中华消化杂志
中華消化雜誌
중화소화잡지
Chinese Journal of Digestion
2012年
8期
543-548
,共6页
乔春萍%施建平%叶慧英%高仁里%顾国妹%何峻峰%陈岳祥
喬春萍%施建平%葉慧英%高仁裏%顧國妹%何峻峰%陳嶽祥
교춘평%시건평%협혜영%고인리%고국매%하준봉%진악상
肝疾病,酒精性%生物碱类%肿瘤坏死因子α%白细胞介素6%转化生长因子β1
肝疾病,酒精性%生物堿類%腫瘤壞死因子α%白細胞介素6%轉化生長因子β1
간질병,주정성%생물감류%종류배사인자α%백세포개소6%전화생장인자β1
Liver diseases,alcoholic%Alkaloids%Tumor necrosis factor-alpha%Interleukin-6%Transforming growth factor betal
目的 探讨槐果碱对大鼠酒精性肝病(ALD)的疗效及其对TNF-α、转化生长因子-β1( TGF-β1)和IL-6表达的影响.方法 48只SD大鼠均分为健康组、模型组、预防组、治疗组,健康组每日予0.9％ NaCl溶液灌胃12周,模型组、预防组、治疗组大鼠予乙醇灌胃造模12周,预防组同时注射槐果碱20 mg·kg-1·d-1共12周,治疗组第5周起连续8周注射槐果碱20 mg·kg1·d-1.评价肝组织学病变,检测ALT、AST、碱性磷酸酶(AKP)、三酰甘油(TG)和总胆固醇(TC)含量,免疫组织化学和实时定量PCR检测肝组织TNF-α、IL-6和TGF-β1 mRNA和蛋白表达.多组间比较采用单因素方差分析,两两比较采用最小显著差法(LSD法),等级资料用Kruskal-Wallis H检验,多组两两比较采用Nemenyi法检验.结果 治疗组和预防组与模型组比较,大鼠肝脏脂肪变和炎性细胞浸润程度明显改善,ALT[(41.40±10.53)U/L和(40.75±6.94)U/L比(58.37±5.35)U/L]、AST[(121.60±16.24)U/L和(109.50±9.23)U/L比(156.63±32.47)U/L]、AKP[(114.88±40.37) U/L和(112.60±44.34) U/L比(161.75±28.95)U/L]、TC[(4.19±0.99)mmol/L和(2.69±1.35)mmol/L比(4.50±0.99)mmol/L]、TG[(1.48±0.28)mmol/L和(1.43±0.19) mmol/L比(1.67±0.20)mmol/L]水平明显下降,差异有统计学意义(P值均＜0.05).模型组大鼠肝组织中TNF-α、IL-6、TGF-β1的mRNA和蛋白表达水平较健康组、预防组、治疗组明显增高.槐果碱干预后,治疗组和预防组的TNF-α mRNA和蛋白表达水平[mRNA:1.36±0.08,1.16±0.05;蛋白:(3.38±0.82)％,(1.74±0.65)％]、IL-6 mRNA和蛋白表达水平[mRNA:1.51±0.05,1.39±0.02；蛋白:(5.89±0.96)％,(4.26±0.53)％]、TGF-β1 mRNA和蛋白表达水平[mRNA:1.39±0.04,1.37±0.02;蛋白:(4.27±0.97)％,(2.11±0.83)％]较模型组[mRNA:1.81±0.16,1.95±0.13,1.84±0.22;蛋白:(5.82±1.21)％,(7.63±1.03)％,(5.33±1.12)％]明显降低,差异有统计学意义(P值均＜0.01).结论 槐果碱能明显减轻乙醇导致的大鼠肝损伤,改善大鼠肝组织脂肪变和炎性反应程度,可能与其下调ALD大鼠肝组织TNF-α、IL-6和TGF-β1的表达有关.
