CAJ | 학술논문

目的初步探讨HBV基因S区的准种特点与乙型肝炎病毒(HBV)感染临床转归的关系.方法选择慢性HBV携带者、慢性乙型肝炎患者、慢性重型肝炎患者各3例,所有患者均为男性,且HBV基因型均为C型.扩增患者血清中HBV基因S区片段并克隆,每份样本挑选20个克隆进行测序,并用SPSS 15.0软件进行统计分析.结果慢性HBV携带者以及慢性乙型肝炎患者的HBV基因S区准种复杂度小于慢性重型乙型肝炎患者,但差异无统计学意义(t=1.7,P=0.26).对于HBV S区T细胞表位的第45、47和85位氨基酸,慢性乙型肝炎患者的准种构成情况比HBV携带者复杂(P=0.01),与慢性重型乙型肝炎的差别无统计学意义(P=0.06),计算机模拟分析提示优势克隆和非优势克隆的T细胞表位均能与CTL受体有效结合.结论 HBV基因S区部分T细胞表位的准种构成差异可能与HBV慢性感染的临床转归相关.
목적 초보탐토HBV기인S구적준충특점여을형간염병독(HBV)감염림상전귀적관계.방법 선택만성HBV휴대자、만성을형간염환자、만성중형간염환자각3례,소유환자균위남성,차HBV기인형균위C형.확증환자혈청중HBV기인S구편단병극륭,매빈양본도선20개극륭진행측서,병용SPSS 15.0연건진행통계분석.결과 만성HBV휴대자이급만성을형간염환자적HBV기인S구준충복잡도소우만성중형을형간염환자,단차이무통계학의의(t=1.7,P=0.26).대우HBV S구T세포표위적제45、47화85위안기산,만성을형간염환자적준충구성정황비HBV휴대자복잡(P=0.01),여만성중형을형간염적차별무통계학의의(P=0.06),계산궤모의분석제시우세극륭화비우세극륭적T세포표위균능여CTL수체유효결합.결론 HBV기인S구부분T세포표위적준충구성차이가능여HBV만성감염적림상전귀상관.
Objective To investigate the association of hepatitis B virus(HBV) S gene quasispecies with the outcome of HBV infection.Methods Serum samples were collected from three chronic HBV carriers, three chronic hepatitis B and three chronic severe hepatitis B patients.All subjects were male and with HBV genotype C.HBV S gene was amplified, and 20 clones of HBV fragment were randomly selected and sequenced from each sample.SPSS 15.0 software was adopted for analysis.Results Quasispecies complexity of HBV S gene in chronic HBV carriers and chronic hepatitis B tended lower than that of the severe chronic hepatitis B, but the difference was not of statistical significance (P＞0.05).In T cell epitope 45, 47, 85 amino acid sites of the HBV S gene, the constitution of quasispecies in the chronic hepatitis B was more complex than that of the HBV carriers (P=0.01), but compared with the severe chronic hepatitis, the difference was not significant (P=0.06).The computer model showed that both the dominant clones and the non dominant clones could effectively bind to the receptors of cytotoxic T lymphocytes.Conclusion Quasispecies in some T cell epitopes of HBV S gene may be related with the clinical outcome of hepatitis B.