CAJ | 학술논문

葛仙米(N.sphaeroides)的产量和产地面积逐年减少,这可能与当地广泛使用除草剂草甘膦有关.为此,本文测定了不同浓度(0.15、0.30、0.45、0.6mmol/L)的草甘膦处理的葛仙米的颗粒大小、干重、叶绿素荧光、叶绿素浓度.所有测量参数与浓度和时间显著相关:0.15mmol/L处理组的颗粒直径较对照组小15%(2d后);叶绿素a浓度和最大量子产率(Fv/Fm)在最高浓度组(0.6mmol/L)4d后开始受到影响;第8天,相对生长速率(以干重计算,大于0.15mmol/L)、光合作用活性(大于0.3mmol/L)均受显著抑制,其中0.15mmol/L处理组相对生长下降60%,而更高浓度处理组出现负生长,0.6mmol/L组藻体漂白死亡.结合实验结果,文章讨论了草甘膦的毒性机理及其对葛仙米(N.sphaeroides)的潜在影响.
갈선미(N.sphaeroides)적산량화산지면적축년감소,저가능여당지엄범사용제초제초감련유관.위차,본문측정료불동농도(0.15、0.30、0.45、0.6mmol/L)적초감련처리적갈선미적과립대소、간중、협록소형광、협록소농도.소유측량삼수여농도화시간현저상관:0.15mmol/L처리조적과립직경교대조조소15%(2d후);협록소a농도화최대양자산솔(Fv/Fm)재최고농도조(0.6mmol/L)4d후개시수도영향;제8천,상대생장속솔(이간중계산,대우0.15mmol/L)、광합작용활성(대우0.3mmol/L)균수현저억제,기중0.15mmol/L처리조상대생장하강60%,이경고농도처리조출현부생장,0.6mmol/L조조체표백사망.결합실험결과,문장토론료초감련적독성궤리급기대갈선미(N.sphaeroides)적잠재영향.
The productivity of Nostoc sphaeroides,an edible cyanobacterium found in paddy rice fields has declined in recent years. It may relate to the increased application of agricultural herbicides. To assess the impact of glyphosate exposure(0.15,0.30,0.45 and 0.6 mmol/L Gly acid) ,the effects on colony size,dry biomass accumulation,chlorophyll a fluorescence (Fv/Fm) and chlorophyll a biosynthesis were investigated over an 8d period. All parameters were significantly inhibited in a concentration used and time dependent way. After 2d exposure to 0. 15 mmol/L Gly colonies were approximately 15% smaller than the controls. After 4d exposure,chlorophyll a content and Fv/Fm were affected by the highest concentration used(0.6 mmol/L Gly). By the 8d, chlorophyll biosynthesis and Fv/Fm were significantly inhibited by concentrations greater than 0.15 and 0.3 mmol/L Gly, respectively. The 8d relative growth rates ( RGRs), calculated for dry biomass, were significantly affected by all glyphosate treatments,there was a 60% reduction at 0.15 mmol/L Gly and negative RGRs at higher concentrations indicate a loss of biomass. Exposure to 0. 6 mmol/L Gly was lethal with loss of colony integrity,bleaching of pigments and no photosynthetic activity by 8d. These results are discussed in terms of the mechanisms of toxicity and the potential implications for the long term sustainability of the N. sphaeroides resource.