目的 探討槐果堿對大鼠酒精性肝病(ALD)的療效及其對TNF-α、轉化生長因子-β1( TGF-β1)和IL-6錶達的影響.方法 48隻SD大鼠均分為健康組、模型組、預防組、治療組,健康組每日予0.9％ NaCl溶液灌胃12週,模型組、預防組、治療組大鼠予乙醇灌胃造模12週,預防組同時註射槐果堿20 mg·kg-1·d-1共12週,治療組第5週起連續8週註射槐果堿20 mg·kg1·d-1.評價肝組織學病變,檢測ALT、AST、堿性燐痠酶(AKP)、三酰甘油(TG)和總膽固醇(TC)含量,免疫組織化學和實時定量PCR檢測肝組織TNF-α、IL-6和TGF-β1 mRNA和蛋白錶達.多組間比較採用單因素方差分析,兩兩比較採用最小顯著差法(LSD法),等級資料用Kruskal-Wallis H檢驗,多組兩兩比較採用Nemenyi法檢驗.結果 治療組和預防組與模型組比較,大鼠肝髒脂肪變和炎性細胞浸潤程度明顯改善,ALT[(41.40±10.53)U/L和(40.75±6.94)U/L比(58.37±5.35)U/L]、AST[(121.60±16.24)U/L和(109.50±9.23)U/L比(156.63±32.47)U/L]、AKP[(114.88±40.37) U/L和(112.60±44.34) U/L比(161.75±28.95)U/L]、TC[(4.19±0.99)mmol/L和(2.69±1.35)mmol/L比(4.50±0.99)mmol/L]、TG[(1.48±0.28)mmol/L和(1.43±0.19) mmol/L比(1.67±0.20)mmol/L]水平明顯下降,差異有統計學意義(P值均＜0.05).模型組大鼠肝組織中TNF-α、IL-6、TGF-β1的mRNA和蛋白錶達水平較健康組、預防組、治療組明顯增高.槐果堿榦預後,治療組和預防組的TNF-α mRNA和蛋白錶達水平[mRNA:1.36±0.08,1.16±0.05;蛋白:(3.38±0.82)％,(1.74±0.65)％]、IL-6 mRNA和蛋白錶達水平[mRNA:1.51±0.05,1.39±0.02；蛋白:(5.89±0.96)％,(4.26±0.53)％]、TGF-β1 mRNA和蛋白錶達水平[mRNA:1.39±0.04,1.37±0.02;蛋白:(4.27±0.97)％,(2.11±0.83)％]較模型組[mRNA:1.81±0.16,1.95±0.13,1.84±0.22;蛋白:(5.82±1.21)％,(7.63±1.03)％,(5.33±1.12)％]明顯降低,差異有統計學意義(P值均＜0.01).結論 槐果堿能明顯減輕乙醇導緻的大鼠肝損傷,改善大鼠肝組織脂肪變和炎性反應程度,可能與其下調ALD大鼠肝組織TNF-α、IL-6和TGF-β1的錶達有關.
목적 탐토괴과감대대서주정성간병(ALD)적료효급기대TNF-α、전화생장인자-β1( TGF-β1)화IL-6표체적영향.방법 48지SD대서균분위건강조、모형조、예방조、치료조,건강조매일여0.9％ NaCl용액관위12주,모형조、예방조、치료조대서여을순관위조모12주,예방조동시주사괴과감20 mg·kg-1·d-1공12주,치료조제5주기련속8주주사괴과감20 mg·kg1·d-1.평개간조직학병변,검측ALT、AST、감성린산매(AKP)、삼선감유(TG)화총담고순(TC)함량,면역조직화학화실시정량PCR검측간조직TNF-α、IL-6화TGF-β1 mRNA화단백표체.다조간비교채용단인소방차분석,량량비교채용최소현저차법(LSD법),등급자료용Kruskal-Wallis H검험,다조량량비교채용Nemenyi법검험.결과 치료조화예방조여모형조비교,대서간장지방변화염성세포침윤정도명현개선,ALT[(41.40±10.53)U/L화(40.75±6.94)U/L비(58.37±5.35)U/L]、AST[(121.60±16.24)U/L화(109.50±9.23)U/L비(156.63±32.47)U/L]、AKP[(114.88±40.37) U/L화(112.60±44.34) U/L비(161.75±28.95)U/L]、TC[(4.19±0.99)mmol/L화(2.69±1.35)mmol/L비(4.50±0.99)mmol/L]、TG[(1.48±0.28)mmol/L화(1.43±0.19) mmol/L비(1.67±0.20)mmol/L]수평명현하강,차이유통계학의의(P치균＜0.05).모형조대서간조직중TNF-α、IL-6、TGF-β1적mRNA화단백표체수평교건강조、예방조、치료조명현증고.괴과감간예후,치료조화예방조적TNF-α mRNA화단백표체수평[mRNA:1.36±0.08,1.16±0.05;단백:(3.38±0.82)％,(1.74±0.65)％]、IL-6 mRNA화단백표체수평[mRNA:1.51±0.05,1.39±0.02；단백:(5.89±0.96)％,(4.26±0.53)％]、TGF-β1 mRNA화단백표체수평[mRNA:1.39±0.04,1.37±0.02;단백:(4.27±0.97)％,(2.11±0.83)％]교모형조[mRNA:1.81±0.16,1.95±0.13,1.84±0.22;단백:(5.82±1.21)％,(7.63±1.03)％,(5.33±1.12)％]명현강저,차이유통계학의의(P치균＜0.01).결론 괴과감능명현감경을순도치적대서간손상,개선대서간조직지방변화염성반응정도,가능여기하조ALD대서간조직TNF-α、IL-6화TGF-β1적표체유관.
