CAJ | 학술논문

目的:探讨在急性运动中骨骼肌线粒体能量代谢与线粒体移动相关基因mirol的表达变化特征.方法:以C57 BL/6小鼠一次中等强度负荷跑台运动(0°,13m/min)为实验模型,将小鼠随机分为5组:安静对照组、运动30min、60min、90min、120min组,分别测定骨骼肌线粒体呼吸控制比(RCR),ATP合成酶活性、骨骼肌H_2O_2生成量,并观察运动中骨骼肌mirol mRNA表达变化.结果:(1)120分钟急性运动过程中mirolmRNA表达均较安静组显著升高,其中E30、E60、E90、E120组分别较安静组增高32.8%、107.6%、63.8%及44.8%;(2)运动中各时间点骨骼肌H_2O_2均较安静组显著升高,E30～E120组分别较安静组升高22.1%、26.7%、37.6%、33.7%;(3)E30组ATP合成酶活性与安静组比较明显增加,其余各组无明显变化;整个运动过程中线粒体呼吸控制比无显著变化.结论:急性运动中骨骼肌mirol mRNA表达较安静时显著增加,可能利于促进线粒体呼吸和ATP合成,以应对细胞能量需求.
목적:탐토재급성운동중골격기선립체능량대사여선립체이동상관기인mirol적표체변화특정.방법:이C57 BL/6소서일차중등강도부하포태운동(0°,13m/min)위실험모형,장소서수궤분위5조:안정대조조、운동30min、60min、90min、120min조,분별측정골격기선립체호흡공제비(RCR),ATP합성매활성、골격기H_2O_2생성량,병관찰운동중골격기mirol mRNA표체변화.결과:(1)120분종급성운동과정중mirolmRNA표체균교안정조현저승고,기중E30、E60、E90、E120조분별교안정조증고32.8%、107.6%、63.8%급44.8%;(2)운동중각시간점골격기H_2O_2균교안정조현저승고,E30～E120조분별교안정조승고22.1%、26.7%、37.6%、33.7%;(3)E30조ATP합성매활성여안정조비교명현증가,기여각조무명현변화;정개운동과정중선립체호흡공제비무현저변화.결론:급성운동중골격기mirol mRNA표체교안정시현저증가,가능리우촉진선립체호흡화ATP합성,이응대세포능량수구.
Objective The purpose of this study was to investigate the expression of mitochondrial motility-related gene mirol and mitochondrial energy metabolism during an acute bout of prolonged exercise. Methods CS7 BL/6 mice underwent a moderate intensity treadmill running with the slope of 0° and at the speed of 13m/min, and they were randomly divided into 5 groups:resting group(R),exercise groups running for 30min(E30),60min(E60),90min (E90) and 120min(E120),respectively. Respiratory control index and ATP synthesis activity in isolated mitochondria were detected. Skeletal muscle H_2O_2 concentration,mirol mRNA expressions were also measured. Results (1)mirol mRNA contents were significantly increased in groups E30 to E120,as compared with the group R;and mirol mRNA expression increased 32.8%, 107.6%,63.8% and 44.8% respectively in groups E30 to E120. (2)H_2O_2 contents of skeletal muscle were clearly increased in group s E30 to E120. (3)ATP synthesis activity elevated at 30 min of exercise and returned to the baseline thereafter,whereas respiratory control index without remarkable change in groups E30 to E120. Conclusion Muscular mirol mRNA expression significantly increased during 120min of exercise as compared with the resting status and thus facilitated the mitochondrial respiratory and ATP synthesis for matching the energy demand during exercise.