CAJ | 학술논문

对豆皮采用热水浸提得到豆皮水溶性多糖,通过DEAE-cellulose离子交换柱洗脱,得到3个组分.本文主要研究了采用0.3 M NaOH溶液洗脱纯化得到的组分SHP-3的物化性质.对SHP-3进行凝胶渗透色谱、气相色谱分析、紫外光谱及高碘酸氧化-Smith降解分析,结果表明:SHP-3的分子量为45554,其糖醛酸含量为26.71%(wt.%),单糖组成摩尔比为Rah∶Fuc∶Ara∶Xyl∶Man∶Gal∶Glu=3.55∶0.44∶11.58∶1∶7.45∶5.12∶1.12,紫外光谱在260～280 nm没有吸收峰,表明没有蛋白质或核酸等物质;高碘酸氧化-Smith降解结果表明SHP-3的连接结构以为(1→4)糖苷键为主,其摩尔比例占68.9%,(1→2)糖苷键占11.4%,(1→6)糖苷键占19.7%.
대두피채용열수침제득도두피수용성다당,통과DEAE-cellulose리자교환주세탈,득도3개조분.본문주요연구료채용0.3 M NaOH용액세탈순화득도적조분SHP-3적물화성질.대SHP-3진행응효삼투색보、기상색보분석、자외광보급고전산양화-Smith강해분석,결과표명:SHP-3적분자량위45554,기당철산함량위26.71%(wt.%),단당조성마이비위Rah∶Fuc∶Ara∶Xyl∶Man∶Gal∶Glu=3.55∶0.44∶11.58∶1∶7.45∶5.12∶1.12,자외광보재260～280 nm몰유흡수봉,표명몰유단백질혹핵산등물질;고전산양화-Smith강해결과표명SHP-3적련접결구이위(1→4)당감건위주,기마이비례점68.9%,(1→2)당감건점11.4%,(1→6)당감건점19.7%.
The water-soluble polysaccharide of soybean hull (SHP) was extracted by hot water and three fractions were obtained by using DEAE-cellulose anion exchange column.The physicochemical properties of the purified fractions SHP3,eluted with 0.3 M NaOH,were investigated.Gel permeation chromatography showed that the molecule weight of SHPviolet spectra indicated that there was no presence of protein in SHP-3.The molar percentages of (1→2) glycosidic linkage,(1→4) glycosidic linkage and (1→6) glycosidic linkage were 11.4%,68.9% and 19.7%,respectively.