CAJ | 학술논문

磷酸烯醇式丙酮酸羧化酶(PEPCase)是控制植物籽粒中蛋白质/油脂含量比例的关键酶.本研究利用RT-PCR技术,克隆PEPCase基因的cDNA片段,并将克隆的PEPCase基因片段反向连接替代植物表达载体PBI121的GUS基因.从花生栽培品种荔蒲大花生中克隆获得编码PEPCase基因的cDNA片段(886 bp),测序结果显示其核苷酸序列与已报道的花生(EU391629)、棉花(AY008939)、大豆(D10717)、拟南芥(AY210895)、豌豆(D64037)PEPCase基因对应部分的同源性分别为99.77%、84.37%、81.54%、81.25%、78.71%.构建的反义表达载体中PEPCase基因由35S启动子所控制,将构建的反义表达载体命名为pBGPEP.为通过反义抑制技术提高花生含油量提供了基因及表达载体.
린산희순식병동산최화매(PEPCase)시공제식물자립중단백질/유지함량비례적관건매.본연구이용RT-PCR기술,극륭PEPCase기인적cDNA편단,병장극륭적PEPCase기인편단반향련접체대식물표체재체PBI121적GUS기인.종화생재배품충려포대화생중극륭획득편마PEPCase기인적cDNA편단(886 bp),측서결과현시기핵감산서렬여이보도적화생(EU391629)、면화(AY008939)、대두(D10717)、의남개(AY210895)、완두(D64037)PEPCase기인대응부분적동원성분별위99.77%、84.37%、81.54%、81.25%、78.71%.구건적반의표체재체중PEPCase기인유35S계동자소공제,장구건적반의표체재체명명위pBGPEP.위통과반의억제기술제고화생함유량제공료기인급표체재체.
Phosphoenolpyruvate carboxylase (PEPCase) plays an important role in the control of the ratio of the protein/lipid content in plant seed .A 886 bp DNA fragment was amplified by RT-PCR using PEPCase gene special primer from peanut seed.The DNA sequence analysis indicated that the sequence of this fragment was shared 99.77%、84.37%、81.54%、81.25%、78.71% homology with the corresponding sequence of the reported PEPCase gene in peanut、cotton、soybean、arabidopsis thaliana、pea.The 886 bp fragment digested by BamH I and SacI and ligated into the GUS site of the pBI121 in the antisense orientation and constructed the antisence expression vector pBGPEP.