CAJ | 학술논문

目的探讨扩增片段长度多态性(AFLP)技术应用于变态反应疾病等复杂样本的遗传研究之可能性,探讨差异片段后续分析方法.方法利用公共数据库中获得的遗传背景差异较大的成对材料进行AFLP筛选所得的差异片段,采用不同的BLAST程序进行深入的生物信息学分析.结果不同的BLAST程序获得的结果所包含的可用信息量不同.以BLASTX程式获得的结果显示,前述差异片段分别与洋葱伯克霍尔德菌噬菌体及结核分支杆菌末端酶的大亚基高度同源.在后二者基本信息缺失的情况下,利用关键词搜索获得了末端酶与肿瘤形成及转移的重要信息.结论通过采用AFLP方法对遗传背景差异较大的成对材料进行分子标记筛选,获得的差异片段进一步经生物信息学分析,对于后续的深入研究十分关键.
목적 탐토확증편단장도다태성(AFLP)기술응용우변태반응질병등복잡양본적유전연구지가능성,탐토차이편단후속분석방법.방법 이용공공수거고중획득적유전배경차이교대적성대재료진행AFLP사선소득적차이편단,채용불동적BLAST정서진행심입적생물신식학분석.결과 불동적BLAST정서획득적결과소포함적가용신식량불동.이BLASTX정식획득적결과현시,전술차이편단분별여양총백극곽이덕균서균체급결핵분지간균말단매적대아기고도동원.재후이자기본신식결실적정황하,이용관건사수색획득료말단매여종류형성급전이적중요신식.결론 통과채용AFLP방법대유전배경차이교대적성대재료진행분자표기사선,획득적차이편단진일보경생물신식학분석,대우후속적심입연구십분관건.
Objective To explore the application probability of AFLP method (Amplified Fragment Length Polymorphism) for the genetic study of complicated samples and to make clear the analytical approach on polymorphic fragments.Methods An AFLP fragement sequence,resolved between murines with high and low metastatic hepatocarcinoma,was retrieved from a reference paper and was annotated on NCBI database by different BLAST programs. Results The retrieved information varied along with the different BLAST programs, nevertheless BLASTX program provided more comprehensive information and showed that the differentiated fragment exhibited high homology with terminase large subunits from Burkholderia phage Bups phil and Mycobacterium tuberculosis H37Ra. However, there is no detailed information for the latter,key words search returned the relationship between terminase and the formation and metastasis of tumors and provided the further research clues. Conclusions Whole genome screening in parallel of AFLP markers between genetically differentiated paired materials would readily produce informatic fragments. Further analysis of the sequenced fragments by informatic methods would facilitate the subsequent research.