CAJ | 학술논문

目的研制一种用纳米免疫磁珠层析技术用以检测HIV抗体的快速诊断试剂.方法运用碳二亚(EDC)将重组的HIV抗原gp41、gp36偶联到200 nm的超顺磁纳米颗粒上,在硝酸纤维素(NC)膜上包被gp41、gp36抗原,制备成免疫层析检测卡,然后对检测卡进行性能分析评估.结果对HIV抗体国家参考品血清盘(胶体金类)检测,符合要求;对20份HIV抗体阳性和600份抗体阴性临床血清检测,灵敏度为100%,特异性为98.5%.检测卡室温保存12个月性能稳定.结论研制出一种纳米免疫磁珠HIV抗体检测试剂,具有检测简便、快速、稳定性好和适于现场检测等特点.
목적 연제일충용납미면역자주층석기술용이검측HIV항체적쾌속진단시제.방법 운용탄이아(EDC)장중조적HIV항원gp41、gp36우련도200 nm적초순자납미과립상,재초산섬유소(NC)막상포피gp41、gp36항원,제비성면역층석검측잡,연후대검측잡진행성능분석평고.결과 대HIV항체국가삼고품혈청반(효체금류)검측,부합요구;대20빈HIV항체양성화600빈항체음성림상혈청검측,령민도위100%,특이성위98.5%.검측잡실온보존12개월성능은정.결론 연제출일충납미면역자주HIV항체검측시제,구유검측간편、쾌속、은정성호화괄우현장검측등특점.
Objective To develop a rapid test kit for antibody to HIV by nano immunomagnetic lateral flow method.Methods A rapid test kit was developed by conjugation of the HIV antigen gp41 and gp36 to 200nm super paramagnetic particles by carbodiimide (EDC) and coating of the HIV antigen sp41 and gp36 to nitrocellulose membrane.Then the kit was evaluated with serials of experiments.Results The kit was qualified with examination of national reference panel of anti-HIV antibody for colloidal gold diagnostic kit.The sensitivity was 100% by tested with 20 HIV antibody positive sera,the specificity was 98.5% by tested with 600 HIV antibody negative sera,respectively.The stabilitv of the kit was over 12 month by storage at room temperature.Conclusion A diagnostic kit for antibody to HIV was developed with the advantages of convenience,rapid test,good stability and point of care.