中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2012年
6期
496-503
,共8页
吴小闲%卢耀增%陈颂%潘菊华%王阶%黄世敬%赖春辉%郭卫中%孙丽华%徐艳峰%鲍琳琳%卢葳
吳小閒%盧耀增%陳頌%潘菊華%王階%黃世敬%賴春輝%郭衛中%孫麗華%徐豔峰%鮑琳琳%盧葳
오소한%로요증%진송%반국화%왕계%황세경%뢰춘휘%곽위중%손려화%서염봉%포림림%로위
快速进展型%一般进展型%精英控制者%猴免疫缺陷病毒%记忆细胞亚群
快速進展型%一般進展型%精英控製者%猴免疫缺陷病毒%記憶細胞亞群
쾌속진전형%일반진전형%정영공제자%후면역결함병독%기억세포아군
Rapid progressor(RP)%Normal progressor(NP)%Elite controllor (EC)%Simian immunodeficiency virus(SIV)%Memory cell subset
目的 中国恒河猴感染猴免疫缺陷病毒(SIV)后,根据其病毒载量水平、疾病进程速度等,可分为普通进展型(NP)、快速进展型(RP)以及长期不进展型(LTNP)/精英控制者(EC)等3类.对属于不同进展类型的动物的各项参数进行比较,有助于进一步理解AIDS的发病机制.方法 使用SIVmac239毒株静脉感染中国恒河猴后,定期采血进行血液学、免疫学、病毒学及病理学检查；并对各参数进行比较.结果 16只感染动物中,有1例动物(RM449猴)快速进展并死亡于感染后4.5个月(RP型)；2例(RM450和RM453)的血浆病毒载量被控制至低于检测水平(EC型)；其余13例属于NP型.与13只NP型的猴相比较,RM449( RP型)病毒载量高,SIV特异IgG低,效应记忆型CD4+T亚群细胞数低,且流式图出现类似“分化阻滞”的现象；外周血B细胞数的降幅大,以组织样B细胞和活化的记忆B细胞为主；淋巴组织耗竭,胸腺消失；具更高的抗淋巴组织的自身抗体的水平.而RM450和RM453猴(EC型)大致与之相反.结论 AIDS的形成可能与T、B淋巴细胞亚群的分化和功能不足有关；而胸腺、淋巴组织等的结构破坏,可能是其病理学基础.
目的 中國恆河猴感染猴免疫缺陷病毒(SIV)後,根據其病毒載量水平、疾病進程速度等,可分為普通進展型(NP)、快速進展型(RP)以及長期不進展型(LTNP)/精英控製者(EC)等3類.對屬于不同進展類型的動物的各項參數進行比較,有助于進一步理解AIDS的髮病機製.方法 使用SIVmac239毒株靜脈感染中國恆河猴後,定期採血進行血液學、免疫學、病毒學及病理學檢查；併對各參數進行比較.結果 16隻感染動物中,有1例動物(RM449猴)快速進展併死亡于感染後4.5箇月(RP型)；2例(RM450和RM453)的血漿病毒載量被控製至低于檢測水平(EC型)；其餘13例屬于NP型.與13隻NP型的猴相比較,RM449( RP型)病毒載量高,SIV特異IgG低,效應記憶型CD4+T亞群細胞數低,且流式圖齣現類似“分化阻滯”的現象；外週血B細胞數的降幅大,以組織樣B細胞和活化的記憶B細胞為主；淋巴組織耗竭,胸腺消失；具更高的抗淋巴組織的自身抗體的水平.而RM450和RM453猴(EC型)大緻與之相反.結論 AIDS的形成可能與T、B淋巴細胞亞群的分化和功能不足有關；而胸腺、淋巴組織等的結構破壞,可能是其病理學基礎.
목적 중국항하후감염후면역결함병독(SIV)후,근거기병독재량수평、질병진정속도등,가분위보통진전형(NP)、쾌속진전형(RP)이급장기불진전형(LTNP)/정영공제자(EC)등3류.대속우불동진전류형적동물적각항삼수진행비교,유조우진일보리해AIDS적발병궤제.방법 사용SIVmac239독주정맥감염중국항하후후,정기채혈진행혈액학、면역학、병독학급병이학검사；병대각삼수진행비교.결과 16지감염동물중,유1례동물(RM449후)쾌속진전병사망우감염후4.5개월(RP형)；2례(RM450화RM453)적혈장병독재량피공제지저우검측수평(EC형)；기여13례속우NP형.여13지NP형적후상비교,RM449( RP형)병독재량고,SIV특이IgG저,효응기억형CD4+T아군세포수저,차류식도출현유사“분화조체”적현상；외주혈B세포수적강폭대,이조직양B세포화활화적기억B세포위주；림파조직모갈,흉선소실；구경고적항림파조직적자신항체적수평.이RM450화RM453후(EC형)대치여지상반.결론 AIDS적형성가능여T、B림파세포아군적분화화공능불족유관；이흉선、림파조직등적결구파배,가능시기병이학기출.
Objective To compare the bio-medical parameters in SIV infected Chinese rhesus monkeys with diverse disease progression,by which the pathogenesis of simian AIDS were to be investigated.Methods Sixteen Chinese rhesus monkeys were inoculated intravenously with SIVmac239 and followed-up for 18 months.Based on their progression patterns and plasma viral loads,animals were divided into 3 groups,including 1 rapid progressor( RP),13 normal progressors(NP),and 2 elite controllor(EC).Their parameters of haematology,virology,immunology and pathology were examined and compared. Results Compared with other animals,RM449(RP) showed higher viral load,unresponsive humoral immunity,and higher level of auto-antibodies against lymph node,thymus,and spleen.Additionally,its effector memory CD4 count was lower,with the transformation progress being "blocked-like" from naive/central memory subsets to effector memory subset,as the flow-cytometry assay showed.Notable decrease in its peripheral B cell was also observed,especially to the sub-population of tissue-like memory B cells and activated memory B cells.Pathological examination showed the depletion of lymphoid tissue,atrophy of spleen and loss of thymus.Moreover,most of these parameters of RM450 and RM453 (EC) changed opposite to that of RP.Conclusion The hallmarks of RM449 were higher viraemia and lower SIV specific IgG level,which may due to the disturbance of T cells and B cells development and differentiation.Moreover,destructions of organs of the immune system may contribute to the disturbance.Our study suggest that the change of micro-environments of thymus induced by SIV infection,which is necessary in T cell and B cell development and differentiation,may contribute at least partially to the AIDS pathogenesis.
