CAJ | 학술논문

目的探讨慢性非细菌性前列腺炎/慢性骨盆疼痛综合征(CAP/CPPS)患者外周血CD4+ CDhigh25调节性T细胞数量和功能的变化及其临床意义.方法采用流式细胞术检测45例CAP/CPPS患者和18例健康对照者外周血CD4+ CDhigh25T细胞、CD4+ CDhigh25T细胞分别占CD4+ T淋巴细胞的百分比,ELISA法检测血清和精浆IL-6、IL-10、TGF-β1、TNF-α水平,Spearman法分析各检测指标与临床症状的相关性.结果 CAP/CPPS患者外周血CD4+ CD+25和CD4+ CDhigh25T细胞占CD:T淋巴细胞的百分比[(16.94±9.07)%,(5.98±0.57)%]与正常对照组[(15.98±3.76)%,(6.93±0.49)%]比较差异无统计学意义(P>0.05);与正常对照组比较,CAP/CPPS患者血清中TNF-α水平增加[(0.34±0.01)ng/ml与(0.11±0.01)ng/ml,P<0.053,TGF-β1水平降低[(15.17±6.35)ng/ml与(27.01±13.29)ng/ml,P<0.05],精浆中IL-6、TGF-β1及TNF-α水平增高[(0.80±0.01)ng/ml与(0.44±0.11)ng/ml,(195.07±20.46)ng/ml与(126.46±25.99)ng/ml,(0.30±0.02)ng/ml与(0.12±0.02)ng/ml,P<0.05];血清和精浆IL-6水平与美国国立卫生研究院慢性前列腺炎症状评分指数(NIH-CPSI)呈正相关(r=0.456,P<0.05;r=0.506,P<0.01),精浆IL-10水平与疼痛指数呈正相关(r=0.276,P<0.05);CD4+ CDhigh25 T细胞数与血清TGF-～1水平呈正相关(r-0.486,P<0.01),与患者年龄、病程及NIH-CPSI指数未见明显相关性(r=-0.075,P>0.05;r-=0.506,P>0.05;r=-0.112,P>0.05).结论外周血CD4+ CDhigh25 T调节细胞数量与CAP/CPPS的发病无明显关联,其功能缺陷可能导致CAP/CPPS发病,细胞因子在CAP/CPPS的发病过程中起重要作用,并且与临床症状密切相关.
목적 탐토만성비세균성전렬선염/만성골분동통종합정(CAP/CPPS)환자외주혈CD4+ CDhigh25조절성T세포수량화공능적변화급기림상의의.방법 채용류식세포술검측45례CAP/CPPS환자화18례건강대조자외주혈CD4+ CDhigh25T세포、CD4+ CDhigh25T세포분별점CD4+ T림파세포적백분비,ELISA법검측혈청화정장IL-6、IL-10、TGF-β1、TNF-α수평,Spearman법분석각검측지표여림상증상적상관성.결과 CAP/CPPS환자외주혈CD4+ CD+25화CD4+ CDhigh25T세포점CD:T림파세포적백분비[(16.94±9.07)%,(5.98±0.57)%]여정상대조조[(15.98±3.76)%,(6.93±0.49)%]비교차이무통계학의의(P>0.05);여정상대조조비교,CAP/CPPS환자혈청중TNF-α수평증가[(0.34±0.01)ng/ml여(0.11±0.01)ng/ml,P<0.053,TGF-β1수평강저[(15.17±6.35)ng/ml여(27.01±13.29)ng/ml,P<0.05],정장중IL-6、TGF-β1급TNF-α수평증고[(0.80±0.01)ng/ml여(0.44±0.11)ng/ml,(195.07±20.46)ng/ml여(126.46±25.99)ng/ml,(0.30±0.02)ng/ml여(0.12±0.02)ng/ml,P<0.05];혈청화정장IL-6수평여미국국립위생연구원만성전렬선염증상평분지수(NIH-CPSI)정정상관(r=0.456,P<0.05;r=0.506,P<0.01),정장IL-10수평여동통지수정정상관(r=0.276,P<0.05);CD4+ CDhigh25 T세포수여혈청TGF-～1수평정정상관(r-0.486,P<0.01),여환자년령、병정급NIH-CPSI지수미견명현상관성(r=-0.075,P>0.05;r-=0.506,P>0.05;r=-0.112,P>0.05).결론 외주혈CD4+ CDhigh25 T조절세포수량여CAP/CPPS적발병무명현관련,기공능결함가능도치CAP/CPPS발병,세포인자재CAP/CPPS적발병과정중기중요작용,병차여림상증상밀절상관.
Objective To explore the role of CD4+ CDhigh25 regulatory T cells in the pathogenesis of chronic abacterial prostatitis/chronic pelvic pain syndrome (CAP/CPPS).Methods The percentage of CD4+ CD+25 and CD4+ CDhigh25 regulatory T cells was detected by flow cytometry from 45 CAP/CPPS pa-tients and 18 normal controls.The levels of interleukin-6(IL-6),IL-10,tumor necrosis factor-α(TNF-α) and transforming growth factor-β1 (TGF-β1) in serum and seminal plasma were measured by ELISA in the same cohort.Results There was no significant difference in the percentage of peripheral blood CD4+CD+25 and CD4+Cdhigh+ cells between CAP/CPPS patients and normal control (P>0.05).The ser-CD4+CD+25 and CD4+Cdhigh+ cells between CAP/CPPS patients and normal control (P>0.05>.The ser-um levels of TGF-β1 in patients with CAP/CPPS were markedly lower than those in controls (P<0.05),serum TNF-α and seminal plasma IL-6,TGF-β1 and TNF-α in CAP/CPPS patients were markedly higher than those in controls (P<0.05).There was a positive correlation between the IL-6 and the NIH-CPSI.There was also a positive correlation between the IL-10 and the pain index.In ad-dition,the percentage of peripheral blood CD+4CDhigh25 cells was positively correlated with serum TGF-β1.But the percentage of CD+4CDhigh25 cells had no correlation with ages,duration of CAP/CPPS pa-tients,NIH-CPSI and the other cytokines.Conclusions The defective function of peripheral blood CD+4CD+25 regulatory T cells may be related with the pathogenesis of CAP/CPPS.The cytokines may also play an important role in the process of pathogenesis of CAP/CPPS.