CAJ | 학술논문

建立液相色谱-质谱联用检测动物内脏(猪心、猪肝、猪肾、鸡心和鸡肝)和肌肉(猪里脊肉和鸡胸肉)组织中11种全氟化合物(PFCs)的分析方法.11种PFCs包括9种常见PFCs和2种调聚酸.首先考察对比了WAX和MAX两种固相萃取小柱对11种PFCs的回收情况,最终选用WAX柱对样品的萃取液进行净化.通过样品前处理方法的比较和优化,对动物内脏和肌肉组织分别采用离子对液液萃取和碱消解法.最后分析检测了农贸市场上的猪肝等多种实际样品,除了PFTA和2种调聚酸回收率较低外,其他8种PFCs的实际样品的加标回收都在80%～120%之间,方法检出限为0.002～0.032ng/g.
건립액상색보-질보련용검측동물내장(저심、저간、저신、계심화계간)화기육(저리척육화계흉육)조직중11충전불화합물(PFCs)적분석방법.11충PFCs포괄9충상견PFCs화2충조취산.수선고찰대비료WAX화MAX량충고상췌취소주대11충PFCs적회수정황,최종선용WAX주대양품적췌취액진행정화.통과양품전처리방법적비교화우화,대동물내장화기육조직분별채용리자대액액췌취화감소해법.최후분석검측료농무시장상적저간등다충실제양품,제료PFTA화2충조취산회수솔교저외,기타8충PFCs적실제양품적가표회수도재80%～120%지간,방법검출한위0.002～0.032ng/g.
A high performance liquid chromatography-electrospray ionization-tandem mass spectrometric(HPLC-ESI-MS/MS)method was proposed for the determination of 11 perfluorinated compounds(PFCs)including 9 common PFCs,2-perfluorooctyl ethanoic acid(FOEA)and 2H-PerfluorO_2~--decenoic acid(FOUEA)in animal visceral(pork heart,pork liver,pork kidney,chicken heart and chicken liver)and muscle(pork tenderloin and chicken breas0 tissues.WAX and MAX SPE cartridges were compared for the difference in recovery of 11 PFCs and WAX SPE cartridge was selected.Ion-pair liquid-liquid extraction and alkaline digestion were determined to be optimal methods for sample pretreatment of visceral and muscle tissues,respectively.In the determinaitons of real samples including pork liver,etc purchased from a local farmers' market,the developed method exhibited low spike recoveries of PFTA,FOEA and FOUEA,while the spike recoveries of other 8 PFCs were found to be in the range of 80%-120%.The limit of detection of the method ranged from 0.002 to 0.032 ng/g.