中华泌尿外科杂志
中華泌尿外科雜誌
중화비뇨외과잡지
CHINESE JOURNAL OF UROLOGY
2008年
5期
346-349
,共4页
宣强%杨小丽%莫林键%黄逢雨%覃敏%何敏%庞友红%莫曾南
宣彊%楊小麗%莫林鍵%黃逢雨%覃敏%何敏%龐友紅%莫曾南
선강%양소려%막림건%황봉우%담민%하민%방우홍%막증남
前列腺肿瘤%良性前列腺增生%组织激肽释放酶
前列腺腫瘤%良性前列腺增生%組織激肽釋放酶
전렬선종류%량성전렬선증생%조직격태석방매
Prostatic neoplasms%Benign prostatic hyperplasia%Kallikrein
目的 探讨组织激肽释放酶基因7(KLK7)在不同前列腺组织中的表达情况.方法运用逆转录聚合酶链反应法检测正常前列腺(5例)、良性前列腺增生(BPH)及BPH细胞株(BPH1,13例)、前列腺癌及前列腺癌细胞株(8例)的上皮细胞中KLK7mRNA表达水平;蛋白质印迹法检测不同前列腺组织上皮细胞中KLK7蛋白表达水平;免疫组化分析正常前列腺(20例)、BPH(50例)、前列腺癌(103例)组织中KLK表达水平.根据染色强度分为4个等级(-,+,++,+++)进行半定量分析,染色强度++及+++者判定为阳性.结果 正常组、BPH组和前列腺癌组KLK7 mRNA表达相对值分别为0.59、0.52、0.02,组间比较差异有统计学意义(F=13.03,P<0.01),前列腺癌上皮中KLK7 mRNA表达下调(P<0.01),正常前列腺和BPH上皮中KLK7 mRNA表达差异无统计学意义(P>0.05).KLK7蛋白在正常前列腺、增生前列腺、DU145、LNCaP、PC3、22RV1、BPH细胞株中表达水平相对值分别为0.22、0.40、0.01、0.05、0、0.03、0.14.免疫组化染色结果 显示正常前列腺组织、BPH组织、前列腺癌中KLK7蛋白表达阳性率分别为65.0%(13/20)、76.0%(38/50)、17.5%(18/103),前列腺癌组与前2组比较差异均有统计学意义(P<0.01),前2组间比较差异无统计学意义(P>0.05).结论 KLK7在前列腺癌组织中表达下调,提示KLK7在前列癌的发生和进展中可能起一定作用.
目的 探討組織激肽釋放酶基因7(KLK7)在不同前列腺組織中的錶達情況.方法運用逆轉錄聚閤酶鏈反應法檢測正常前列腺(5例)、良性前列腺增生(BPH)及BPH細胞株(BPH1,13例)、前列腺癌及前列腺癌細胞株(8例)的上皮細胞中KLK7mRNA錶達水平;蛋白質印跡法檢測不同前列腺組織上皮細胞中KLK7蛋白錶達水平;免疫組化分析正常前列腺(20例)、BPH(50例)、前列腺癌(103例)組織中KLK錶達水平.根據染色彊度分為4箇等級(-,+,++,+++)進行半定量分析,染色彊度++及+++者判定為暘性.結果 正常組、BPH組和前列腺癌組KLK7 mRNA錶達相對值分彆為0.59、0.52、0.02,組間比較差異有統計學意義(F=13.03,P<0.01),前列腺癌上皮中KLK7 mRNA錶達下調(P<0.01),正常前列腺和BPH上皮中KLK7 mRNA錶達差異無統計學意義(P>0.05).KLK7蛋白在正常前列腺、增生前列腺、DU145、LNCaP、PC3、22RV1、BPH細胞株中錶達水平相對值分彆為0.22、0.40、0.01、0.05、0、0.03、0.14.免疫組化染色結果 顯示正常前列腺組織、BPH組織、前列腺癌中KLK7蛋白錶達暘性率分彆為65.0%(13/20)、76.0%(38/50)、17.5%(18/103),前列腺癌組與前2組比較差異均有統計學意義(P<0.01),前2組間比較差異無統計學意義(P>0.05).結論 KLK7在前列腺癌組織中錶達下調,提示KLK7在前列癌的髮生和進展中可能起一定作用.
