CAJ | 학술논문

目的评价间接免疫荧光法(IIF)检测血清中抗细胞膜DNA(cmDNA)抗体诊断系统性红斑狼疮(SLE)的价值.方法检索1970-2009年PubMed、中国期刊全文数据库,检索词为:系统性红斑狼疮、SLE、自身抗体、细胞膜DNA,收集关于抗cmDNA抗体诊断SLE价值的相关研究文献,根据Whiting等制定的QUADAS文献质量评价工具对文献进行质量评价,使用MetaDisc1.4进行异质性分析、Meta分析及绘制SROC曲线.结果共纳入文献7篇,合计2447例,其中经金标准确认的SLE患者共824例.Meta分析异质性检验P=0.0009,I2=73.5%;合并敏感度为69.0%,95%CI为66.0%～72.0%;合并特异度为97.0%,95%CI为96.0%～98.0%;合并阳性似然比+LR=21.37,95%CI为12.17～37.54;合并阴性似然比-LR=0.28,95%CI为0.18～0.45;SROC曲线下面积AUC=0.933 6(SE=0.060 7).结论目前间接免疫荧光法检测血清中抗cmDNA抗体诊断SLE有一定的价值,可作为诊断SLE的特异性指标.
목적 평개간접면역형광법(IIF)검측혈청중항세포막DNA(cmDNA)항체진단계통성홍반랑창(SLE)적개치.방법 검색1970-2009년PubMed、중국기간전문수거고,검색사위:계통성홍반랑창、SLE、자신항체、세포막DNA,수집관우항cmDNA항체진단SLE개치적상관연구문헌,근거Whiting등제정적QUADAS문헌질량평개공구대문헌진행질량평개,사용MetaDisc1.4진행이질성분석、Meta분석급회제SROC곡선.결과 공납입문헌7편,합계2447례,기중경금표준학인적SLE환자공824례.Meta분석이질성검험P=0.0009,I2=73.5%;합병민감도위69.0%,95%CI위66.0%～72.0%;합병특이도위97.0%,95%CI위96.0%～98.0%;합병양성사연비+LR=21.37,95%CI위12.17～37.54;합병음성사연비-LR=0.28,95%CI위0.18～0.45;SROC곡선하면적AUC=0.933 6(SE=0.060 7).결론목전간접면역형광법검측혈청중항cmDNA항체진단SLE유일정적개치,가작위진단SLE적특이성지표.
Objective To evaluation the signifacance of anti- cell membrane associated DNA ( cmDNA ) antibodies in systemic lupus erythematosus (SLE ) by indirect immunofluorescence (IIF ). Methods We searched PubMed and full-text data base of Chinese periodical to identify studies on the diagnostic value of anti-cell membrane associated DNA antibody in patients with SLE. The searching time was from 1970 to 2009. The key words are systemic lupus erythematosus , SLE and autoantibody. Select those valid researches . Performed a systematic reviem of outcomes about laboratory diagnosis for SLE by using Meta-discl.40. MetaDisc was used to analyze heterogeneity, perform meta-analyses and draw sum mary receiver operator characteristic curve ( SROC).Results The resultes of meta-analysis showed that there was high heterogeneity in the 7 researches , the sensitivity and specificity was 69.0% and 97.0%; the positive likelihood ( +LR ) and negative likelihood ( -LR) was 21.37and 0.28 ; the area under the curve ( AUC ) was 0.9336. Conclunion The autoantibodies to cmDNA by IIF can be used as a special index in diagnosing the SLE.