Objective To investigate the efficacy of sophocarpine in rats with alcoholic liver disease and its effects on the expression of tumor necrosis factor (TNF)-α,interleukin (IL)-6 and transforming growth factor (TGF)-β1.Methods A total of 48 male Sprague-Dawley adult rats were evenly divided into healthy control group,model group,prevention group and treatment group.The rats in the healthy control group were gavaged with 0.9％NaCl every day for 12 weeks.The rats in the model group,prevention group and treatment group were gavaged with alcohol for 12 weeks to establish the model.The prevention group was injected with 20 mg · kg1 · d1 sophocarpine for 12 weeks.Since the fifth week,the treatment group was continuously injected with 20 mg · kg1 · d-1 sophocarpine for eight weeks.The histological changes were evaluated.The serum levels of alanine aminotransferase (ALT), aspartate aminotransferase ( AST ), alkaline phosphatase (AKP),triglyceride (TG) and total cholesterol (TC) were examined.And the expression of TNF-α,IL-6 and TGF-β1 in liver tissue at mRNA and protein level were detected with immunohistochemistry and real-time polymerase chain reaction (PCR) assay.Comparison among groups was perform with single factor analysis of variance,pairwise comparisons with least significant difference method (LSD method),ranked data with Kruskal-Wallis H-test and multiple pairwise comparison with Nemenyi test.Results Compared with model group,hepatic steatosis and inflammatory cell infiltration were significantly improved in the treatment group and prevention group.The levels of ALT (41.40 U/L± 10.53 U/L and 40.75 U/L±6.94 U/L vs 58.37 U/I±5.35 U/L),AST(121.60 U/L±16.24 U/L and 109.50 U/L±9.23 U/L vs 156.63 U/L±32.47 U/L),AKP(114.88 U/L±40.37 U/L and 112.60 U/L±44.34 U/L vs 161.75 U/L±28.95 U/L),TG (4.19 mmol/L±0.99 mmol/L and 2.69 mmol/L± 1.35 mmol/L vs 4.50 mmol/L±0.99 mmol/L) and TC (1.48 mmol/L±0.28 mmol/L and 1.43 mmol/L±0.19 mmol/L vs 1.67 mmol/L±0.20 mmol/L) significantly decreased and the difference was statistically significant ( all P＜0.05).The expression of TNF-α,IL-6 and TGF-β1 at mRNA and protein level in liver tissue of model group were significantly higher than those of healthy control group,prevention group and treatment group.After treated with sophocarpine,the expression of TNF-α(mRNA:1.36 ± 0.08,1.16 ± 0.05 ; protein:3.38 ％ ± 0.82 ％,1.74 ％ ± 0.65 ％ ),IL-6 (mRNA:1.51 ± 0.05,1.39 ± 0.02; protein:5.89％ ± 0.96％,4.26％ ± 0.53％) and TGF-β1 (mRNA:1.39±0.04,1.37±0.02; protein:4.27％ ±0.97％,2.11％ ±0.83％) of treatment group and prevention group at mRNA and protein level significantly lower than those of model group (mRNA:1.81±0.16,1.95 ±0.13,1.84±0.22; protein:5.82％ ± 1.21％,7.63％ ±1.03％,5.33％± 1.12％) and the difference was statistically significant (all P＜0.01).Conclusion Sophocarpine significantly alleviates alcohol induced liver injury in rats,improves liver steatosis and inflammatory reaction degree,which may be related with the downregulation of TNF-α,TGF-β1 and IL-6 expression in liver tissue of ALD rats.
