中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2012年
4期
423-425
,共3页
二异丙酚%肌细胞,平滑肌%气管%钙%离子
二異丙酚%肌細胞,平滑肌%氣管%鈣%離子
이이병분%기세포,평활기%기관%개%리자
Propofol%Myocytes,smooth muscle%Trachea%Calcium%Ions
目的 评价异丙酚对兔离体气管平滑肌细胞内游离钙离子浓度([Ca2+]i)的影响.方法 采用急性酶分离方法分离兔气管平滑肌细胞,采用随机数字表法,将细胞随机分为3组(n=5):异丙酚组(Ⅰ组,终浓度300 μmol/L)、异丙酚(终浓度300 μmol/L)+2-氨乙基硼酸二苯酯(终浓度40μmol/L)(Ⅱ组)和异丙酚(300 μmol/L)+斯里兰卡肉桂碱(终浓度10 μmol/L)(Ⅲ组).Ⅰ组加入终浓度300 μmol/L的异丙酚,孵育15 min后,用无钙的Hank平衡盐溶液冲洗3次,加入1μmol/L乙酰胆碱,记录[Ca2+]i.Ⅱ组加入终浓度40μmol/L的2-氨乙基硼酸二苯酯孵育15 min后,再加入终浓度为300μmol/L的异丙酚,与2-氨乙基硼酸二苯酯共同孵育15 min后,用无钙的Hank平衡盐溶液冲洗3次,加入1 μmol/L的乙酰胆碱.Ⅲ组加入终浓度10 μmol/L的斯里兰卡肉桂碱孵育15 min后,再加入终浓度为300μmol/L的异丙酚,与斯里兰卡肉桂碱共同孵育15 min后,用无钙的Hank平衡盐溶液冲洗3次,再加入1 μmol/L的乙酰胆碱.通过负荷钙离子荧光指示剂Fluo-3/AM测定气管平滑肌细胞内[Ca2+]i.结果 与Ⅰ组比较,Ⅱ组气管平滑肌细胞内[Ca2+]i差异无统计学意义(P＞0.05),Ⅲ组[Ca2+]i明显降低(P＜0.05).结论 异丙酚可降低兔离体气管平滑肌细胞内[Ca2+]i,其机制可能与抑制内质网1,4,5-三磷酸肌醇通路有关,而与内质网兰诺定通路无关.
目的 評價異丙酚對兔離體氣管平滑肌細胞內遊離鈣離子濃度([Ca2+]i)的影響.方法 採用急性酶分離方法分離兔氣管平滑肌細胞,採用隨機數字錶法,將細胞隨機分為3組(n=5):異丙酚組(Ⅰ組,終濃度300 μmol/L)、異丙酚(終濃度300 μmol/L)+2-氨乙基硼痠二苯酯(終濃度40μmol/L)(Ⅱ組)和異丙酚(300 μmol/L)+斯裏蘭卡肉桂堿(終濃度10 μmol/L)(Ⅲ組).Ⅰ組加入終濃度300 μmol/L的異丙酚,孵育15 min後,用無鈣的Hank平衡鹽溶液遲洗3次,加入1μmol/L乙酰膽堿,記錄[Ca2+]i.Ⅱ組加入終濃度40μmol/L的2-氨乙基硼痠二苯酯孵育15 min後,再加入終濃度為300μmol/L的異丙酚,與2-氨乙基硼痠二苯酯共同孵育15 min後,用無鈣的Hank平衡鹽溶液遲洗3次,加入1 μmol/L的乙酰膽堿.Ⅲ組加入終濃度10 μmol/L的斯裏蘭卡肉桂堿孵育15 min後,再加入終濃度為300μmol/L的異丙酚,與斯裏蘭卡肉桂堿共同孵育15 min後,用無鈣的Hank平衡鹽溶液遲洗3次,再加入1 μmol/L的乙酰膽堿.通過負荷鈣離子熒光指示劑Fluo-3/AM測定氣管平滑肌細胞內[Ca2+]i.結果 與Ⅰ組比較,Ⅱ組氣管平滑肌細胞內[Ca2+]i差異無統計學意義(P＞0.05),Ⅲ組[Ca2+]i明顯降低(P＜0.05).結論 異丙酚可降低兔離體氣管平滑肌細胞內[Ca2+]i,其機製可能與抑製內質網1,4,5-三燐痠肌醇通路有關,而與內質網蘭諾定通路無關.
