CAJ | 학술논문

建立了微乳液毛细管电动色谱-激光诱导荧光法测定用NBD-Cl衍生后的肾上腺素和多巴胺的方法.微乳液母液的组成为w(SDS):w(1-丁醇):2(n-庚烷):w(水)=3.31:6.61:0.80:89.28.优化后的分离条件为:20 mmol/L硼酸钠+体积分数为20%的微乳液+体积分数为4%的乙腈(调pH至9.3)组成缓冲液,分离电压20 kV,峰面积和分析物浓度之间有良好的线性关系.应用本方法测定2种中药和风湿性心脏病患者血浆中的肾上腺索和多巴胺,回收率为95.8%～105.6%.
건립료미유액모세관전동색보-격광유도형광법측정용NBD-Cl연생후적신상선소화다파알적방법.미유액모액적조성위w(SDS):w(1-정순):2(n-경완):w(수)=3.31:6.61:0.80:89.28.우화후적분리조건위:20 mmol/L붕산납+체적분수위20%적미유액+체적분수위4%적을정(조pH지9.3)조성완충액,분리전압20 kV,봉면적화분석물농도지간유량호적선성관계.응용본방법측정2충중약화풍습성심장병환자혈장중적신상선색화다파알,회수솔위95.8%～105.6%.
A microemulsion electrokinetic chromatography method with laser induced fluorescence de-tection was established to analyze epinephrine and dopamine after their having been derivatizated with 4-chloro-7-nitrobenzo-2-oxa-1, 3-diazol. A stock microemulsion solution composed of w(SDS) : w(1-butanol) : w(n-heptane) : w (water)=3.31 : 6.61 : 0.80 : 89.28 was prepared. The optimum separation conditions were as follows: the running buffer was composed of 20 mmol/L borate+20% microemulison (V/V)+4% ace-tonitrile (adjust pH to 9.3) and a voltage of 20 kV was applied. Good linearity relationships between peak areas and concentration of the analytes were obtained. The method was applied to the analysis of the two compounds in two traditional Chinese medicines and rheumatic heart disease patients' plasma with recoveries in the range of 95.8%～105.6%.