CAJ | 학술논문

目的通过检测系统性红斑狼疮(SLE)、狼疮肾炎(LN)患者血清抗nRNP、抗Sm、抗dsDNA、ANCA及ANuA等自身抗体,探讨几种自身抗体在狼疮肾炎患者中的临床意义.方法狼疮肾炎患者55例,非狼疮肾炎的SLE患者60例为对照组,血清抗dsDNA、ANCA采用间接免疫荧光法测定,ANCA间接免疫荧光法阳性者用免疫印迹法进一步确认,抗nRNP、抗Sm及ANuA采用欧蒙斑点法测定.结果两组病程差异有统计学意义(P＜0.001),LN发病率为SLE患者的47.8%,抗nRNP及抗Sm阳性率LN组较对照组高,但无统计学意义(P=0.395、P=0.483);LN组抗dsDNA、ANCA及ANuA阳性率较对照组高且差异有统计学意义(P=0.028、P=0.024,P＜0.001).结论 SLE病程是合并LN的重要因素之一,血清抗dsDNA、ANCA及ANuA等自身抗体检测均有助于LN的诊断.
목적 통과검측계통성홍반랑창(SLE)、랑창신염(LN)환자혈청항nRNP、항Sm、항dsDNA、ANCA급ANuA등자신항체,탐토궤충자신항체재랑창신염환자중적림상의의.방법 랑창신염환자55례,비랑창신염적SLE환자60례위대조조,혈청항dsDNA、ANCA채용간접면역형광법측정,ANCA간접면역형광법양성자용면역인적법진일보학인,항nRNP、항Sm급ANuA채용구몽반점법측정.결과 량조병정차이유통계학의의(P＜0.001),LN발병솔위SLE환자적47.8%,항nRNP급항Sm양성솔LN조교대조조고,단무통계학의의(P=0.395、P=0.483);LN조항dsDNA、ANCA급ANuA양성솔교대조조고차차이유통계학의의(P=0.028、P=0.024,P＜0.001).결론 SLE병정시합병LN적중요인소지일,혈청항dsDNA、ANCA급ANuA등자신항체검측균유조우LN적진단.
Objective To assess the clinical significance of several autoantibodies including antiribonucleoprotein(anti-nRNP),anti-Smith antibody(anti-Sin),anti-double stranded DNA(anti-dsDNA),anti-neutrophil cytoplasmic antibody(ANCA)and anti-nucleosome antibody(ANuA)in patients with lupus nephritis.Methods One hundred and fifteen patients with SLE were divided into lupus nephritis group(n=55)and non-lupus nephritis control group(n=60).Serum anti-dsDNA and ANCA were measured with indirect immunofluorescence assay(IFL),and IFL positive result for ANCA was confirmed by Western blotting.Anti-Sm and ANuA were detected with Euroassay.Results There was statistical difference of disease course between the two groups(P＜0.001).There weren't significant difference of the positive rates of anti-nRNP and anti-Sm between the two groups(P=0.395,P=0.483 respectively).The positive rates of anti-dsDNA,ANCA and ANuA were much higher in lupus nephritis group than those of control group(P=0.028,P=0.024 respectively,both P ＜0.001).Conclusion We speculate that disease duration of SLE might play an important role in LN.Detection of serum anti-dsDNA,ANCA and AnuA contributes to the diagnosis of lupus nephritis.