CAJ | 학술논문

目的探讨塞来昔布对慢性粒细胞白血病(CML)原代细胞bcr-abl融合基因的mRNA表达、p210蛋白表达和蛋白质酪氨酸激酶(PTK)活性的影响.方法以不同浓度的塞来昔布(0、10、20、40、80、160 μmol/L)分别干预CML原代细胞36 h,进行荧光实时定量反转录聚合酶链反应(RQ-RTPCR)、Western blotting和PTK活性检测.结果 80～160μmol/L的塞来昔布下调bcr-abl的mRNA表达;随着塞来昔布浓度增加,p210蛋白表达逐步下调;40μmol/L以上的塞来昔布能明显抑制PTK活性,但非浓度依耐性.结论塞来昔布能不同程度地下调CML原代细胞bcr-abl的mRNA和蛋白表达,并抑制PTK活性.
목적 탐토새래석포대만성립세포백혈병(CML)원대세포bcr-abl융합기인적mRNA표체、p210단백표체화단백질락안산격매(PTK)활성적영향.방법 이불동농도적새래석포(0、10、20、40、80、160 μmol/L)분별간예CML원대세포36 h,진행형광실시정량반전록취합매련반응(RQ-RTPCR)、Western blotting화PTK활성검측.결과 80～160μmol/L적새래석포하조bcr-abl적mRNA표체;수착새래석포농도증가,p210단백표체축보하조;40μmol/L이상적새래석포능명현억제PTK활성,단비농도의내성.결론 새래석포능불동정도지하조CML원대세포bcr-abl적mRNA화단백표체,병억제PTK활성.
Objective To explore the effects of celecoxib, a cyclooxygenase-2 (COX-2) specific inhibitor, on the mRNA expression protein expression and protein tyrosine kinase (PTK) activity of bcr-abl fusion gene in chronic myeloid leukemia (CML) primary cells. Methods The primary cells were incubated with various concentration of celecoxib (0, 10, 20, 40, 80, 160 μmol/L) for 36 hours, then the changes of mRNA expression, p210 expression and PTK activity of bcr-abl fusion gene in each group were examined respectively by real time quantitative reverse transcriptase polymerase chain reaction (RQ-RT-PCR), Western blotting and PTK activity analysis. Results The mRNA expression was downregulated with high concentration of celecoxib (80-160 μmol/L), and p210 expression was decreased gradually after increasing celecoxib. The PTK activity was inhibited in a concentration-independent way, and the statistic difference was observed only above 40μmol/L concentration of celecoxib. Conclusion Celecoxib can downregulate mRNA,and the protein expression of bcr-abl fusion gene; and inhibit the PTK activity by defferent extent.