목적 탐토조직격태석방매기인7(KLK7)재불동전렬선조직중적표체정황.방법운용역전록취합매련반응법검측정상전렬선(5례)、량성전렬선증생(BPH)급BPH세포주(BPH1,13례)、전렬선암급전렬선암세포주(8례)적상피세포중KLK7mRNA표체수평;단백질인적법검측불동전렬선조직상피세포중KLK7단백표체수평;면역조화분석정상전렬선(20례)、BPH(50례)、전렬선암(103례)조직중KLK표체수평.근거염색강도분위4개등급(-,+,++,+++)진행반정량분석,염색강도++급+++자판정위양성.결과 정상조、BPH조화전렬선암조KLK7 mRNA표체상대치분별위0.59、0.52、0.02,조간비교차이유통계학의의(F=13.03,P<0.01),전렬선암상피중KLK7 mRNA표체하조(P<0.01),정상전렬선화BPH상피중KLK7 mRNA표체차이무통계학의의(P>0.05).KLK7단백재정상전렬선、증생전렬선、DU145、LNCaP、PC3、22RV1、BPH세포주중표체수평상대치분별위0.22、0.40、0.01、0.05、0、0.03、0.14.면역조화염색결과 현시정상전렬선조직、BPH조직、전렬선암중KLK7단백표체양성솔분별위65.0%(13/20)、76.0%(38/50)、17.5%(18/103),전렬선암조여전2조비교차이균유통계학의의(P<0.01),전2조간비교차이무통계학의의(P>0.05).결론 KLK7재전렬선암조직중표체하조,제시KLK7재전렬암적발생화진전중가능기일정작용.
Objective To study the expression of kallikrein 7 (KLK7) in different prostate tissues and its clinical significance. Methods KLK7 mRNA levels in normal prostate epithelia (5 cases), benign prostat(ic) hyperplasia (BPH) epithelia (13 cases), prostate cancer and prostate cancer cell lines (8 cases) were analyzed by using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). Western blot was used to analyze the protein levels of human kallikrein 7 (hK7) in benign prostate epithelia and prostate cancer cell lines, hK7 expressions were examined in 20 normal prostate tissue specimens, 50 BPH specimens and 103 prostate cancer specimens by immunohistochemical staining.Results The mRNA levels of KLK7 in normal prostate, BPH and prostate cancer were 0.59, 0.52 and 0.02 respectively, mRNA levels of KLK7 were significantly different among the three groups (F=13.03, P<0.01). mRNA levels of KLK7 were decreased in prostate cancers compared with that in benign hyperplastic prostate epithelial cells (P<0.01) and in normal prostate epithelial cells (P<0.01). No significant difference of KLK7 mRNA levels was found between normal prostate and BPH. The protein levels of KLK7 in normal prostate, BPH, DU145, LNCaP, PC3,22RV1 and BPH1 was 0.22, O. 40, 0.01, 0.05, 0, 0.03 and 0.14 respectively, hK7 protein level was down-regulated in prostate cancer cell lines compared to benign prostate epithelial cells. The expression of bK7 was observed in benign prostate epithelial cells, whereas little or no staining was observed in prostate cancer cells in immunohistochemical study, hK7 protein was detected in 13 of 20 (65%)normal prostate specimens, 38 of 50 (76%) BPH specimens and 18 of 103 (17.5%) prostate cancer specimens. The difference between the normal prostate and prostate cancer was significant (Z=-4.43, P<0.01). The difference between BPH and prostate cancer was significant (Z=-7.77,P<0.01) as well. However, no significant difference of hK7 protein level was found between normal prostate and BPH (Z=-1. 52, P>0.05). Conclusions KLK7 expression level is down regulated in prostate cancer. KLK7 may play an important role in prostate cancer progression.