系珍汕97A、温敏雄性核不育系培矮64S的当天最后开颖率达到40%以上，保持系珍汕97B、常规品种“培矮64”的当天最后开颖率仅有(21±1.5)%、(25±2.1)%，对照穗在当天始终不开颖或开颖极少（<5%）；不育系开颖对MeJA响应的滞后期比可育系短10min左右。在田间，2mmol/LMeJA溶液浸或喷雾水稻连体穗，也同样能明显提高培矮64S的开颖率；辅以人工授粉，MeJA能大幅度提高珍汕97A、龙特浦A的结实率（提高24%以上）。1mmol/L水杨酸（SA）能抑制MeJA诱导水稻开颖，而MeJA的再次处理能解除SA对MeJA诱导雄性不育系开颖的抑制效应，SA抑制MeJA诱导保持系开颖的效应不能被再次使用MeJA所解除。24-表油菜素内酯（24-epiBL）预先处理能显著增强MeJA诱导不育系开颖，而对于保持系，此效应不明显。
以水稻(Oryza sativa subsp.indica)雄性不育繫和可育繫為材料，在室內用溶液浸已開過一天花的水稻離體穗2min，髮現4mmol/L茉莉痠甲酯（MeJA）能顯著誘導水稻開穎，且MeJA誘導水稻雄性不育材料開穎的效應明顯彊于可育材料（如保持繫和常規品種），MeJA使雄性不育繫珍汕97A、溫敏雄性覈不育繫培矮64S的噹天最後開穎率達到40%以上，保持繫珍汕97B、常規品種“培矮64”的噹天最後開穎率僅有(21±1.5)%、(25±2.1)%，對照穗在噹天始終不開穎或開穎極少（<5%）；不育繫開穎對MeJA響應的滯後期比可育繫短10min左右。在田間，2mmol/LMeJA溶液浸或噴霧水稻連體穗，也同樣能明顯提高培矮64S的開穎率；輔以人工授粉，MeJA能大幅度提高珍汕97A、龍特浦A的結實率（提高24%以上）。1mmol/L水楊痠（SA）能抑製MeJA誘導水稻開穎，而MeJA的再次處理能解除SA對MeJA誘導雄性不育繫開穎的抑製效應，SA抑製MeJA誘導保持繫開穎的效應不能被再次使用MeJA所解除。24-錶油菜素內酯（24-epiBL）預先處理能顯著增彊MeJA誘導不育繫開穎，而對于保持繫，此效應不明顯。
이수도(Oryza sativa subsp.indica)웅성불육계화가육계위재료，재실내용용액침이개과일천화적수도리체수2min，발현4mmol/L말리산갑지（MeJA）능현저유도수도개영，차MeJA유도수도웅성불육재료개영적효응명현강우가육재료（여보지계화상규품충），MeJA사웅성불육계진산97A、온민웅성핵불육계배왜64S적당천최후개영솔체도40%이상，보지계진산97B、상규품충“배왜64”적당천최후개영솔부유(21±1.5)%、(25±2.1)%，대조수재당천시종불개영혹개영겁소（<5%）；불육계개영대MeJA향응적체후기비가육계단10min좌우。재전간，2mmol/LMeJA용액침혹분무수도련체수，야동양능명현제고배왜64S적개영솔；보이인공수분，MeJA능대폭도제고진산97A、룡특포A적결실솔（제고24%이상）。1mmol/L수양산（SA）능억제MeJA유도수도개영，이MeJA적재차처리능해제SA대MeJA유도웅성불육계개영적억제효응，SA억제MeJA유도보지계개영적효응불능피재차사용MeJA소해제。24-표유채소내지（24-epiBL）예선처리능현저증강MeJA유도불육계개영，이대우보지계，차효응불명현。
Effects of methyl jasmonate (MeJA) on floret opening wereinvestigated in male-s terile and male-fertile rice (Oryza sativa subsp. indica ). Excised or intact panicles with several top florets flowered one day before the experiments were soaked in MeJA, salicylic acid (SA), 24-epibrassinolide (24- epiBL) or control solution for 2 min. MeJA at the concentration of 4 mmol/L significantly promoted rice floret opening; the response of floret opening of male-s terile rice to MeJA was more sensitive than that of male-fertile rice (such as maintenance line and conventional variety). The percentage of finally opened-floret per panicle of Zhenshan 97A (cytoplasmic male sterile line) or Pei'ai 64S (thermo-sensitive genic male sterile line) treated with MeJA was over 40% in the day of experiment, that of Zhenshan 97B (maintenance) and Pei'ai 64 (conventio nal variety) was (21±1.5)% and (25±2.1)%, respectively; floret opening of intact Pei'ai 64S panicles in the field could also be induced by 2-mmol/L MeJA. The percentage of opened floret in all control panicles was 0% or less than 5% in the day of experiment. The percentage of fertilized-grain of Zhenshan 97A and Long tepu A could be increased over 24% by spraying panicles with 2-mmol/L MeJA solut ion （circa 6.0 μmol per panicle）. The lag time (time from the finishing of treatment to first floret opening) of male sterile line responsing to MeJA was 10 min shorter than that of male fertile line. MeJA induction on the rice floret opening could markedly be inhibited by SA solution at the concentration of 1 mmol /L, and the inhibition of SA on MeJA induction on floret opening of male-sterile rice could be nullified by MeJA treatment again. MeJA effect on floret opening of male-sterile rice could be clearly synergized by pretreatment with 24-epibras sinolide (24-epiBL).