목적 평개이병분대토리체기관평활기세포내유리개리자농도([Ca2+]i)적영향.방법 채용급성매분리방법분리토기관평활기세포,채용수궤수자표법,장세포수궤분위3조(n=5):이병분조(Ⅰ조,종농도300 μmol/L)、이병분(종농도300 μmol/L)+2-안을기붕산이분지(종농도40μmol/L)(Ⅱ조)화이병분(300 μmol/L)+사리란잡육계감(종농도10 μmol/L)(Ⅲ조).Ⅰ조가입종농도300 μmol/L적이병분,부육15 min후,용무개적Hank평형염용액충세3차,가입1μmol/L을선담감,기록[Ca2+]i.Ⅱ조가입종농도40μmol/L적2-안을기붕산이분지부육15 min후,재가입종농도위300μmol/L적이병분,여2-안을기붕산이분지공동부육15 min후,용무개적Hank평형염용액충세3차,가입1 μmol/L적을선담감.Ⅲ조가입종농도10 μmol/L적사리란잡육계감부육15 min후,재가입종농도위300μmol/L적이병분,여사리란잡육계감공동부육15 min후,용무개적Hank평형염용액충세3차,재가입1 μmol/L적을선담감.통과부하개리자형광지시제Fluo-3/AM측정기관평활기세포내[Ca2+]i.결과 여Ⅰ조비교,Ⅱ조기관평활기세포내[Ca2+]i차이무통계학의의(P＞0.05),Ⅲ조[Ca2+]i명현강저(P＜0.05).결론 이병분가강저토리체기관평활기세포내[Ca2+]i,기궤제가능여억제내질망1,4,5-삼린산기순통로유관,이여내질망란낙정통로무관.
Objective To investigate the effect of propofol on the intracellular calcium ion concentration ([ Ca2+ ]i) in isolated rabbit tracheal smooth muscle cells (TSMCs).Methods The single rabbit TSMC was isolated by acute enzymatic isolation method as described by Cheng et al.The isolated TSMCs were randomly divided into 3 groups ( n =5 each):propofol group (group Ⅰ ),propofol + 2-aminoethoxy-diphenylborate (2-APB) group (group Ⅱ ) and propofol + the blocker ryanodine group (group Ⅲ).In group Ⅰ,the cells were incubated with propofol with the final concentration of 300 μmol/L for 15 min,followed by washing with calcium-free Hank's balanced salt solution (HBSS) for 3 times,acetylcholine 1 μmol/L was then added to the culture medium and [ Ca2+ ]i was recorded.In group Ⅱ,the cells were incubated with 2-APB with the final concentration of 40 μmol/L for 15 min,propofol with the final concentration of 300 μmol/L was then added,the cells were incubated with 2-APB and propofol for another 15 min,followed bywashing with calcium-free HBSS for 3 times,and acetylcholine 1 μmol/L was then added.In group Ⅲ,the cells were incubated with ryanodine with the final concentration of 10 μmol/L for 15 min,propofol with the final concentration of 300 μmol/L was then added,the cells were incubated with ryanodine and propofol for another 15 min,followed by washing with calcium-free HBSS for 3 times,and acetylcholine 1 μmol/L was then added.[ Ca2+ ]i in TSMCs was measured using the fluorescent Ca2+ indicator fluor-3/AM.Results Compared with group Ⅰ,no significant change was found in [Ca2+ ]i in group Ⅱ (P＞0.05),while [Ca2+ ]i was significantly decreased in group Ⅲ (P＜0.05).Conclusion Propofol can decrease the [Ca2+ ]i in isolated rabbit TSMCs,and the mechanism may be related to inhibition of inositol 1,4,5-trisphosphate pathway,but not ryanodine pathway.
