CAJ | 학술논문

目的探讨舒芬太尼后处理对大鼠缺血再灌注时心肌细胞凋亡的影响.方法健康雄性SD大鼠36只,体重250 ～ 280 g,采用随机数字表法,将大鼠随机分为3组(n=12):假手术组(S组)、缺血再灌注组(I/R组)和舒芬太尼后处理组(SP组).采用结扎左冠状动脉30 min,再灌注120min的方法制备心肌缺血再灌注模型.S组只挂线不结扎左冠状动脉；SP组于再灌注即刻静脉注射舒芬太尼3.0 μg/kg.于缺血再灌注期间记录HR和MAP.于再灌注120 min时,处死大鼠,取心脏,测定心肌梗死面积,采用RT-PCR测定心肌Bax mRNA和Bcl-2 mRNA的表达,采用TUNEL法检测心肌凋亡细胞,计算凋亡指数.结果三大鼠各时点HR比较差异无统计学意义(P＞0.05)；与S组比较,I/R组心肌缺血再灌注期间MAP降低(P＜0.05),SP组差异无统计学意义(P＞0.05),I/R组和SP组凋亡指数和Bax mRNA表达水平升高,I/R组Bcl-2 mRNA表达水平降低,SP组Bcl-2 mRNA表达水平升高(P＜0.05)；与I/R组比较,SP组心肌梗死面积、凋亡指数和Bax mRNA表达水平降低,Bcl-2 n.RNA表达水平升高(P＜0.05).结论舒芬太尼后处理可能通过上调Bcl-2表达,下调Bax表达,抑制细胞凋亡,从而减轻大鼠心肌缺血再灌注损伤.
목적 탐토서분태니후처리대대서결혈재관주시심기세포조망적영향.방법 건강웅성SD대서36지,체중250 ～ 280 g,채용수궤수자표법,장대서수궤분위3조(n=12):가수술조(S조)、결혈재관주조(I/R조)화서분태니후처리조(SP조).채용결찰좌관상동맥30 min,재관주120min적방법제비심기결혈재관주모형.S조지괘선불결찰좌관상동맥；SP조우재관주즉각정맥주사서분태니3.0 μg/kg.우결혈재관주기간기록HR화MAP.우재관주120 min시,처사대서,취심장,측정심기경사면적,채용RT-PCR측정심기Bax mRNA화Bcl-2 mRNA적표체,채용TUNEL법검측심기조망세포,계산조망지수.결과 삼대서각시점HR비교차이무통계학의의(P＞0.05)；여S조비교,I/R조심기결혈재관주기간MAP강저(P＜0.05),SP조차이무통계학의의(P＞0.05),I/R조화SP조조망지수화Bax mRNA표체수평승고,I/R조Bcl-2 mRNA표체수평강저,SP조Bcl-2 mRNA표체수평승고(P＜0.05)；여I/R조비교,SP조심기경사면적、조망지수화Bax mRNA표체수평강저,Bcl-2 n.RNA표체수평승고(P＜0.05).결론 서분태니후처리가능통과상조Bcl-2표체,하조Bax표체,억제세포조망,종이감경대서심기결혈재관주손상.
Objective To investigate the effect of sufentanil postconditioning on cardiomyocyte apoptosis during myocardial ischemia-reperfusion (I/R) in rats.Methods Thirty-six male Sprague-Dawley rats weighing 250-280 g were randomly divided into 3 groups ( n =12 each):sham operation group (group S),I/R group and sufentanil postconditioning group (group SP).Myocardial I/R was induced by occlusion of the left coronary artery for 30 min followed by 120 min reperfusion.Sufentanil 3.0μg/kg was injected iv at the beginning of reperfusion in group SP.HR and MAP were recorded during I/R.The rats were sacrificed at 120 min of reperfusion and their hearts were removed for determination of infarct size,number of apoptotic cardiomyocytes,and the expression of Bax mRNA and Bcl-2 mRNA,and apoptotic index was caculated.Results There was no significant difference in HR among the 3 groups ( P ＞ 0.05 ).Compared with group S,MAP and Bcl-2 mRNA expression were significantly decreased,apoptotic index and Bax mRNA expression increased in group I/R,and apoptotic index,and the expression of Bax mRNA and Bcl-2 mRNA were increased in group SP (P ＜ 0.05).Compared with group I/R,the infarct size,apoptotic index and Bax mRNA expression were decreased,and Bcl-2 mRNA expression was increased in group SP (P ＜ 0.05).Conclusion Sufentanil postconditioning can attenuate myocardial I/R injury in rats by up-regulating Bcl-2 expression,down-regulating Bax expression and inhibitting cardiomyocyte apoptosis.