中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2009年
7期
1113-1118
,共6页
唐成武%鲍鹰%朱鸣%温晓红%费卯云%张晓岚%陶玉龙%王耀%秦连进%冯文明
唐成武%鮑鷹%硃鳴%溫曉紅%費卯雲%張曉嵐%陶玉龍%王耀%秦連進%馮文明
당성무%포응%주명%온효홍%비묘운%장효람%도옥룡%왕요%진련진%풍문명
脓毒症%脑啡肽,亮氨酸-2-丙氨酸%疾病模型,动物%腺苷三磷酸%线粒体,肝
膿毒癥%腦啡肽,亮氨痠-2-丙氨痠%疾病模型,動物%腺苷三燐痠%線粒體,肝
농독증%뇌배태,량안산-2-병안산%질병모형,동물%선감삼린산%선립체,간
Sepsis%Enkephalin,leucine-2-alanine%Disease models,animal%Adenosine triphosphate%Mitochondria,liver
目的 探讨δ阿片受体激动剂DADLE(D-Ala2-D-Leu5-enkephali)对脓毒症大鼠肝脏的保护作用.方法 SD大鼠60只,分为假手术组(SO组)、脓毒症组(SEP组)和DADLE(5 mg/kg)给药处理组(DADLE组),每组20只.采用改良盲肠结扎穿孔方法(CLP)建立大鼠脓毒症模型,SO组除不结扎刺穿盲肠外,其余操作同SEP组,DADLE组模型建立后立即按5 ml/kg剂量静脉注射浓度为0.5 mg/ml的DADLE.制模8 h后处死大鼠,取血检测ALT和AST水平.采用Clark氧电极技术测定肝细胞线粒体呼吸功能,高效液相色谱法测定肝组织ATP、ADP、AMP含量.观察各组大鼠肝组织病理形态改变.结果 与SO组比较其余两组ALT和AST明显升高,而DADLE组与SEP组比有明显降低(P<0.05).给予DADLE治疗的大鼠呼吸控制率(RCR)、磷/氧化(ADP/O)与SEP组比较显著升高(P<0.05).组织病理学提示DADLE组大鼠肝脏组织坏死灶及炎细胞浸润明显轻于SEP组.结论 δ阿片受体激动剂DADLE对脓毒症大鼠肝脏具有一定的保护作用,其机制可能与保护线粒体呼吸功能,减轻线粒体水肿,改善肝细胞能量代谢有关.
目的 探討δ阿片受體激動劑DADLE(D-Ala2-D-Leu5-enkephali)對膿毒癥大鼠肝髒的保護作用.方法 SD大鼠60隻,分為假手術組(SO組)、膿毒癥組(SEP組)和DADLE(5 mg/kg)給藥處理組(DADLE組),每組20隻.採用改良盲腸結扎穿孔方法(CLP)建立大鼠膿毒癥模型,SO組除不結扎刺穿盲腸外,其餘操作同SEP組,DADLE組模型建立後立即按5 ml/kg劑量靜脈註射濃度為0.5 mg/ml的DADLE.製模8 h後處死大鼠,取血檢測ALT和AST水平.採用Clark氧電極技術測定肝細胞線粒體呼吸功能,高效液相色譜法測定肝組織ATP、ADP、AMP含量.觀察各組大鼠肝組織病理形態改變.結果 與SO組比較其餘兩組ALT和AST明顯升高,而DADLE組與SEP組比有明顯降低(P<0.05).給予DADLE治療的大鼠呼吸控製率(RCR)、燐/氧化(ADP/O)與SEP組比較顯著升高(P<0.05).組織病理學提示DADLE組大鼠肝髒組織壞死竈及炎細胞浸潤明顯輕于SEP組.結論 δ阿片受體激動劑DADLE對膿毒癥大鼠肝髒具有一定的保護作用,其機製可能與保護線粒體呼吸功能,減輕線粒體水腫,改善肝細胞能量代謝有關.
목적 탐토δ아편수체격동제DADLE(D-Ala2-D-Leu5-enkephali)대농독증대서간장적보호작용.방법 SD대서60지,분위가수술조(SO조)、농독증조(SEP조)화DADLE(5 mg/kg)급약처리조(DADLE조),매조20지.채용개량맹장결찰천공방법(CLP)건립대서농독증모형,SO조제불결찰자천맹장외,기여조작동SEP조,DADLE조모형건립후립즉안5 ml/kg제량정맥주사농도위0.5 mg/ml적DADLE.제모8 h후처사대서,취혈검측ALT화AST수평.채용Clark양전겁기술측정간세포선립체호흡공능,고효액상색보법측정간조직ATP、ADP、AMP함량.관찰각조대서간조직병리형태개변.결과 여SO조비교기여량조ALT화AST명현승고,이DADLE조여SEP조비유명현강저(P<0.05).급여DADLE치료적대서호흡공제솔(RCR)、린/양화(ADP/O)여SEP조비교현저승고(P<0.05).조직병이학제시DADLE조대서간장조직배사조급염세포침윤명현경우SEP조.결론 δ아편수체격동제DADLE대농독증대서간장구유일정적보호작용,기궤제가능여보호선립체호흡공능,감경선립체수종,개선간세포능량대사유관.
Objective To study the hepatoprotective effect of DADLE(D-Ala2-D-Leu5-enkephali) in rat with sepsis.Methods Sixty SD rats were randomly divided in to sham operated group (SO),septic group(SEP),and DADLE group (DADLE).Sepsis model was produced by cecal ligation and puncture (CLP).In SO group,the abdomen was opened without any other treatment.In DADLE group,DADLE(0.5 mg/ml) was administer at a dose of 5 ml/kg by intravenous injection after CLP.All rats were sacrificed 8 h after CLP.ALT and AST in blood were determined.The function of mitochodria was evaluated by the clark oxygen eletrode.The levels of ATP,ADP,AMP were determined by high performance liquid chromatography (HPLC).The changes of mitochondrial ultrastructure were observed by using electron microscope.Results ALT and AST of DADLE groups were significantly lower than SEP group(P<0.05),while RCR and ADP/O were much higher than SEP group(P<0.05).ATP of DADLE groups were much higher than sep group(P<0.05).The damage of hepatocellular mitochondrial structure and function of SEP group were much severe than DADLE groups.Conclusions DADLE has hepatoprotective effect in rats with sepsis.
