CAJ | 학술논문

背景:近年来临床遗传学和分子生物学研究均表明,瘢痕疙瘩的形成与遗传具有密切的关系.但增生性瘢痕与遗传是否有关,目前尚未明确.目的:了解增生性瘢痕与瘢痕疙瘩在遗传学改变上的异同.设计、时间及地点;对比观察,实验于2007-03/2008-12在广东医学院完成.材料:瘢痕标本均来自2003-01/2008-12广东医学院附属医院整形外科门诊及住院患者16例,其中增生性瘢痕10例,男3例,女7例,年龄20～50岁;瘢痕疙瘩6例,男1例,女5例,年龄19～46岁.方法:提取瘢痕疙瘩及增生性瘢痕组织DNA,应用比较基因组杂交技术观察增生性瘢痕及瘢痕疙瘩基因组的不平衡即遗传物质的丢失或扩增情况,比较两者间DNA拷贝数变化的差异.主要观察指标:①两组DNA拷贝数的缺失率的比较.②两组DNA拷贝数的扩增率的比较.结果:增生性瘢痕组未发现特异区域的DNA拷贝数的高频率缺失或扩增;瘢痕疙瘩组出现高频率的DNA拷贝数缺失的染色体是1,16,20号及22号染色体,未发现特异区域的DNA拷贝数的高频率扩增.两组1,16,20,22染色体DNA拷贝数的缺失率相比较,瘢痕疙瘩组明显高于增生性瘢痕组(P<0.05).结论:与瘢痕疙瘩相比,增生性瘢痕不存在明显的DNA拷贝数缺失或扩增,增生性瘢痕的形成与发展可能与遗传没有直接的关系.
배경:근년래림상유전학화분자생물학연구균표명,반흔흘탑적형성여유전구유밀절적관계.단증생성반흔여유전시부유관,목전상미명학.목적:료해증생성반흔여반흔흘탑재유전학개변상적이동.설계、시간급지점;대비관찰,실험우2007-03/2008-12재엄동의학원완성.재료:반흔표본균래자2003-01/2008-12엄동의학원부속의원정형외과문진급주원환자16례,기중증생성반흔10례,남3례,녀7례,년령20～50세;반흔흘탑6례,남1례,녀5례,년령19～46세.방법:제취반흔흘탑급증생성반흔조직DNA,응용비교기인조잡교기술관찰증생성반흔급반흔흘탑기인조적불평형즉유전물질적주실혹확증정황,비교량자간DNA고패수변화적차이.주요관찰지표:①량조DNA고패수적결실솔적비교.②량조DNA고패수적확증솔적비교.결과:증생성반흔조미발현특이구역적DNA고패수적고빈솔결실혹확증;반흔흘탑조출현고빈솔적DNA고패수결실적염색체시1,16,20호급22호염색체,미발현특이구역적DNA고패수적고빈솔확증.량조1,16,20,22염색체DNA고패수적결실솔상비교,반흔흘탑조명현고우증생성반흔조(P<0.05).결론:여반흔흘탑상비,증생성반흔불존재명현적DNA고패수결실혹확증,증생성반흔적형성여발전가능여유전몰유직접적관계.
BACKGROUND: Clinical genetics and molecular biology studies have shown that the occurrence and development of the keloid is closely related to the inheritance. However, it remians unclear if the same is ture to the hypertrophic scar. OBJECTIVE: To investigate similadties and differences of genetic alteration between the hyperplastic scar and the keloid, DESIGN, TIME AND SETTING: A contrast observational experiment was performed in Guangdong Medical College between March 2007 and December 2008.MATERIALS: Scar samples were taken from 16 patients (in-patient and out-patient) in the Department of Plastic Surgery, the Affiliated Hospital of Guangdong Medical College, with10 patients with hypertrophic scars (3 males and 7 females, 20-50 years old) and 6 patients with keloids (1 males and 5 females, 19-46 years old). METHODS: The DNA of both hyperplastic scar and keloid tissues was extracted to investigate, using comparative genomic hybridization technique, the genomic imbalance (the lose or amplification of genetic material), so as to make a comparative study on differences of the DNA copy number changes between the two. RESULTS: Neither altofrequent loss nor amplification of DNA copy number was found in any specific DNA region of hyperplastic scar tissues; as for the keloid, special DNA altofrequent loss regions were also not found, but altofrequent DNA copy number loss regions presented in 1, 16, 20 and 22 chromosomes. Comparatively, the keloid presented much higher loss rate of the DNA copy number in 1,16,20 and 22 chromosomes than the hyperplastic scar (P < 0.05).CONCLUSION: The hyperplastic scar has no conspicuous DNA copy number lose or amplification compared with the keloid, which indicates that the occurrence and development of the hyperplastic scar may not have any direct relation with the inheritance.