CAJ | 학술논문

[目的]建立一种用低龄日本大耳白兔制备高效价迟钝爱德华氏菌(Edwardsiella tarda, Et)免疫血清的方法.[方法]先制备迟钝爱德华氏菌(Et)抗原.用日本大耳白兔作免疫动物,分2组试验,一组是2月龄组(T组),另一组是6月龄(S组).采取耳缘静脉注射的方法,小剂量连续多次（第1、7、14、21、27、33、41天时免疫剂量分别为0.2、0.2、0.25、0.2、0.25、0.3、0.4 ml）免疫日本大耳白兔.免疫后,收集免疫血清,然后用微量凝集反应法分2次试血（第1次是免疫第33天后的第7天,采用1:20抗血清;第2次是免疫第41天时,采用1:40抗血清）,测定免疫血清抗体效价.[结果]凝集反应显示,在免疫剂量相同的情况下,第1次试血测Et免疫血清效价,2月龄组T2血清效价最高为1:640,6月龄组S1、S2和S4血清效价均达1:1 280,显示6月龄组的体液免疫应答反应强于2月龄组;经继续免疫8 d后,第2次试血测Et免疫血清效价可见,2月龄组的T1、T2、T3和6月龄组的S1、S2、S4免疫血清效价一样,均高达1:5 120.表明此时在接受相同剂量Et抗原刺激后,2月龄组和6月龄组的体液免疫应答反应已趋相同.[结论]试验中所采用的T组动物（日本大耳白兔）月龄较小,免疫后仍产生了与S组一致的高效价抗血清,说明用低龄日本大耳白兔制备高效价Et免疫血清的方法可行.
[목적]건립일충용저령일본대이백토제비고효개지둔애덕화씨균(Edwardsiella tarda, Et)면역혈청적방법.[방법]선제비지둔애덕화씨균(Et)항원.용일본대이백토작면역동물,분2조시험,일조시2월령조(T조),령일조시6월령(S조).채취이연정맥주사적방법,소제량련속다차（제1、7、14、21、27、33、41천시면역제량분별위0.2、0.2、0.25、0.2、0.25、0.3、0.4 ml）면역일본대이백토.면역후,수집면역혈청,연후용미량응집반응법분2차시혈（제1차시면역제33천후적제7천,채용1:20항혈청;제2차시면역제41천시,채용1:40항혈청）,측정면역혈청항체효개.[결과]응집반응현시,재면역제량상동적정황하,제1차시혈측Et면역혈청효개,2월령조T2혈청효개최고위1:640,6월령조S1、S2화S4혈청효개균체1:1 280,현시6월령조적체액면역응답반응강우2월령조;경계속면역8 d후,제2차시혈측Et면역혈청효개가견,2월령조적T1、T2、T3화6월령조적S1、S2、S4면역혈청효개일양,균고체1:5 120.표명차시재접수상동제량Et항원자격후,2월령조화6월령조적체액면역응답반응이추상동.[결론]시험중소채용적T조동물（일본대이백토）월령교소,면역후잉산생료여S조일치적고효개항혈청,설명용저령일본대이백토제비고효개Et면역혈청적방법가행.
[Objective] The study aimed to establish an approach to high titer Et immune serum preparation by low-aged Japanese rabbits. [Method] Antigen of Et was prepared at first, Japanese rabbits were taken as immunized animal, and divided into two groups for experiments, one is two-month-old group (T group), and another is six-month-old group (S group). Japanese rabbits were continuous by immunized with low-dose by using auricular intravenous method, then immune sera were collected. Immune serum antibody titer was determined with micro-agglutination reaction method. [Result] Agglutination reaction showed that the Et serum titer of S group is higher than that of T group in the first testing with the same dose. But in the second testing, the serum titer of the T group and and the S group was consistent. [Conclusion] The animals in the T group (Japanese rabbits) were fewer months old, and produced high titer antiserum was which consistent with the S group, which indicated that the method of preparing of high titer Et immune serum by low-aged Japanese rabbits was feasible.